Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01575 and AZOBR_RS01580 are separated by 19 nucleotides AZOBR_RS01580 and AZOBR_RS01585 are separated by 71 nucleotides AZOBR_RS01585 and AZOBR_RS01590 are separated by 158 nucleotides AZOBR_RS01590 and AZOBR_RS01595 overlap by 8 nucleotides
AZOBR_RS01575: AZOBR_RS01575 - 6-O-methylguanine DNA methyltransferase, at 323,345 to 324,169
_RS01575
AZOBR_RS01580: AZOBR_RS01580 - dihydrodipicolinate reductase, at 324,189 to 324,986
_RS01580
AZOBR_RS01585: AZOBR_RS01585 - zinc transporter, at 325,058 to 326,044
_RS01585
AZOBR_RS01590: AZOBR_RS01590 - zinc ABC transporter ATP-binding protein, at 326,203 to 326,964
_RS01590
AZOBR_RS01595: AZOBR_RS01595 - zinc transporter, at 326,957 to 327,745
_RS01595
Position (kb) 325
326
327 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.167 kb on + strand at 325.339 kb on - strand, within AZOBR_RS01585 at 325.353 kb on + strand, within AZOBR_RS01585 at 325.359 kb on + strand, within AZOBR_RS01585 at 325.360 kb on - strand, within AZOBR_RS01585 at 325.470 kb on + strand, within AZOBR_RS01585 at 325.618 kb on + strand, within AZOBR_RS01585 at 325.618 kb on + strand, within AZOBR_RS01585 at 325.619 kb on - strand, within AZOBR_RS01585 at 325.619 kb on - strand, within AZOBR_RS01585 at 325.619 kb on - strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.846 kb on - strand, within AZOBR_RS01585 at 325.846 kb on - strand, within AZOBR_RS01585 at 326.093 kb on + strand at 326.093 kb on + strand at 326.093 kb on + strand at 326.093 kb on + strand at 326.093 kb on + strand at 326.093 kb on + strand at 326.094 kb on - strand at 326.094 kb on - strand at 326.094 kb on - strand at 326.094 kb on - strand at 326.094 kb on - strand at 326.417 kb on - strand, within AZOBR_RS01590 at 326.417 kb on - strand, within AZOBR_RS01590 at 326.884 kb on + strand, within AZOBR_RS01590 at 326.884 kb on + strand, within AZOBR_RS01590 at 326.885 kb on - strand, within AZOBR_RS01590 at 326.885 kb on - strand, within AZOBR_RS01590 at 326.885 kb on - strand, within AZOBR_RS01590 at 326.885 kb on - strand, within AZOBR_RS01590
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 324,086 + AZOBR_RS01575 0.90 -0.2 324,086 + AZOBR_RS01575 0.90 +2.0 324,167 + -2.7 325,339 - AZOBR_RS01585 0.28 +0.3 325,353 + AZOBR_RS01585 0.30 -0.7 325,359 + AZOBR_RS01585 0.30 +0.3 325,360 - AZOBR_RS01585 0.31 +2.6 325,470 + AZOBR_RS01585 0.42 +0.6 325,618 + AZOBR_RS01585 0.57 -4.4 325,618 + AZOBR_RS01585 0.57 -2.6 325,619 - AZOBR_RS01585 0.57 +1.5 325,619 - AZOBR_RS01585 0.57 -2.0 325,619 - AZOBR_RS01585 0.57 -0.8 325,845 + AZOBR_RS01585 0.80 -0.5 325,845 + AZOBR_RS01585 0.80 -0.5 325,845 + AZOBR_RS01585 0.80 -1.7 325,845 + AZOBR_RS01585 0.80 +0.5 325,845 + AZOBR_RS01585 0.80 -0.4 325,846 - AZOBR_RS01585 0.80 -4.6 325,846 - AZOBR_RS01585 0.80 -1.1 326,093 + -1.2 326,093 + +2.4 326,093 + +0.0 326,093 + +0.6 326,093 + +0.3 326,093 + -1.1 326,094 - -1.2 326,094 - -1.5 326,094 - -4.2 326,094 - -0.4 326,094 - +0.8 326,417 - AZOBR_RS01590 0.28 -0.4 326,417 - AZOBR_RS01590 0.28 -0.4 326,884 + AZOBR_RS01590 0.89 +0.6 326,884 + AZOBR_RS01590 0.89 +0.2 326,885 - AZOBR_RS01590 0.90 -5.1 326,885 - AZOBR_RS01590 0.90 -2.0 326,885 - AZOBR_RS01590 0.90 -0.7 326,885 - AZOBR_RS01590 0.90 -0.1
Or see this region's nucleotide sequence