Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01005 and AZOBR_RS01010 are separated by 162 nucleotides AZOBR_RS01010 and AZOBR_RS01015 are separated by 78 nucleotides AZOBR_RS01015 and AZOBR_RS01020 are separated by 137 nucleotides AZOBR_RS01020 and AZOBR_RS01025 overlap by 6 nucleotides
AZOBR_RS01005: AZOBR_RS01005 - Fur family transcriptional regulator, at 204,691 to 205,125
_RS01005
AZOBR_RS01010: AZOBR_RS01010 - hypothetical protein, at 205,288 to 205,530
_RS01010
AZOBR_RS01015: AZOBR_RS01015 - haloacid dehalogenase, at 205,609 to 206,274
_RS01015
AZOBR_RS01020: AZOBR_RS01020 - hypothetical protein, at 206,412 to 206,810
_RS01020
AZOBR_RS01025: AZOBR_RS01025 - hypothetical protein, at 206,805 to 207,533
_RS01025
Position (kb) 205
206
207 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 204.642 kb on + strand at 205.078 kb on + strand at 205.079 kb on - strand at 205.243 kb on + strand at 205.243 kb on + strand at 205.243 kb on + strand at 205.243 kb on + strand at 205.244 kb on - strand at 205.528 kb on + strand at 206.035 kb on + strand, within AZOBR_RS01015 at 206.035 kb on + strand, within AZOBR_RS01015 at 206.035 kb on + strand, within AZOBR_RS01015 at 206.035 kb on + strand, within AZOBR_RS01015 at 206.035 kb on + strand, within AZOBR_RS01015 at 206.035 kb on + strand, within AZOBR_RS01015 at 206.036 kb on - strand, within AZOBR_RS01015 at 206.036 kb on - strand, within AZOBR_RS01015 at 206.036 kb on - strand, within AZOBR_RS01015 at 206.036 kb on - strand, within AZOBR_RS01015 at 206.036 kb on - strand, within AZOBR_RS01015 at 206.369 kb on - strand at 206.806 kb on + strand at 206.809 kb on - strand at 206.809 kb on - strand at 206.809 kb on - strand at 207.043 kb on + strand, within AZOBR_RS01025 at 207.043 kb on + strand, within AZOBR_RS01025
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 204,642 + -5.2 205,078 + -0.3 205,079 - +0.7 205,243 + -0.1 205,243 + +0.7 205,243 + -3.3 205,243 + -1.4 205,244 - +0.7 205,528 + -2.4 206,035 + AZOBR_RS01015 0.64 -2.4 206,035 + AZOBR_RS01015 0.64 -0.6 206,035 + AZOBR_RS01015 0.64 -2.3 206,035 + AZOBR_RS01015 0.64 +1.0 206,035 + AZOBR_RS01015 0.64 -0.2 206,035 + AZOBR_RS01015 0.64 -1.6 206,036 - AZOBR_RS01015 0.64 -2.2 206,036 - AZOBR_RS01015 0.64 -0.5 206,036 - AZOBR_RS01015 0.64 -3.8 206,036 - AZOBR_RS01015 0.64 -0.4 206,036 - AZOBR_RS01015 0.64 -1.9 206,369 - -0.5 206,806 + +0.4 206,809 - +0.3 206,809 - +0.4 206,809 - +0.2 207,043 + AZOBR_RS01025 0.33 +1.5 207,043 + AZOBR_RS01025 0.33 +1.0
Or see this region's nucleotide sequence