Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08565 and MMJJ_RS08570 are separated by 126 nucleotides MMJJ_RS08570 and MMJJ_RS08575 are separated by 140 nucleotides MMJJ_RS08575 and MMJJ_RS08580 are separated by 4 nucleotides
MMJJ_RS08565: MMJJ_RS08565 - ATP-binding protein, at 1,576,757 to 1,578,601
_RS08565
MMJJ_RS08570: MMJJ_RS08570 - multidrug resistance efflux transporter family protein, at 1,578,728 to 1,579,672
_RS08570
MMJJ_RS08575: MMJJ_RS08575 - hypothetical protein, at 1,579,813 to 1,580,109
_RS08575
MMJJ_RS08580: MMJJ_RS08580 - YkgJ family cysteine cluster protein, at 1,580,114 to 1,580,611
_RS08580
Position (kb)
1578
1579
1580 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1577.738 kb on - strand, within MMJJ_RS08565 at 1577.783 kb on - strand, within MMJJ_RS08565 at 1577.787 kb on + strand, within MMJJ_RS08565 at 1577.787 kb on + strand, within MMJJ_RS08565 at 1577.800 kb on - strand, within MMJJ_RS08565 at 1577.890 kb on + strand, within MMJJ_RS08565 at 1577.890 kb on + strand, within MMJJ_RS08565 at 1577.891 kb on - strand, within MMJJ_RS08565 at 1577.891 kb on - strand, within MMJJ_RS08565 at 1577.891 kb on - strand, within MMJJ_RS08565 at 1577.892 kb on + strand, within MMJJ_RS08565 at 1577.892 kb on + strand, within MMJJ_RS08565 at 1577.893 kb on - strand, within MMJJ_RS08565 at 1577.893 kb on - strand, within MMJJ_RS08565 at 1577.893 kb on - strand, within MMJJ_RS08565 at 1577.947 kb on - strand, within MMJJ_RS08565 at 1577.948 kb on + strand, within MMJJ_RS08565 at 1577.951 kb on - strand, within MMJJ_RS08565 at 1577.952 kb on + strand, within MMJJ_RS08565 at 1577.957 kb on - strand, within MMJJ_RS08565 at 1577.958 kb on + strand, within MMJJ_RS08565 at 1577.966 kb on - strand, within MMJJ_RS08565 at 1577.966 kb on - strand, within MMJJ_RS08565 at 1577.971 kb on + strand, within MMJJ_RS08565 at 1577.971 kb on + strand, within MMJJ_RS08565 at 1577.971 kb on + strand, within MMJJ_RS08565 at 1577.972 kb on - strand, within MMJJ_RS08565 at 1578.002 kb on + strand, within MMJJ_RS08565 at 1578.028 kb on - strand, within MMJJ_RS08565 at 1578.028 kb on - strand, within MMJJ_RS08565 at 1578.036 kb on + strand, within MMJJ_RS08565 at 1578.036 kb on + strand, within MMJJ_RS08565 at 1578.037 kb on - strand, within MMJJ_RS08565 at 1578.041 kb on - strand, within MMJJ_RS08565 at 1578.041 kb on - strand, within MMJJ_RS08565 at 1578.041 kb on - strand, within MMJJ_RS08565 at 1578.042 kb on + strand, within MMJJ_RS08565 at 1578.045 kb on - strand, within MMJJ_RS08565 at 1578.046 kb on + strand, within MMJJ_RS08565 at 1578.059 kb on - strand, within MMJJ_RS08565 at 1578.059 kb on - strand, within MMJJ_RS08565 at 1578.061 kb on - strand, within MMJJ_RS08565 at 1578.062 kb on + strand, within MMJJ_RS08565 at 1578.078 kb on - strand, within MMJJ_RS08565 at 1578.078 kb on - strand, within MMJJ_RS08565 at 1578.079 kb on + strand, within MMJJ_RS08565 at 1578.079 kb on + strand, within MMJJ_RS08565 at 1578.081 kb on + strand, within