Experiment: MoYLS4 with Doxycycline hyclate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0547 and DvMF_0548 are separated by 96 nucleotides DvMF_0548 and DvMF_0549 are separated by 136 nucleotides DvMF_0549 and DvMF_0550 are separated by 159 nucleotides
DvMF_0547: DvMF_0547 - putative carboxylesterase (RefSeq), at 644,939 to 645,748
_0547
DvMF_0548: DvMF_0548 - Guanine deaminase (RefSeq), at 645,845 to 647,167
_0548
DvMF_0549: DvMF_0549 - hypothetical protein (RefSeq), at 647,304 to 647,579
_0549
DvMF_0550: DvMF_0550 - diaminopimelate aminotransferase (RefSeq), at 647,739 to 648,968
_0550
Position (kb) 645
646
647
648 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 645.050 kb on - strand, within DvMF_0547 at 645.099 kb on + strand, within DvMF_0547 at 645.172 kb on - strand, within DvMF_0547 at 645.196 kb on + strand, within DvMF_0547 at 645.287 kb on + strand, within DvMF_0547 at 645.288 kb on - strand, within DvMF_0547 at 645.430 kb on + strand, within DvMF_0547 at 645.512 kb on - strand, within DvMF_0547 at 645.558 kb on + strand, within DvMF_0547 at 645.749 kb on + strand at 645.853 kb on + strand at 645.872 kb on - strand at 645.981 kb on - strand, within DvMF_0548 at 646.064 kb on + strand, within DvMF_0548 at 646.064 kb on + strand, within DvMF_0548 at 646.064 kb on + strand, within DvMF_0548 at 646.072 kb on - strand, within DvMF_0548 at 646.178 kb on + strand, within DvMF_0548 at 646.402 kb on + strand, within DvMF_0548 at 646.429 kb on - strand, within DvMF_0548 at 646.429 kb on - strand, within DvMF_0548 at 646.431 kb on - strand, within DvMF_0548 at 646.661 kb on + strand, within DvMF_0548 at 646.836 kb on + strand, within DvMF_0548 at 646.914 kb on - strand, within DvMF_0548 at 646.949 kb on + strand, within DvMF_0548 at 647.108 kb on + strand at 647.111 kb on - strand at 648.067 kb on - strand, within DvMF_0550 at 648.111 kb on - strand, within DvMF_0550
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.003 mg/ml remove 645,050 - DvMF_0547 0.14 -3.5 645,099 + DvMF_0547 0.20 -0.9 645,172 - DvMF_0547 0.29 -0.1 645,196 + DvMF_0547 0.32 -2.3 645,287 + DvMF_0547 0.43 -1.2 645,288 - DvMF_0547 0.43 -0.4 645,430 + DvMF_0547 0.61 -0.2 645,512 - DvMF_0547 0.71 -4.4 645,558 + DvMF_0547 0.76 -2.4 645,749 + -1.3 645,853 + +0.1 645,872 - +0.9 645,981 - DvMF_0548 0.10 -1.4 646,064 + DvMF_0548 0.17 -2.6 646,064 + DvMF_0548 0.17 -1.1 646,064 + DvMF_0548 0.17 -1.7 646,072 - DvMF_0548 0.17 -0.3 646,178 + DvMF_0548 0.25 +0.0 646,402 + DvMF_0548 0.42 -1.1 646,429 - DvMF_0548 0.44 +0.2 646,429 - DvMF_0548 0.44 -1.2 646,431 - DvMF_0548 0.44 -1.8 646,661 + DvMF_0548 0.62 -0.6 646,836 + DvMF_0548 0.75 -0.2 646,914 - DvMF_0548 0.81 -0.5 646,949 + DvMF_0548 0.83 -2.4 647,108 + +0.1 647,111 - -0.6 648,067 - DvMF_0550 0.27 -2.6 648,111 - DvMF_0550 0.30 -0.5
Or see this region's nucleotide sequence