Experiment: MoYLS4 with Doxycycline hyclate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0261 and DvMF_0262 are separated by 501 nucleotides DvMF_0262 and DvMF_0263 are separated by 33 nucleotides DvMF_0263 and DvMF_0264 are separated by 98 nucleotides DvMF_0264 and DvMF_0265 are separated by 28 nucleotides DvMF_0265 and ileS are separated by 117 nucleotides
DvMF_0261: DvMF_0261 - putative chemotaxis phosphatase, CheZ (RefSeq), at 299,020 to 299,766
_0261
DvMF_0262: DvMF_0262 - Homocysteine formation from aspartate semialdehyde (NIL/ferredoxin component) (from data) , at 300,268 to 300,711
_0262
DvMF_0263: DvMF_0263 - tol-pal system protein YbgF (RefSeq), at 300,745 to 301,746
_0263
DvMF_0264: DvMF_0264 - hypothetical protein (RefSeq), at 301,845 to 302,039
_0264
DvMF_0265: DvMF_0265 - lipoprotein signal peptidase (RefSeq), at 302,068 to 302,568
_0265
DvMF_0266: ileS - isoleucyl-tRNA synthetase (RefSeq), at 302,686 to 305,502
ileS
Position (kb)
300
301
302 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 299.770 kb on - strand at 299.938 kb on + strand at 299.938 kb on + strand at 299.983 kb on - strand at 300.141 kb on - strand at 300.256 kb on - strand at 300.311 kb on + strand at 300.328 kb on - strand, within DvMF_0262 at 300.521 kb on - strand, within DvMF_0262 at 300.606 kb on + strand, within DvMF_0262 at 300.689 kb on + strand at 301.781 kb on + strand at 301.806 kb on + strand at 302.025 kb on - strand at 302.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.003 mg/ml remove 299,770 - +0.6 299,938 + -0.3 299,938 + -1.3 299,983 - -0.5 300,141 - -0.4 300,256 - -0.7 300,311 + +0.4 300,328 - DvMF_0262 0.14 -0.4 300,521 - DvMF_0262 0.57 -1.6 300,606 + DvMF_0262 0.76 -2.6 300,689 + +0.4 301,781 + +0.8 301,806 + -0.7 302,025 - -3.2 302,064 - -0.2
Or see this region's nucleotide sequence