Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2592 and Shew_2593 are separated by 214 nucleotides Shew_2593 and Shew_2594 are separated by 108 nucleotides Shew_2594 and Shew_2595 are separated by 147 nucleotides
Shew_2592: Shew_2592 - peptidase M28 (RefSeq), at 3,057,319 to 3,058,905
_2592
Shew_2593: Shew_2593 - acyl-CoA synthetase (RefSeq), at 3,059,120 to 3,060,844
_2593
Shew_2594: Shew_2594 - hypothetical protein (RefSeq), at 3,060,953 to 3,061,225
_2594
Shew_2595: Shew_2595 - hypothetical protein (RefSeq), at 3,061,373 to 3,061,678
_2595
Position (kb)
3059
3060
3061 Strain fitness (log2 ratio)
-1
0
1 at 3058.692 kb on - strand, within Shew_2592 at 3058.856 kb on + strand at 3059.729 kb on - strand, within Shew_2593 at 3059.811 kb on - strand, within Shew_2593 at 3059.816 kb on + strand, within Shew_2593 at 3059.824 kb on - strand, within Shew_2593 at 3059.893 kb on + strand, within Shew_2593 at 3060.014 kb on - strand, within Shew_2593 at 3060.028 kb on + strand, within Shew_2593 at 3060.212 kb on - strand, within Shew_2593 at 3060.858 kb on + strand at 3061.016 kb on + strand, within Shew_2594 at 3061.043 kb on + strand, within Shew_2594 at 3061.195 kb on + strand, within Shew_2594 at 3061.323 kb on + strand at 3061.381 kb on + strand at 3061.497 kb on + strand, within Shew_2595 at 3061.507 kb on + strand, within Shew_2595 at 3061.642 kb on - strand, within Shew_2595 at 3061.653 kb on + strand at 3061.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 3,058,692 - Shew_2592 0.87 +0.3 3,058,856 + +0.0 3,059,729 - Shew_2593 0.35 +0.8 3,059,811 - Shew_2593 0.40 -0.9 3,059,816 + Shew_2593 0.40 +0.4 3,059,824 - Shew_2593 0.41 -1.0 3,059,893 + Shew_2593 0.45 -1.2 3,060,014 - Shew_2593 0.52 +0.0 3,060,028 + Shew_2593 0.53 -0.8 3,060,212 - Shew_2593 0.63 -0.5 3,060,858 + +0.8 3,061,016 + Shew_2594 0.23 +0.1 3,061,043 + Shew_2594 0.33 -0.5 3,061,195 + Shew_2594 0.89 -0.7 3,061,323 + +0.3 3,061,381 + +0.5 3,061,497 + Shew_2595 0.41 +0.2 3,061,507 + Shew_2595 0.44 -0.2 3,061,642 - Shew_2595 0.88 +0.3 3,061,653 + -0.0 3,061,661 - -0.2
Or see this region's nucleotide sequence