Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0429 and Shew_0430 are separated by 239 nucleotides Shew_0430 and Shew_0431 are separated by 254 nucleotides Shew_0431 and Shew_0432 are separated by 285 nucleotides Shew_0432 and Shew_0433 are separated by 21 nucleotides
Shew_0429: Shew_0429 - hypothetical protein (RefSeq), at 482,008 to 482,700
_0429
Shew_0430: Shew_0430 - histone family protein DNA-binding protein (RefSeq), at 482,940 to 483,212
_0430
Shew_0431: Shew_0431 - hypothetical protein (RefSeq), at 483,467 to 483,877
_0431
Shew_0432: Shew_0432 - hypothetical protein (RefSeq), at 484,163 to 484,672
_0432
Shew_0433: Shew_0433 - hypothetical protein (RefSeq), at 484,694 to 485,083
_0433
Position (kb) 483
484 Strain fitness (log2 ratio) -1
0
1 at 482.681 kb on - strand at 482.681 kb on - strand at 482.727 kb on + strand at 483.151 kb on + strand, within Shew_0430 at 483.465 kb on + strand at 483.473 kb on - strand at 483.479 kb on - strand at 483.549 kb on + strand, within Shew_0431 at 483.595 kb on + strand, within Shew_0431 at 483.623 kb on - strand, within Shew_0431 at 483.685 kb on - strand, within Shew_0431 at 483.709 kb on + strand, within Shew_0431 at 483.777 kb on - strand, within Shew_0431 at 483.778 kb on + strand, within Shew_0431 at 483.909 kb on + strand at 484.111 kb on - strand at 484.237 kb on + strand, within Shew_0432 at 484.417 kb on + strand, within Shew_0432 at 484.585 kb on + strand, within Shew_0432 at 484.774 kb on + strand, within Shew_0433 at 484.781 kb on - strand, within Shew_0433
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 482,681 - -0.8 482,681 - +1.1 482,727 + -0.9 483,151 + Shew_0430 0.77 +0.8 483,465 + -0.7 483,473 - -0.6 483,479 - -0.8 483,549 + Shew_0431 0.20 -0.9 483,595 + Shew_0431 0.31 +0.5 483,623 - Shew_0431 0.38 -1.5 483,685 - Shew_0431 0.53 -1.4 483,709 + Shew_0431 0.59 +0.2 483,777 - Shew_0431 0.75 +0.8 483,778 + Shew_0431 0.76 -0.2 483,909 + -0.5 484,111 - +0.5 484,237 + Shew_0432 0.15 +0.7 484,417 + Shew_0432 0.50 +0.1 484,585 + Shew_0432 0.83 +0.1 484,774 + Shew_0433 0.21 -1.2 484,781 - Shew_0433 0.22 +0.3
Or see this region's nucleotide sequence