Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03795 and MMJJ_RS03800 are separated by 158 nucleotides MMJJ_RS03800 and MMJJ_RS03805 are separated by 11 nucleotides MMJJ_RS03805 and MMJJ_RS03810 overlap by 4 nucleotides
MMJJ_RS03795: MMJJ_RS03795 - PQQ-binding-like beta-propeller repeat protein, at 706,495 to 707,463
_RS03795
MMJJ_RS03800: MMJJ_RS03800 - aldolase, at 707,622 to 708,548
_RS03800
MMJJ_RS03805: MMJJ_RS03805 - hypothetical protein, at 708,560 to 708,745
_RS03805
MMJJ_RS03810: MMJJ_RS03810 - AIR synthase-related protein, at 708,742 to 710,085
_RS03810
Position (kb) 707
708
709 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 706.666 kb on + strand, within MMJJ_RS03795 at 706.754 kb on + strand, within MMJJ_RS03795 at 706.775 kb on + strand, within MMJJ_RS03795 at 706.779 kb on + strand, within MMJJ_RS03795 at 706.779 kb on + strand, within MMJJ_RS03795 at 706.813 kb on + strand, within MMJJ_RS03795 at 706.893 kb on + strand, within MMJJ_RS03795 at 706.986 kb on + strand, within MMJJ_RS03795 at 706.986 kb on + strand, within MMJJ_RS03795 at 707.036 kb on + strand, within MMJJ_RS03795 at 707.048 kb on - strand, within MMJJ_RS03795 at 707.048 kb on - strand, within MMJJ_RS03795 at 707.049 kb on + strand, within MMJJ_RS03795 at 707.049 kb on + strand, within MMJJ_RS03795 at 707.049 kb on + strand, within MMJJ_RS03795 at 707.181 kb on - strand, within MMJJ_RS03795 at 707.274 kb on - strand, within MMJJ_RS03795 at 707.274 kb on - strand, within MMJJ_RS03795 at 707.275 kb on + strand, within MMJJ_RS03795 at 707.284 kb on - strand, within MMJJ_RS03795 at 707.326 kb on + strand, within MMJJ_RS03795 at 707.363 kb on - strand, within MMJJ_RS03795 at 707.363 kb on - strand, within MMJJ_RS03795 at 707.363 kb on - strand, within MMJJ_RS03795 at 707.363 kb on - strand, within MMJJ_RS03795 at 707.364 kb on + strand, within MMJJ_RS03795 at 707.364 kb on + strand, within MMJJ_RS03795 at 707.364 kb on + strand, within MMJJ_RS03795 at 707.364 kb on + strand, within MMJJ_RS03795 at 707.364 kb on + strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.365 kb on - strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.366 kb on + strand, within MMJJ_RS03795 at 707.379 kb on - strand at 707.406 kb on - strand at 707.407 kb on + strand at 707.407 kb on + strand at 707.497 kb on - strand at 707.497 kb on - strand at 707.497 kb on - strand at 707.497 kb on - strand at 707.498 kb on + strand at 707.503 kb on - strand at 707.503 kb on - strand at 707.504 kb on + strand at 707.504 kb on + strand at 707.504 kb on + strand at 707.505 kb on - strand at 707.505 kb on - strand at 707.505 kb on - strand at 707.506 kb on + strand at 707.506 kb on + strand at 707.511 kb on - strand at 707.511 kb on - strand at 707.511 kb on - strand at 707.511 kb on - strand at 707.512 kb on + strand at 707.512 kb on + strand at 707.512 kb on + strand at 707.512 kb on + strand at 707.512 kb on + strand at 707.512 kb on + strand at 707.516 kb on - strand at 707.517 kb on + strand at 707.517 kb on + strand at 707.518 kb on - strand at 707.518 kb on - strand at 707.518 kb on - strand at 707.518 kb on - strand at 