MMJJ_RS08565 at 1578.081 kb on + strand, within MMJJ_RS08565 at 1578.081 kb on + strand, within MMJJ_RS08565 at 1578.136 kb on - strand, within MMJJ_RS08565 at 1578.177 kb on - strand, within MMJJ_RS08565 at 1578.177 kb on - strand, within MMJJ_RS08565 at 1578.177 kb on - strand, within MMJJ_RS08565 at 1578.178 kb on + strand, within MMJJ_RS08565 at 1578.178 kb on + strand, within MMJJ_RS08565 at 1578.178 kb on + strand, within MMJJ_RS08565 at 1578.178 kb on + strand, within MMJJ_RS08565 at 1578.179 kb on - strand, within MMJJ_RS08565 at 1578.179 kb on - strand, within MMJJ_RS08565 at 1578.179 kb on - strand, within MMJJ_RS08565 at 1578.179 kb on - strand, within MMJJ_RS08565 at 1578.179 kb on - strand, within MMJJ_RS08565 at 1578.206 kb on + strand, within MMJJ_RS08565 at 1578.210 kb on - strand, within MMJJ_RS08565 at 1578.211 kb on + strand, within MMJJ_RS08565 at 1578.211 kb on + strand, within MMJJ_RS08565 at 1578.211 kb on + strand, within MMJJ_RS08565 at 1578.211 kb on + strand, within MMJJ_RS08565 at 1578.254 kb on - strand, within MMJJ_RS08565 at 1578.255 kb on + strand, within MMJJ_RS08565 at 1578.341 kb on + strand, within MMJJ_RS08565 at 1578.352 kb on - strand, within MMJJ_RS08565 at 1578.385 kb on - strand, within MMJJ_RS08565 at 1578.387 kb on - strand, within MMJJ_RS08565 at 1578.438 kb on + strand at 1578.453 kb on + strand at 1578.455 kb on + strand at 1578.459 kb on + strand at 1578.459 kb on + strand at 1578.497 kb on + strand at 1578.497 kb on + strand at 1578.500 kb on + strand at 1578.510 kb on + strand at 1578.513 kb on - strand at 1578.513 kb on - strand at 1578.514 kb on + strand at 1578.536 kb on - strand at 1578.536 kb on - strand at 1578.545 kb on + strand at 1578.569 kb on + strand at 1578.645 kb on + strand at 1578.654 kb on - strand at 1578.658 kb on - strand at 1578.663 kb on - strand at 1578.668 kb on + strand at 1578.672 kb on + strand at 1578.676 kb on + strand at 1578.688 kb on + strand at 1578.688 kb on + strand at 1578.692 kb on + strand at 1578.737 kb on - strand at 1578.750 kb on - strand at 1578.751 kb on + strand at 1578.764 kb on - strand at 1578.765 kb on + strand at 1578.765 kb on + strand at 1578.777 kb on + strand at 1578.779 kb on - strand at 1578.830 kb on - strand, within MMJJ_RS08570 at 1578.831 kb on + strand, within MMJJ_RS08570 at 1578.857 kb on - strand, within MMJJ_RS08570 at 1578.860 kb on - strand, within MMJJ_RS08570 at 1578.861 kb on + strand, within MMJJ_RS08570 at 1578.914 kb on - strand, within MMJJ_RS08570 at 1578.915 kb on + strand, within MMJJ_RS08570 at 1578.976 kb on - strand, within MMJJ_RS08570 at 1578.976 kb on - strand, within MMJJ_RS08570 at 1579.182 kb on + strand, within MMJJ_RS08570 at 1579.310 kb on + strand, within MMJJ_RS08570 at 1579.385 kb on + strand, within MMJJ_RS08570 at 1579.428 kb on - strand, within MMJJ_RS08570 at 1579.429 kb on + strand, within MMJJ_RS08570 at 1579.433 kb on - strand, within MMJJ_RS08570 at 1579.433 kb on - strand, within MMJJ_RS08570 at 1579.450 