Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03365 and AZOBR_RS03370 are separated by 6 nucleotides AZOBR_RS03370 and AZOBR_RS03375 are separated by 13 nucleotides
AZOBR_RS03365: AZOBR_RS03365 - glycosyl transferase, at 712,918 to 714,189
_RS03365
AZOBR_RS03370: AZOBR_RS03370 - GDP-L-fucose synthase, at 714,196 to 715,176
_RS03370
AZOBR_RS03375: AZOBR_RS03375 - NAD-dependent dehydratase, at 715,190 to 716,191
_RS03375
Position (kb) 714
715
716 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 713.463 kb on - strand, within AZOBR_RS03365 at 713.972 kb on + strand, within AZOBR_RS03365 at 713.972 kb on + strand, within AZOBR_RS03365 at 713.972 kb on + strand, within AZOBR_RS03365 at 713.973 kb on - strand, within AZOBR_RS03365 at 713.973 kb on - strand, within AZOBR_RS03365 at 713.973 kb on - strand, within AZOBR_RS03365 at 714.092 kb on + strand at 714.092 kb on + strand at 714.092 kb on + strand at 714.092 kb on + strand at 714.093 kb on - strand at 714.275 kb on + strand at 714.276 kb on - strand at 714.405 kb on - strand, within AZOBR_RS03370 at 714.491 kb on + strand, within AZOBR_RS03370 at 714.491 kb on + strand, within AZOBR_RS03370 at 714.492 kb on - strand, within AZOBR_RS03370 at 714.492 kb on - strand, within AZOBR_RS03370 at 714.537 kb on - strand, within AZOBR_RS03370 at 714.674 kb on + strand, within AZOBR_RS03370 at 714.674 kb on + strand, within AZOBR_RS03370 at 714.767 kb on + strand, within AZOBR_RS03370 at 714.768 kb on - strand, within AZOBR_RS03370 at 715.001 kb on + strand, within AZOBR_RS03370 at 715.001 kb on + strand, within AZOBR_RS03370 at 715.002 kb on - strand, within AZOBR_RS03370 at 715.002 kb on - strand, within AZOBR_RS03370 at 715.192 kb on - strand at 715.452 kb on + strand, within AZOBR_RS03375 at 715.452 kb on + strand, within AZOBR_RS03375 at 715.452 kb on + strand, within AZOBR_RS03375 at 715.452 kb on + strand, within AZOBR_RS03375 at 715.452 kb on + strand, within AZOBR_RS03375 at 715.452 kb on + strand, within AZOBR_RS03375 at 715.452 kb on + strand, within AZOBR_RS03375 at 715.453 kb on - strand, within AZOBR_RS03375 at 715.453 kb on - strand, within AZOBR_RS03375 at 715.453 kb on - strand, within AZOBR_RS03375 at 715.453 kb on - strand, within AZOBR_RS03375 at 715.651 kb on - strand, within AZOBR_RS03375 at 715.743 kb on + strand, within AZOBR_RS03375 at 715.750 kb on - strand, within AZOBR_RS03375 at 715.822 kb on - strand, within AZOBR_RS03375 at 715.936 kb on - strand, within AZOBR_RS03375 at 715.936 kb on - strand, within AZOBR_RS03375 at 715.936 kb on - strand, within AZOBR_RS03375
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 713,463 - AZOBR_RS03365 0.43 -3.3 713,972 + AZOBR_RS03365 0.83 -3.4 713,972 + AZOBR_RS03365 0.83 -3.5 713,972 + AZOBR_RS03365 0.83 -0.0 713,973 - AZOBR_RS03365 0.83 -0.7 713,973 - AZOBR_RS03365 0.83 +0.3 713,973 - AZOBR_RS03365 0.83 -1.1 714,092 + +0.7 714,092 + +1.7 714,092 + -0.8 714,092 + +0.3 714,093 - +1.2 714,275 + +1.4 714,276 - +0.4 714,405 - AZOBR_RS03370 0.21 -2.8 714,491 + AZOBR_RS03370 0.30 -0.8 714,491 + AZOBR_RS03370 0.30 -0.5 714,492 - AZOBR_RS03370 0.30 +1.7 714,492 - AZOBR_RS03370 0.30 -0.3 714,537 - AZOBR_RS03370 0.35 +0.7 714,674 + AZOBR_RS03370 0.49 -3.2 714,674 + AZOBR_RS03370 0.49 -2.5 714,767 + AZOBR_RS03370 0.58 +1.0 714,768 - AZOBR_RS03370 0.58 +2.0 715,001 + AZOBR_RS03370 0.82 -0.9 715,001 + AZOBR_RS03370 0.82 +1.3 715,002 - AZOBR_RS03370 0.82 +0.6 715,002 - AZOBR_RS03370 0.82 -0.7 715,192 - +2.2 715,452 + AZOBR_RS03375 0.26 +0.8 715,452 + AZOBR_RS03375 0.26 +0.6 715,452 + AZOBR_RS03375 0.26 +0.4 715,452 + AZOBR_RS03375 0.26 +0.8 715,452 + AZOBR_RS03375 0.26 -3.5 715,452 + AZOBR_RS03375 0.26 -0.5 715,452 + AZOBR_RS03375 0.26 -1.2 715,453 - AZOBR_RS03375 0.26 +1.3 715,453 - AZOBR_RS03375 0.26 +0.6 715,453 - AZOBR_RS03375 0.26 -0.7 715,453 - AZOBR_RS03375 0.26 -1.5 715,651 - AZOBR_RS03375 0.46 -0.4 715,743 + AZOBR_RS03375 0.55 +2.0 715,750 - AZOBR_RS03375 0.56 -0.7 715,822 - AZOBR_RS03375 0.63 -0.8 715,936 - AZOBR_RS03375 0.74 +0.3 715,936 - AZOBR_RS03375 0.74 -0.1 715,936 - AZOBR_RS03375 0.74 -3.7
Or see this region's nucleotide sequence