Experiment: MoYLS4 with Doxycycline hyclate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0544 and DvMF_0545 overlap by 4 nucleotides DvMF_0545 and DvMF_0546 are separated by 216 nucleotides
DvMF_0544: DvMF_0544 - glutamate synthase alpha subunit domain protein (RefSeq), at 640,846 to 641,691
_0544
DvMF_0545: DvMF_0545 - glutamine amidotransferase class-II (RefSeq), at 641,688 to 642,797
_0545
DvMF_0546: DvMF_0546 - Glutamate synthase (NADPH) (RefSeq), at 643,014 to 644,537
_0546
Position (kb) 641
642
643 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 640.801 kb on + strand at 640.809 kb on - strand at 640.886 kb on + strand at 640.900 kb on - strand at 641.064 kb on + strand, within DvMF_0544 at 641.106 kb on - strand, within DvMF_0544 at 641.341 kb on - strand, within DvMF_0544 at 641.346 kb on - strand, within DvMF_0544 at 641.573 kb on - strand, within DvMF_0544 at 641.584 kb on - strand, within DvMF_0544 at 641.584 kb on - strand, within DvMF_0544 at 641.647 kb on - strand at 641.840 kb on + strand, within DvMF_0545 at 641.933 kb on - strand, within DvMF_0545 at 641.989 kb on + strand, within DvMF_0545 at 642.033 kb on - strand, within DvMF_0545 at 642.180 kb on - strand, within DvMF_0545 at 642.588 kb on - strand, within DvMF_0545 at 642.598 kb on + strand, within DvMF_0545 at 642.703 kb on - strand at 643.036 kb on - strand at 643.059 kb on + strand at 643.145 kb on - strand at 643.190 kb on - strand, within DvMF_0546 at 643.352 kb on + strand, within DvMF_0546 at 643.357 kb on + strand, within DvMF_0546 at 643.370 kb on - strand, within DvMF_0546 at 643.416 kb on + strand, within DvMF_0546 at 643.433 kb on - strand, within DvMF_0546 at 643.440 kb on - strand, within DvMF_0546 at 643.633 kb on + strand, within DvMF_0546 at 643.641 kb on - strand, within DvMF_0546
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.003 mg/ml remove 640,801 + -0.5 640,809 - -0.4 640,886 + -0.9 640,900 - -0.6 641,064 + DvMF_0544 0.26 -0.7 641,106 - DvMF_0544 0.31 -1.1 641,341 - DvMF_0544 0.59 -2.0 641,346 - DvMF_0544 0.59 -0.7 641,573 - DvMF_0544 0.86 +0.5 641,584 - DvMF_0544 0.87 -0.3 641,584 - DvMF_0544 0.87 -1.8 641,647 - -1.2 641,840 + DvMF_0545 0.14 +0.7 641,933 - DvMF_0545 0.22 -1.3 641,989 + DvMF_0545 0.27 -0.5 642,033 - DvMF_0545 0.31 +1.1 642,180 - DvMF_0545 0.44 -0.7 642,588 - DvMF_0545 0.81 +0.2 642,598 + DvMF_0545 0.82 -0.1 642,703 - +0.0 643,036 - -0.8 643,059 + -1.0 643,145 - -1.1 643,190 - DvMF_0546 0.12 -0.3 643,352 + DvMF_0546 0.22 -2.5 643,357 + DvMF_0546 0.23 -0.3 643,370 - DvMF_0546 0.23 -0.3 643,416 + DvMF_0546 0.26 +1.2 643,433 - DvMF_0546 0.27 -0.3 643,440 - DvMF_0546 0.28 -3.4 643,633 + DvMF_0546 0.41 -0.3 643,641 - DvMF_0546 0.41 -2.2
Or see this region's nucleotide sequence