kb on - strand, within MMJJ_RS08570 at 1579.451 kb on + strand, within MMJJ_RS08570 at 1579.451 kb on + strand, within MMJJ_RS08570 at 1579.451 kb on + strand, within MMJJ_RS08570 at 1579.526 kb on - strand, within MMJJ_RS08570 at 1579.527 kb on + strand, within MMJJ_RS08570 at 1579.527 kb on + strand, within MMJJ_RS08570 at 1579.553 kb on - strand, within MMJJ_RS08570 at 1579.553 kb on - strand, within MMJJ_RS08570 at 1579.553 kb on - strand, within MMJJ_RS08570 at 1579.554 kb on + strand, within MMJJ_RS08570 at 1579.554 kb on + strand, within MMJJ_RS08570 at 1579.557 kb on - strand, within MMJJ_RS08570 at 1579.557 kb on - strand, within MMJJ_RS08570 at 1579.557 kb on - strand, within MMJJ_RS08570 at 1579.557 kb on - strand, within MMJJ_RS08570 at 1579.558 kb on + strand, within MMJJ_RS08570 at 1579.558 kb on + strand, within MMJJ_RS08570 at 1579.558 kb on + strand, within MMJJ_RS08570 at 1579.558 kb on + strand, within MMJJ_RS08570 at 1579.624 kb on - strand at 1579.624 kb on - strand at 1579.624 kb on - strand at 1579.624 kb on - strand at 1579.625 kb on + strand at 1579.625 kb on + strand at 1579.684 kb on + strand at 1579.685 kb on - strand at 1579.685 kb on - strand at 1579.686 kb on + strand at 1579.686 kb on + strand at 1579.686 kb on + strand at 1579.686 kb on + strand at 1579.686 kb on + strand at 1579.686 kb on + strand at 1579.686 kb on + strand at 1579.689 kb on - strand at 1579.702 kb on + strand at 1579.702 kb on + strand at 1579.732 kb on - strand at 1579.733 kb on + strand at 1579.733 kb on + strand at 1579.738 kb on - strand at 1579.740 kb on - strand at 1579.740 kb on - strand at 1579.741 kb on + strand at 1579.741 kb on + strand at 1579.743 kb on - strand at 1579.743 kb on - strand at 1579.743 kb on - strand at 1579.748 kb on + strand at 1579.748 kb on + strand at 1579.759 kb on + strand at 1579.767 kb on + strand at 1579.767 kb on + strand at 1579.769 kb on - strand at 1579.772 kb on + strand at 1579.826 kb on - strand at 1579.826 kb on - strand at 1579.826 kb on - strand at 1579.826 kb on - strand at 1579.827 kb on + strand at 1579.827 kb on + strand at 1579.909 kb on + strand, within MMJJ_RS08575 at 1579.929 kb on - strand, within MMJJ_RS08575 at 1579.930 kb on + strand, within MMJJ_RS08575 at 1579.977 kb on - strand, within MMJJ_RS08575 at 1580.026 kb on - strand, within MMJJ_RS08575 at 1580.026 kb on - strand, within MMJJ_RS08575 at 1580.065 kb on + strand, within MMJJ_RS08575 at 1580.115 kb on - strand at 1580.180 kb on + strand, within MMJJ_RS08580 at 1580.224 kb on - strand, within MMJJ_RS08580 at 1580.224 kb on - strand, within MMJJ_RS08580 at 1580.227 kb on + strand, within MMJJ_RS08580 at 1580.230 kb on + strand, within MMJJ_RS08580 at 1580.270 kb on + strand, within MMJJ_RS08580 at 1580.335 kb on + strand, within MMJJ_RS08580 at 1580.354 kb on + strand, within MMJJ_RS08580 at 1580.383 kb on - strand, within MMJJ_RS08580 at 1580.384 kb on + strand, within MMJJ_RS08580 at 1580.507 kb on + strand, within MMJJ_RS08580 at 