Experiment: KB with Rifampicin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_RNA9 and Psyr_RNA10 are separated by 29 nucleotides Psyr_RNA10 and Psyr_RNA11 are separated by 5 nucleotides Psyr_RNA11 and Psyr_RNA12 are separated by 95 nucleotides Psyr_RNA12 and Psyr_0729 are separated by 326 nucleotides Psyr_0729 and Psyr_0730 overlap by 59 nucleotides Psyr_0730 and Psyr_0731 overlap by 4 nucleotides
Psyr_RNA9: Psyr_RNA9 - tRNA-Asn, at 835,185 to 835,260
_RNA9
Psyr_RNA10: Psyr_RNA10 - tRNA-Pro, at 835,290 to 835,366
_RNA10
Psyr_RNA11: Psyr_RNA11 - tRNA-Lys, at 835,372 to 835,447
_RNA11
Psyr_RNA12: Psyr_RNA12 - tRNA-Pro, at 835,543 to 835,619
_RNA12
Psyr_0729: Psyr_0729 - CDS, at 835,946 to 837,109
_0729
Psyr_0730: Psyr_0730 - CDS, at 837,051 to 837,347
_0730
Psyr_0731: Psyr_0731 - CDS, at 837,344 to 838,102
_0731
Position (kb)
835
836
837
838 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 834.959 kb on + strand at 834.959 kb on + strand at 834.959 kb on + strand at 834.959 kb on + strand at 834.959 kb on + strand at 835.021 kb on + strand at 835.085 kb on + strand at 835.085 kb on + strand at 835.128 kb on + strand at 835.160 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.421 kb on + strand, within Psyr_RNA11 at 835.736 kb on + strand at 835.736 kb on + strand at 835.859 kb on + strand at 835.872 kb on + strand at 835.872 kb on + strand at 835.872 kb on + strand at 835.876 kb on + strand at 835.903 kb on + strand at 835.903 kb on + strand at 835.918 kb on + strand at 835.930 kb on + strand at 835.930 kb on + strand at 835.995 kb on - strand at 835.995 kb on - strand at 835.995 kb on - strand at 836.050 kb on + strand at 836.050 kb on + strand at 836.067 kb on - strand at 836.067 kb on - strand, within Psyr_0729 at 836.067 kb on - strand, within Psyr_0729 at 836.067 kb on - strand, within Psyr_0729 at 836.141 kb on + strand, within Psyr_0729 at 836.201 kb on + strand, within Psyr_0729 at 836.210 kb on - strand, within Psyr_0729 at 836.248 kb on + strand, within Psyr_0729 at 836.249 kb on - strand, within Psyr_0729 at 836.369 kb on - strand, within Psyr_0729 at 836.696 kb on + strand, within Psyr_0729 at 836.741 kb on - strand, within Psyr_0729 at 836.847 kb on - strand, within Psyr_0729 at 836.938 kb on + strand, within Psyr_0729 at 836.938 kb on + strand, within Psyr_0729 at 837.065 kb on + strand at 837.065 kb on + strand at 837.065 kb on + strand at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.115 kb on + strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.116 kb on - strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.117 kb on + strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.118 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.140 kb on - strand, within Psyr_0730 at 837.593 kb on + strand, within Psyr_0731 at 837.593 kb on + strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.594 kb on - strand, within Psyr_0731 at 837.627 kb on - strand, within Psyr_0731 at 837.627 kb on - strand, within Psyr_0731 at 837.627 kb on - strand, within Psyr_0731 at 838.003 kb on + strand, within