Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03105 and AZOBR_RS03110 are separated by 322 nucleotides AZOBR_RS03110 and AZOBR_RS03115 are separated by 88 nucleotides AZOBR_RS03115 and AZOBR_RS03120 are separated by 88 nucleotides
AZOBR_RS03105: AZOBR_RS03105 - membrane protein, at 663,390 to 664,160
_RS03105
AZOBR_RS03110: AZOBR_RS03110 - integration host factor subunit beta, at 664,483 to 664,767
_RS03110
AZOBR_RS03115: AZOBR_RS03115 - hypothetical protein, at 664,856 to 665,245
_RS03115
AZOBR_RS03120: AZOBR_RS03120 - ornithine cyclodeaminase, at 665,334 to 666,290
_RS03120
Position (kb) 664
665 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 663.802 kb on + strand, within AZOBR_RS03105 at 663.802 kb on + strand, within AZOBR_RS03105 at 663.802 kb on + strand, within AZOBR_RS03105 at 663.802 kb on + strand, within AZOBR_RS03105 at 663.803 kb on - strand, within AZOBR_RS03105 at 663.803 kb on - strand, within AZOBR_RS03105 at 663.803 kb on - strand, within AZOBR_RS03105 at 663.803 kb on - strand, within AZOBR_RS03105 at 663.803 kb on - strand, within AZOBR_RS03105 at 663.803 kb on - strand, within AZOBR_RS03105 at 663.979 kb on + strand, within AZOBR_RS03105 at 663.980 kb on - strand, within AZOBR_RS03105 at 663.980 kb on - strand, within AZOBR_RS03105 at 664.144 kb on + strand at 664.391 kb on + strand at 664.391 kb on + strand at 664.534 kb on + strand, within AZOBR_RS03110 at 664.534 kb on + strand at 664.535 kb on - strand, within AZOBR_RS03110 at 664.840 kb on + strand at 664.841 kb on - strand at 665.097 kb on - strand, within AZOBR_RS03115 at 665.097 kb on - strand, within AZOBR_RS03115 at 665.587 kb on - strand, within AZOBR_RS03120 at 665.688 kb on + strand, within AZOBR_RS03120 at 665.689 kb on - strand, within AZOBR_RS03120
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 663,802 + AZOBR_RS03105 0.53 +0.1 663,802 + AZOBR_RS03105 0.53 -1.2 663,802 + AZOBR_RS03105 0.53 +0.1 663,802 + AZOBR_RS03105 0.53 -0.2 663,803 - AZOBR_RS03105 0.54 -1.7 663,803 - AZOBR_RS03105 0.54 -1.4 663,803 - AZOBR_RS03105 0.54 -0.2 663,803 - AZOBR_RS03105 0.54 -4.5 663,803 - AZOBR_RS03105 0.54 -0.7 663,803 - AZOBR_RS03105 0.54 +1.5 663,979 + AZOBR_RS03105 0.76 +1.0 663,980 - AZOBR_RS03105 0.77 -2.4 663,980 - AZOBR_RS03105 0.77 +1.4 664,144 + -0.4 664,391 + -0.9 664,391 + -0.1 664,534 + AZOBR_RS03110 0.18 +0.2 664,534 + +2.4 664,535 - AZOBR_RS03110 0.18 -1.3 664,840 + -1.6 664,841 - +2.8 665,097 - AZOBR_RS03115 0.62 -1.3 665,097 - AZOBR_RS03115 0.62 -1.2 665,587 - AZOBR_RS03120 0.26 +1.8 665,688 + AZOBR_RS03120 0.37 -1.8 665,689 - AZOBR_RS03120 0.37 -0.7
Or see this region's nucleotide sequence