Experiment: MoYLS4 with Doxycycline hyclate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2980 and DvMF_2981 are separated by 110 nucleotides DvMF_2981 and DvMF_2982 are separated by 181 nucleotides DvMF_2982 and DvMF_2983 are separated by 97 nucleotides DvMF_2983 and DvMF_2984 are separated by 176 nucleotides
DvMF_2980: DvMF_2980 - Rhodanese domain protein (RefSeq), at 3,754,410 to 3,754,916
_2980
DvMF_2981: DvMF_2981 - VanZ family protein (RefSeq), at 3,755,027 to 3,755,596
_2981
DvMF_2982: DvMF_2982 - SlyX family protein (RefSeq), at 3,755,778 to 3,755,999
_2982
DvMF_2983: DvMF_2983 - protein of unknown function DUF55 (RefSeq), at 3,756,097 to 3,756,564
_2983
DvMF_2984: DvMF_2984 - hypothetical protein (RefSeq), at 3,756,741 to 3,759,431
_2984
Position (kb)
3755
3756 Strain fitness (log2 ratio)
-2
-1
0
1 at 3754.867 kb on + strand at 3755.058 kb on + strand at 3755.066 kb on - strand at 3755.328 kb on - strand, within DvMF_2981 at 3755.354 kb on + strand, within DvMF_2981 at 3755.551 kb on - strand at 3755.598 kb on - strand at 3755.979 kb on + strand at 3756.027 kb on - strand at 3756.238 kb on - strand, within DvMF_2983 at 3756.355 kb on - strand, within DvMF_2983 at 3756.422 kb on - strand, within DvMF_2983 at 3756.422 kb on - strand, within DvMF_2983 at 3756.539 kb on + strand at 3756.616 kb on + strand at 3756.859 kb on - strand at 3756.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.003 mg/ml remove 3,754,867 + -0.7 3,755,058 + +0.4 3,755,066 - -2.0 3,755,328 - DvMF_2981 0.53 -1.5 3,755,354 + DvMF_2981 0.57 -1.0 3,755,551 - -0.4 3,755,598 - +0.2 3,755,979 + -0.9 3,756,027 - -2.2 3,756,238 - DvMF_2983 0.30 -0.6 3,756,355 - DvMF_2983 0.55 -0.5 3,756,422 - DvMF_2983 0.69 -0.9 3,756,422 - DvMF_2983 0.69 -1.1 3,756,539 + -1.5 3,756,616 + -1.8 3,756,859 - -0.6 3,756,980 - -1.3
Or see this region's nucleotide sequence