Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS06065

Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS06060 and MMJJ_RS06065 are separated by 86 nucleotidesMMJJ_RS06065 and MMJJ_RS06070 are separated by 69 nucleotides MMJJ_RS06060: MMJJ_RS06060 - aspartate--tRNA(Asn) ligase, at 1,125,780 to 1,127,096 _RS06060 MMJJ_RS06065: MMJJ_RS06065 - metal-sulfur cluster assembly factor, at 1,127,183 to 1,127,482 _RS06065 MMJJ_RS06070: MMJJ_RS06070 - histidine--tRNA ligase, at 1,127,552 to 1,128,802 _RS06070 Position (kb) 1127 1128Strain fitness (log2 ratio) -2 -1 0 1at 1126.289 kb on + strand, within MMJJ_RS06060at 1127.119 kb on - strandat 1127.122 kb on - strandat 1127.122 kb on - strandat 1127.125 kb on - strandat 1127.133 kb on - strandat 1127.192 kb on + strandat 1127.223 kb on - strand, within MMJJ_RS06065at 1127.266 kb on - strand, within MMJJ_RS06065at 1127.314 kb on + strand, within MMJJ_RS06065at 1127.443 kb on + strandat 1127.504 kb on - strandat 1127.506 kb on - strandat 1127.507 kb on + strandat 1128.351 kb on + strand, within MMJJ_RS06070at 1128.353 kb on - strand, within MMJJ_RS06070

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride
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1,126,289 + MMJJ_RS06060 0.39 -1.2
1,127,119 - +0.2
1,127,122 - +0.2
1,127,122 - +0.1
1,127,125 - -1.9
1,127,133 - -0.6
1,127,192 + -1.0
1,127,223 - MMJJ_RS06065 0.13 -2.0
1,127,266 - MMJJ_RS06065 0.28 -1.8
1,127,314 + MMJJ_RS06065 0.44 -1.0
1,127,443 + -0.6
1,127,504 - -1.4
1,127,506 - -1.4
1,127,507 + +1.4
1,128,351 + MMJJ_RS06070 0.64 -0.6
1,128,353 - MMJJ_RS06070 0.64 -1.0

Or see this region's nucleotide sequence