1580.657 kb on + strand at 1580.662 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 1,577,738 - MMJJ_RS08565 0.53 -0.3 1,577,783 - MMJJ_RS08565 0.56 -0.4 1,577,787 + MMJJ_RS08565 0.56 +0.1 1,577,787 + MMJJ_RS08565 0.56 -1.0 1,577,800 - MMJJ_RS08565 0.57 +0.9 1,577,890 + MMJJ_RS08565 0.61 +0.8 1,577,890 + MMJJ_RS08565 0.61 +0.2 1,577,891 - MMJJ_RS08565 0.61 +1.0 1,577,891 - MMJJ_RS08565 0.61 -2.8 1,577,891 - MMJJ_RS08565 0.61 +1.7 1,577,892 + MMJJ_RS08565 0.62 +1.3 1,577,892 + MMJJ_RS08565 0.62 -0.1 1,577,893 - MMJJ_RS08565 0.62 +0.5 1,577,893 - MMJJ_RS08565 0.62 +0.8 1,577,893 - MMJJ_RS08565 0.62 -0.8 1,577,947 - MMJJ_RS08565 0.64 -1.5 1,577,948 + MMJJ_RS08565 0.65 +0.5 1,577,951 - MMJJ_RS08565 0.65 +0.4 1,577,952 + MMJJ_RS08565 0.65 +0.3 1,577,957 - MMJJ_RS08565 0.65 -0.3 1,577,958 + MMJJ_RS08565 0.65 +0.3 1,577,966 - MMJJ_RS08565 0.66 +0.5 1,577,966 - MMJJ_RS08565 0.66 +0.7 1,577,971 + MMJJ_RS08565 0.66 -2.4 1,577,971 + MMJJ_RS08565 0.66 +0.0 1,577,971 + MMJJ_RS08565 0.66 +1.2 1,577,972 - MMJJ_RS08565 0.66 +0.4 1,578,002 + MMJJ_RS08565 0.67 -0.2 1,578,028 - MMJJ_RS08565 0.69 +0.3 1,578,028 - MMJJ_RS08565 0.69 +0.1 1,578,036 + MMJJ_RS08565 0.69 +0.4 1,578,036 + MMJJ_RS08565 0.69 +0.0 1,578,037 - MMJJ_RS08565 0.69 +1.2 1,578,041 - MMJJ_RS08565 0.70 -0.6 1,578,041 - MMJJ_RS08565 0.70 -0.4 1,578,041 - MMJJ_RS08565 0.70 +0.4 1,578,042 + MMJJ_RS08565 0.70 +0.1 1,578,045 - MMJJ_RS08565 0.70 +0.3 1,578,046 + MMJJ_RS08565 0.70 +1.4 1,578,059 - MMJJ_RS08565 0.71 -1.1 1,578,059 - MMJJ_RS08565 0.71 -0.6 1,578,061 - MMJJ_RS08565 0.71 +0.8 1,578,062 + MMJJ_RS08565 0.71 +0.4 1,578,078 - MMJJ_RS08565 0.72 -0.8 1,578,078 - MMJJ_RS08565 0.72 -0.6 1,578,079 + MMJJ_RS08565 0.72 -2.1 1,578,079 + MMJJ_RS08565 0.72 +0.6 1,578,081 + MMJJ_RS08565 0.72 +0.1 1,578,081 + MMJJ_RS08565 0.72 +0.4 1,578,081 + MMJJ_RS08565 0.72 +1.1 1,578,136 - MMJJ_RS08565 0.75 +0.5 1,578,177 - MMJJ_RS08565 0.77 +0.1 1,578,177 - MMJJ_RS08565 0.77 +0.9 1,578,177 - MMJJ_RS08565 0.77 +0.9 1,578,178 + MMJJ_RS08565 0.77 -0.9 1,578,178 + MMJJ_RS08565 0.77 +0.5 1,578,178 + MMJJ_RS08565 0.77 +0.3 1,578,178 + MMJJ_RS08565 0.77 +0.8 1,578,179 - MMJJ_RS08565 0.77 +0.3 1,578,179 - MMJJ_RS08565 0.77 +0.3 1,578,179 - MMJJ_RS08565 0.77 +0.4 1,578,179 - MMJJ_RS08565 0.77 +0.0 1,578,179 - MMJJ_RS08565 0.77 +1.0 1,578,206 + MMJJ_RS08565 0.79 +0.5 1,578,210 - MMJJ_RS08565 0.79 +0.5 1,578,211 + MMJJ_RS08565 0.79 -1.0 1,578,211 + MMJJ_RS08565 0.79 +0.7 1,578,211 + MMJJ_RS08565 0.79 +0.6 1,578,211 + MMJJ_RS08565 0.79 +0.7 1,578,254 - MMJJ_RS08565 0.81 -0.4 1,578,255 + MMJJ_RS08565 0.81 +0.6 1,578,341 + MMJJ_RS08565 0.86 +0.0 1,578,352 - MMJJ_RS08565 0.86 -0.9 1,578,385 - MMJJ_RS08565 0.88 -1.8 1,578,387 - MMJJ_RS08565 0.88 +0.3 1,578,438 + +0.5 1,578,453 + +0.0 1,578,455 + +1.0 1,578,459 + -0.9 1,578,459 + +0.7 1,578,497 + +0.8 1,578,497 + +0.6 1,578,500 + -1.6 1,578,510 + +0.8 1,578,513 - +0.6 1,578,513 - +0.4 1,578,514 + -0.6 1,578,536 - -0.9 1,578,536 - +0.1 1,578,545 + +0.7 1,578,569 + +0.7 1,578,645 + -0.8 