Psyr_0731 at 838.037 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rifampicin 0.1 mg/ml remove 834,959 + +0.9 834,959 + +0.7 834,959 + -0.6 834,959 + +0.6 834,959 + +1.3 835,021 + -0.1 835,085 + -2.2 835,085 + +0.0 835,128 + -2.6 835,160 + -1.0 835,367 + -0.8 835,367 + -0.7 835,367 + -0.7 835,367 + +0.6 835,367 + -1.6 835,421 + Psyr_RNA11 0.64 -1.2 835,736 + -0.8 835,736 + +1.3 835,859 + -0.7 835,872 + +0.4 835,872 + +0.2 835,872 + +0.6 835,876 + +0.1 835,903 + -0.9 835,903 + +0.3 835,918 + -0.2 835,930 + +0.6 835,930 + +0.2 835,995 - +0.8 835,995 - +0.1 835,995 - +0.6 836,050 + +0.6 836,050 + -0.6 836,067 - +2.2 836,067 - Psyr_0729 0.10 -1.6 836,067 - Psyr_0729 0.10 -0.4 836,067 - Psyr_0729 0.10 -2.2 836,141 + Psyr_0729 0.17 -1.0 836,201 + Psyr_0729 0.22 -1.0 836,210 - Psyr_0729 0.23 +0.1 836,248 + Psyr_0729 0.26 -0.7 836,249 - Psyr_0729 0.26 -0.6 836,369 - Psyr_0729 0.36 +0.3 836,696 + Psyr_0729 0.64 -0.3 836,741 - Psyr_0729 0.68 +0.7 836,847 - Psyr_0729 0.77 -1.3 836,938 + Psyr_0729 0.85 +0.3 836,938 + Psyr_0729 0.85 -1.3 837,065 + -0.9 837,065 + -0.2 837,065 + -0.7 837,115 + Psyr_0730 0.22 -0.6 837,115 + Psyr_0730 0.22 +1.2 837,115 + Psyr_0730 0.22 -2.2 837,115 + Psyr_0730 0.22 -0.2 837,115 + Psyr_0730 0.22 -1.5 837,115 + Psyr_0730 0.22 +1.3 837,115 + Psyr_0730 0.22 +1.4 837,115 + Psyr_0730 0.22 -0.3 837,115 + Psyr_0730 0.22 -2.2 837,115 + Psyr_0730 0.22 +2.9 837,115 + Psyr_0730 0.22 -0.1 837,115 + Psyr_0730 0.22 -1.2 837,115 + Psyr_0730 0.22 -0.4 837,116 - Psyr_0730 0.22 +0.2 837,116 - Psyr_0730 0.22 +0.1 837,116 - Psyr_0730 0.22 +0.5 837,116 - Psyr_0730 0.22 +1.9 837,116 - Psyr_0730 0.22 +1.1 837,116 - Psyr_0730 0.22 -0.1 837,116 - Psyr_0730 0.22 +0.4 837,116 - Psyr_0730 0.22 -0.6 837,116 - Psyr_0730 0.22 -0.2 837,116 - Psyr_0730 0.22 +0.6 837,116 - Psyr_0730 0.22 +0.5 837,116 - Psyr_0730 0.22 +0.4 837,116 - Psyr_0730 0.22 -0.4 837,116 - Psyr_0730 0.22 +1.2 837,116 - Psyr_0730 0.22 +0.6 837,116 - Psyr_0730 0.22 +0.3 837,116 - Psyr_0730 0.22 -0.3 837,117 + Psyr_0730 0.22 -0.3 837,117 + Psyr_0730 0.22 +0.9 837,117 + Psyr_0730 0.22 -0.2 837,117 + Psyr_0730 0.22 -0.1 837,117 + Psyr_0730 0.22 -1.7 837,118 - Psyr_0730 0.23 -0.0 837,118 - Psyr_0730 0.23 +2.0 837,118 - Psyr_0730 0.23 -0.7 837,118 - Psyr_0730 0.23 +0.5 837,118 - Psyr_0730 0.23 -0.6 837,118 - Psyr_0730 0.23 +0.0 837,118 - Psyr_0730 0.23 +0.2 837,118 - Psyr_0730 0.23 +2.0 837,118 - Psyr_0730 0.23 +0.8 837,118 - Psyr_0730 0.23 -0.2 837,118 - Psyr_0730 0.23 -0.5 837,118 - Psyr_0730 0.23 +1.0 837,140 - Psyr_0730 0.30 +0.4 837,140 - Psyr_0730 0.30 +1.3 837,140 - Psyr_0730 0.30 +0.9 837,140 - Psyr_0730 0.30 -0.1 837,140 - Psyr_0730 0.30 -0.0 837,593 + Psyr_0731 0.33 -0.1 837,593 + Psyr_0731 0.33 -0.1 837,594 - Psyr_0731 0.33 -0.5 837,594 - Psyr_0731 0.33 +0.1 837,594 - Psyr_0731 0.33 +0.3 837,594 - Psyr_0731 0.33 +0.8 837,594 - Psyr_0731 0.33 +0.3 837,594 - Psyr_0731 0.33 +0.3 837,594 - Psyr_0731 0.33 +2.2 837,627 - Psyr_0731 0.37 -0.5 837,627 - Psyr_0731 0.37 -0.5 837,627 - Psyr_0731 0.37 +0.8 838,003 + Psyr_0731 0.87 -0.1 838,037 - -2.2
Or see this region's nucleotide sequence