Experiment: MoYLS4 with Doxycycline hyclate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0545 and DvMF_0546 are separated by 216 nucleotides DvMF_0546 and DvMF_0547 are separated by 401 nucleotides
DvMF_0545: DvMF_0545 - glutamine amidotransferase class-II (RefSeq), at 641,688 to 642,797
_0545
DvMF_0546: DvMF_0546 - Glutamate synthase (NADPH) (RefSeq), at 643,014 to 644,537
_0546
DvMF_0547: DvMF_0547 - putative carboxylesterase (RefSeq), at 644,939 to 645,748
_0547
Position (kb) 643
644
645 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 642.033 kb on - strand, within DvMF_0545 at 642.180 kb on - strand, within DvMF_0545 at 642.588 kb on - strand, within DvMF_0545 at 642.598 kb on + strand, within DvMF_0545 at 642.703 kb on - strand at 643.036 kb on - strand at 643.059 kb on + strand at 643.145 kb on - strand at 643.190 kb on - strand, within DvMF_0546 at 643.352 kb on + strand, within DvMF_0546 at 643.357 kb on + strand, within DvMF_0546 at 643.370 kb on - strand, within DvMF_0546 at 643.416 kb on + strand, within DvMF_0546 at 643.433 kb on - strand, within DvMF_0546 at 643.440 kb on - strand, within DvMF_0546 at 643.633 kb on + strand, within DvMF_0546 at 643.641 kb on - strand, within DvMF_0546 at 643.839 kb on - strand, within DvMF_0546 at 644.399 kb on - strand at 644.424 kb on + strand at 644.541 kb on + strand at 644.610 kb on - strand at 644.661 kb on + strand at 645.050 kb on - strand, within DvMF_0547 at 645.099 kb on + strand, within DvMF_0547 at 645.172 kb on - strand, within DvMF_0547 at 645.196 kb on + strand, within DvMF_0547 at 645.287 kb on + strand, within DvMF_0547 at 645.288 kb on - strand, within DvMF_0547 at 645.430 kb on + strand, within DvMF_0547 at 645.512 kb on - strand, within DvMF_0547
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.003 mg/ml remove 642,033 - DvMF_0545 0.31 +1.1 642,180 - DvMF_0545 0.44 -0.7 642,588 - DvMF_0545 0.81 +0.2 642,598 + DvMF_0545 0.82 -0.1 642,703 - +0.0 643,036 - -0.8 643,059 + -1.0 643,145 - -1.1 643,190 - DvMF_0546 0.12 -0.3 643,352 + DvMF_0546 0.22 -2.5 643,357 + DvMF_0546 0.23 -0.3 643,370 - DvMF_0546 0.23 -0.3 643,416 + DvMF_0546 0.26 +1.2 643,433 - DvMF_0546 0.27 -0.3 643,440 - DvMF_0546 0.28 -3.4 643,633 + DvMF_0546 0.41 -0.3 643,641 - DvMF_0546 0.41 -2.2 643,839 - DvMF_0546 0.54 -0.8 644,399 - -1.0 644,424 + +0.8 644,541 + -1.4 644,610 - -0.3 644,661 + -0.7 645,050 - DvMF_0547 0.14 -3.5 645,099 + DvMF_0547 0.20 -0.9 645,172 - DvMF_0547 0.29 -0.1 645,196 + DvMF_0547 0.32 -2.3 645,287 + DvMF_0547 0.43 -1.2 645,288 - DvMF_0547 0.43 -0.4 645,430 + DvMF_0547 0.61 -0.2 645,512 - DvMF_0547 0.71 -4.4
Or see this region's nucleotide sequence