Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0214 and Shew_0215 are separated by 16 nucleotides Shew_0215 and Shew_0216 are separated by 52 nucleotides Shew_0216 and Shew_0217 are separated by 74 nucleotides
Shew_0214: Shew_0214 - 3-dehydroquinate synthase (RefSeq), at 229,315 to 230,391
_0214
Shew_0215: Shew_0215 - sporulation domain-containing protein (RefSeq), at 230,408 to 231,943
_0215
Shew_0216: Shew_0216 - DNA adenine methylase (RefSeq), at 231,996 to 232,835
_0216
Shew_0217: Shew_0217 - hypothetical protein (RefSeq), at 232,910 to 233,083
_0217
Position (kb) 230
231
232 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 229.593 kb on - strand, within Shew_0214 at 229.767 kb on + strand, within Shew_0214 at 229.775 kb on - strand, within Shew_0214 at 229.827 kb on - strand, within Shew_0214 at 229.915 kb on - strand, within Shew_0214 at 229.991 kb on - strand, within Shew_0214 at 230.060 kb on - strand, within Shew_0214 at 230.522 kb on + strand at 230.579 kb on - strand, within Shew_0215 at 230.721 kb on + strand, within Shew_0215 at 230.769 kb on - strand, within Shew_0215 at 230.791 kb on + strand, within Shew_0215 at 230.937 kb on - strand, within Shew_0215 at 231.046 kb on - strand, within Shew_0215 at 231.287 kb on - strand, within Shew_0215 at 231.450 kb on + strand, within Shew_0215 at 231.463 kb on + strand, within Shew_0215 at 231.577 kb on + strand, within Shew_0215 at 231.580 kb on - strand, within Shew_0215 at 231.945 kb on + strand at 232.439 kb on + strand, within Shew_0216 at 232.497 kb on - strand, within Shew_0216 at 232.877 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 229,593 - Shew_0214 0.26 -0.7 229,767 + Shew_0214 0.42 -3.9 229,775 - Shew_0214 0.43 +0.2 229,827 - Shew_0214 0.48 +0.7 229,915 - Shew_0214 0.56 -1.0 229,991 - Shew_0214 0.63 -0.4 230,060 - Shew_0214 0.69 +1.4 230,522 + +0.4 230,579 - Shew_0215 0.11 +0.3 230,721 + Shew_0215 0.20 +0.2 230,769 - Shew_0215 0.24 +0.0 230,791 + Shew_0215 0.25 +1.0 230,937 - Shew_0215 0.34 +0.0 231,046 - Shew_0215 0.42 +0.0 231,287 - Shew_0215 0.57 +0.2 231,450 + Shew_0215 0.68 +0.3 231,463 + Shew_0215 0.69 +1.0 231,577 + Shew_0215 0.76 +0.1 231,580 - Shew_0215 0.76 -1.6 231,945 + +0.1 232,439 + Shew_0216 0.53 -0.4 232,497 - Shew_0216 0.60 +0.8 232,877 + -0.0
Or see this region's nucleotide sequence