1,578,654 - -1.3 1,578,658 - +0.0 1,578,663 - +1.1 1,578,668 + -1.9 1,578,672 + +0.8 1,578,676 + -0.0 1,578,688 + +0.3 1,578,688 + +0.6 1,578,692 + +1.0 1,578,737 - +0.5 1,578,750 - +0.4 1,578,751 + +0.1 1,578,764 - +1.4 1,578,765 + -1.7 1,578,765 + -1.6 1,578,777 + +1.1 1,578,779 - +0.9 1,578,830 - MMJJ_RS08570 0.11 +1.4 1,578,831 + MMJJ_RS08570 0.11 +0.4 1,578,857 - MMJJ_RS08570 0.14 +0.2 1,578,860 - MMJJ_RS08570 0.14 +0.8 1,578,861 + MMJJ_RS08570 0.14 +0.1 1,578,914 - MMJJ_RS08570 0.20 -1.9 1,578,915 + MMJJ_RS08570 0.20 -0.5 1,578,976 - MMJJ_RS08570 0.26 +0.9 1,578,976 - MMJJ_RS08570 0.26 -0.3 1,579,182 + MMJJ_RS08570 0.48 -0.4 1,579,310 + MMJJ_RS08570 0.62 +1.1 1,579,385 + MMJJ_RS08570 0.70 +0.0 1,579,428 - MMJJ_RS08570 0.74 +1.2 1,579,429 + MMJJ_RS08570 0.74 -0.3 1,579,433 - MMJJ_RS08570 0.75 -0.3 1,579,433 - MMJJ_RS08570 0.75 -1.7 1,579,450 - MMJJ_RS08570 0.76 -0.3 1,579,451 + MMJJ_RS08570 0.77 -0.1 1,579,451 + MMJJ_RS08570 0.77 -2.4 1,579,451 + MMJJ_RS08570 0.77 +0.4 1,579,526 - MMJJ_RS08570 0.84 -0.6 1,579,527 + MMJJ_RS08570 0.85 +0.1 1,579,527 + MMJJ_RS08570 0.85 -0.8 1,579,553 - MMJJ_RS08570 0.87 -0.2 1,579,553 - MMJJ_RS08570 0.87 +0.3 1,579,553 - MMJJ_RS08570 0.87 -0.2 1,579,554 + MMJJ_RS08570 0.87 +0.2 1,579,554 + MMJJ_RS08570 0.87 +0.7 1,579,557 - MMJJ_RS08570 0.88 +3.9 1,579,557 - MMJJ_RS08570 0.88 +0.6 1,579,557 - MMJJ_RS08570 0.88 -0.6 1,579,557 - MMJJ_RS08570 0.88 +0.3 1,579,558 + MMJJ_RS08570 0.88 +1.0 1,579,558 + MMJJ_RS08570 0.88 -1.4 1,579,558 + MMJJ_RS08570 0.88 -0.6 1,579,558 + MMJJ_RS08570 0.88 +0.5 1,579,624 - -0.5 1,579,624 - -0.7 1,579,624 - +0.8 1,579,624 - +0.7 1,579,625 + -0.6 1,579,625 + +0.1 1,579,684 + +0.8 1,579,685 - -0.2 1,579,685 - -1.6 1,579,686 + +0.8 1,579,686 + -0.7 1,579,686 + -0.2 1,579,686 + +1.5 1,579,686 + -0.7 1,579,686 + +0.4 1,579,686 + -0.0 1,579,689 - +0.0 1,579,702 + -1.1 1,579,702 + -0.4 1,579,732 - -0.8 1,579,733 + -1.6 1,579,733 + +0.0 1,579,738 - +0.8 1,579,740 - -0.3 1,579,740 - +1.2 1,579,741 + -1.8 1,579,741 + -2.2 1,579,743 - -1.3 1,579,743 - -0.6 1,579,743 - -4.3 1,579,748 + -0.6 1,579,748 + +1.0 1,579,759 + +0.9 1,579,767 + -0.0 1,579,767 + -1.5 1,579,769 - +0.2 1,579,772 + +0.6 1,579,826 - -2.9 1,579,826 - -1.6 1,579,826 - -1.8 1,579,826 - +1.1 1,579,827 + -2.7 1,579,827 + -2.4 1,579,909 + MMJJ_RS08575 0.32 -2.2 1,579,929 - MMJJ_RS08575 0.39 -0.8 1,579,930 + MMJJ_RS08575 0.39 -2.8 1,579,977 - MMJJ_RS08575 0.55 -2.3 1,580,026 - MMJJ_RS08575 0.72 -1.0 1,580,026 - MMJJ_RS08575 0.72 -2.9 1,580,065 + MMJJ_RS08575 0.85 -2.4 1,580,115 - +0.9 1,580,180 + MMJJ_RS08580 0.13 -1.4 1,580,224 - MMJJ_RS08580 0.22 +0.2 1,580,224 - MMJJ_RS08580 0.22 -1.2 1,580,227 + MMJJ_RS08580 0.23 +0.9 1,580,230 + MMJJ_RS08580 0.23 -0.7 1,580,270 + MMJJ_RS08580 0.31 -1.1 1,580,335 + MMJJ_RS08580 0.44 -0.3 1,580,354 + MMJJ_RS08580 0.48 -0.0 1,580,383 - MMJJ_RS08580 0.54 -0.2 1,580,384 + MMJJ_RS08580 0.54 -1.2 1,580,507 + MMJJ_RS08580 0.79 -0.5 1,580,657 + -0.5 1,580,662 + +0.4
Or see this region's nucleotide sequence