Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3069 and Shew_3070 are separated by 19 nucleotides Shew_3070 and Shew_3071 are separated by 357 nucleotides Shew_3071 and Shew_3072 are separated by 15 nucleotides
Shew_3069: Shew_3069 - 5-formyltetrahydrofolate cyclo-ligase (RefSeq), at 3,644,976 to 3,645,587
_3069
Shew_3070: Shew_3070 - extracellular solute-binding protein (RefSeq), at 3,645,607 to 3,646,467
_3070
Shew_3071: Shew_3071 - lipoprotein (RefSeq), at 3,646,825 to 3,647,334
_3071
Shew_3072: Shew_3072 - cold-shock protein, DNA-binding (RefSeq), at 3,647,350 to 3,647,943
_3072
Position (kb)
3645
3646
3647 Strain fitness (log2 ratio)
-1
0
1 at 3645.391 kb on - strand, within Shew_3069 at 3645.689 kb on - strand at 3645.848 kb on - strand, within Shew_3070 at 3645.977 kb on - strand, within Shew_3070 at 3646.067 kb on - strand, within Shew_3070 at 3646.461 kb on - strand at 3646.531 kb on + strand at 3646.531 kb on + strand at 3646.714 kb on - strand at 3646.770 kb on + strand at 3647.017 kb on + strand, within Shew_3071 at 3647.030 kb on - strand, within Shew_3071 at 3647.214 kb on - strand, within Shew_3071 at 3647.228 kb on - strand, within Shew_3071 at 3647.286 kb on + strand at 3647.286 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 3,645,391 - Shew_3069 0.68 +0.2 3,645,689 - -0.1 3,645,848 - Shew_3070 0.28 -0.6 3,645,977 - Shew_3070 0.43 -0.3 3,646,067 - Shew_3070 0.53 -1.2 3,646,461 - +0.7 3,646,531 + -0.0 3,646,531 + -0.6 3,646,714 - -0.5 3,646,770 + +0.9 3,647,017 + Shew_3071 0.38 -1.2 3,647,030 - Shew_3071 0.40 -1.0 3,647,214 - Shew_3071 0.76 +0.2 3,647,228 - Shew_3071 0.79 -0.0 3,647,286 + +0.2 3,647,286 + +0.4
Or see this region's nucleotide sequence