Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09165 and MMJJ_RS09170 overlap by 1 nucleotides MMJJ_RS09170 and MMJJ_RS09175 overlap by 8 nucleotides MMJJ_RS09175 and MMJJ_RS09180 overlap by 20 nucleotides MMJJ_RS09180 and MMJJ_RS09185 are separated by 10 nucleotides
MMJJ_RS09165: MMJJ_RS09165 - indole-3-glycerol phosphate synthase TrpC, at 1,701,173 to 1,701,862
_RS09165
MMJJ_RS09170: MMJJ_RS09170 - anthranilate phosphoribosyltransferase, at 1,701,862 to 1,702,827
_RS09170
MMJJ_RS09175: MMJJ_RS09175 - anthranilate synthase component I, at 1,702,820 to 1,704,139
_RS09175
MMJJ_RS09180: MMJJ_RS09180 - aminodeoxychorismate/anthranilate synthase component II, at 1,704,120 to 1,704,716
_RS09180
MMJJ_RS09185: MMJJ_RS09185 - phosphoribosylanthranilate isomerase, at 1,704,727 to 1,705,362
_RS09185
Position (kb) 1702
1703
1704
1705 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1701.961 kb on - strand at 1702.723 kb on + strand, within MMJJ_RS09170 at 1702.839 kb on - strand at 1703.893 kb on - strand, within MMJJ_RS09175 at 1703.986 kb on - strand, within MMJJ_RS09175 at 1704.492 kb on - strand, within MMJJ_RS09180
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,701,961 - +0.2 1,702,723 + MMJJ_RS09170 0.89 -1.8 1,702,839 - -1.6 1,703,893 - MMJJ_RS09175 0.81 -2.7 1,703,986 - MMJJ_RS09175 0.88 -4.0 1,704,492 - MMJJ_RS09180 0.62 -0.8
Or see this region's nucleotide sequence