Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2697 and Shew_2698 are separated by 167 nucleotides Shew_2698 and Shew_2699 are separated by 221 nucleotides Shew_2699 and Shew_2700 are separated by 153 nucleotides
Shew_2697: Shew_2697 - hypothetical protein (RefSeq), at 3,192,364 to 3,193,740
_2697
Shew_2698: Shew_2698 - hypothetical protein (RefSeq), at 3,193,908 to 3,194,420
_2698
Shew_2699: Shew_2699 - cyclic nucleotide-binding protein (RefSeq), at 3,194,642 to 3,195,220
_2699
Shew_2700: Shew_2700 - antibiotic biosynthesis monooxygenase (RefSeq), at 3,195,374 to 3,195,763
_2700
Position (kb)
3193
3194
3195 Strain fitness (log2 ratio)
-2
-1
0
1 at 3193.022 kb on - strand, within Shew_2697 at 3193.108 kb on + strand, within Shew_2697 at 3193.223 kb on - strand, within Shew_2697 at 3193.501 kb on + strand, within Shew_2697 at 3193.927 kb on - strand at 3194.007 kb on - strand, within Shew_2698 at 3194.266 kb on + strand, within Shew_2698 at 3194.647 kb on - strand at 3194.663 kb on - strand at 3195.290 kb on - strand at 3195.338 kb on - strand at 3195.338 kb on - strand at 3195.338 kb on - strand at 3195.359 kb on + strand at 3195.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 3,193,022 - Shew_2697 0.48 -0.5 3,193,108 + Shew_2697 0.54 +0.6 3,193,223 - Shew_2697 0.62 -2.4 3,193,501 + Shew_2697 0.83 -0.2 3,193,927 - -0.5 3,194,007 - Shew_2698 0.19 -0.8 3,194,266 + Shew_2698 0.70 -0.8 3,194,647 - +0.6 3,194,663 - -0.4 3,195,290 - +0.2 3,195,338 - +0.7 3,195,338 - -1.6 3,195,338 - -0.1 3,195,359 + -0.6 3,195,407 - +0.2
Or see this region's nucleotide sequence