Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01970 and AZOBR_RS01975 overlap by 4 nucleotides AZOBR_RS01975 and AZOBR_RS01980 are separated by 461 nucleotides
AZOBR_RS01970: AZOBR_RS01970 - membrane protein, at 403,898 to 405,130
_RS01970
AZOBR_RS01975: AZOBR_RS01975 - glycosyl transferase, at 405,127 to 406,401
_RS01975
AZOBR_RS01980: AZOBR_RS01980 - hypothetical protein, at 406,863 to 407,423
_RS01980
Position (kb) 405
406
407 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 404.204 kb on + strand, within AZOBR_RS01970 at 404.204 kb on + strand, within AZOBR_RS01970 at 404.483 kb on + strand, within AZOBR_RS01970 at 404.484 kb on - strand, within AZOBR_RS01970 at 404.492 kb on + strand, within AZOBR_RS01970 at 404.552 kb on + strand, within AZOBR_RS01970 at 404.553 kb on - strand, within AZOBR_RS01970 at 404.553 kb on - strand, within AZOBR_RS01970 at 404.553 kb on - strand, within AZOBR_RS01970 at 404.618 kb on + strand, within AZOBR_RS01970 at 404.618 kb on + strand, within AZOBR_RS01970 at 404.801 kb on + strand, within AZOBR_RS01970 at 404.801 kb on + strand, within AZOBR_RS01970 at 404.801 kb on + strand, within AZOBR_RS01970 at 404.802 kb on - strand, within AZOBR_RS01970 at 405.062 kb on + strand at 405.299 kb on - strand, within AZOBR_RS01975 at 405.299 kb on - strand, within AZOBR_RS01975 at 405.299 kb on - strand, within AZOBR_RS01975 at 405.311 kb on - strand, within AZOBR_RS01975 at 405.535 kb on + strand, within AZOBR_RS01975 at 405.536 kb on - strand, within AZOBR_RS01975 at 406.857 kb on + strand at 406.857 kb on + strand at 406.858 kb on - strand at 406.858 kb on - strand at 406.858 kb on - strand at 406.858 kb on - strand at 407.362 kb on - strand, within AZOBR_RS01980 at 407.362 kb on - strand, within AZOBR_RS01980
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 404,204 + AZOBR_RS01970 0.25 +1.6 404,204 + AZOBR_RS01970 0.25 -0.3 404,483 + AZOBR_RS01970 0.47 -0.7 404,484 - AZOBR_RS01970 0.48 +2.9 404,492 + AZOBR_RS01970 0.48 -0.0 404,552 + AZOBR_RS01970 0.53 +0.7 404,553 - AZOBR_RS01970 0.53 +1.6 404,553 - AZOBR_RS01970 0.53 +0.7 404,553 - AZOBR_RS01970 0.53 -4.2 404,618 + AZOBR_RS01970 0.58 +2.2 404,618 + AZOBR_RS01970 0.58 +0.2 404,801 + AZOBR_RS01970 0.73 +1.7 404,801 + AZOBR_RS01970 0.73 +0.7 404,801 + AZOBR_RS01970 0.73 +0.7 404,802 - AZOBR_RS01970 0.73 -2.5 405,062 + +1.7 405,299 - AZOBR_RS01975 0.13 +0.5 405,299 - AZOBR_RS01975 0.13 +0.8 405,299 - AZOBR_RS01975 0.13 +1.1 405,311 - AZOBR_RS01975 0.14 +1.0 405,535 + AZOBR_RS01975 0.32 +0.2 405,536 - AZOBR_RS01975 0.32 +1.8 406,857 + +1.7 406,857 + -2.4 406,858 - -0.8 406,858 - -0.1 406,858 - -0.8 406,858 - -2.9 407,362 - AZOBR_RS01980 0.89 +1.7 407,362 - AZOBR_RS01980 0.89 +1.6
Or see this region's nucleotide sequence