707.518 kb on - strand at 707.519 kb on + strand at 707.519 kb on + strand at 707.519 kb on + strand at 707.519 kb on + strand at 707.519 kb on + strand at 707.519 kb on + strand at 707.520 kb on - strand at 707.520 kb on - strand at 707.520 kb on - strand at 707.520 kb on - strand at 707.520 kb on - strand at 707.521 kb on + strand at 707.521 kb on + strand at 707.521 kb on + strand at 707.523 kb on - strand at 707.543 kb on - strand at 707.543 kb on - strand at 707.549 kb on - strand at 707.554 kb on - strand at 707.559 kb on - strand at 707.571 kb on - strand at 707.572 kb on + strand at 707.579 kb on - strand at 707.579 kb on - strand at 707.579 kb on - strand at 707.580 kb on + strand at 707.592 kb on + strand at 707.595 kb on - strand at 707.600 kb on + strand at 707.606 kb on - strand at 707.635 kb on - strand at 707.651 kb on + strand at 707.653 kb on - strand at 707.692 kb on + strand at 707.724 kb on + strand, within MMJJ_RS03800 at 707.770 kb on - strand, within MMJJ_RS03800 at 707.913 kb on + strand, within MMJJ_RS03800 at 707.914 kb on - strand, within MMJJ_RS03800 at 707.920 kb on + strand, within MMJJ_RS03800 at 708.000 kb on + strand, within MMJJ_RS03800 at 708.005 kb on - strand, within MMJJ_RS03800 at 708.006 kb on + strand, within MMJJ_RS03800 at 708.006 kb on + strand, within MMJJ_RS03800 at 708.007 kb on - strand, within MMJJ_RS03800 at 708.010 kb on - strand, within MMJJ_RS03800 at 708.056 kb on - strand, within MMJJ_RS03800 at 708.056 kb on - strand, within MMJJ_RS03800 at 708.057 kb on + strand, within MMJJ_RS03800 at 708.057 kb on + strand, within MMJJ_RS03800 at 708.061 kb on - strand, within MMJJ_RS03800 at 708.061 kb on - strand, within MMJJ_RS03800 at 708.061 kb on - strand, within MMJJ_RS03800 at 708.061 kb on - strand, within MMJJ_RS03800 at 708.062 kb on + strand, within MMJJ_RS03800 at 708.062 kb on + strand, within MMJJ_RS03800 at 708.062 kb on + strand, within MMJJ_RS03800 at 708.062 kb on + strand, within MMJJ_RS03800 at 708.063 kb on - strand, within MMJJ_RS03800 at 708.064 kb on + strand, within MMJJ_RS03800 at 708.078 kb on - strand, within MMJJ_RS03800 at 708.117 kb on - strand, within MMJJ_RS03800 at 708.146 kb on + strand, within MMJJ_RS03800 at 708.146 kb on + strand, within MMJJ_RS03800 at 708.146 kb on + strand, within MMJJ_RS03800 at 708.216 kb on + strand, within MMJJ_RS03800 at 708.257 kb on + strand, within MMJJ_RS03800 at 708.293 kb on - strand, within MMJJ_RS03800 at 708.294 kb on + strand, within MMJJ_RS03800 at 708.294 kb on + strand, within MMJJ_RS03800 at 708.323 kb on - strand, within MMJJ_RS03800 at 708.324 kb on + strand, within MMJJ_RS03800 at 708.332 kb on + strand, within MMJJ_RS03800 at 708.488 kb on + strand at 708.524 kb on - strand at 708.525 kb on + strand at 708.525 kb on + strand at 708.525 kb on + strand at 708.526 kb on - strand at 708.529 kb on + strand at 708.556 kb on - strand at 708.557 kb on + strand at 708.576 kb on - strand at 708.577 kb on + strand at 708.587 kb on + strand, within MMJJ_RS03805 at 708.675 kb on - strand, within MMJJ_RS03805 at 708.681 kb on - strand, within MMJJ_RS03805 at 708.682 kb on + strand, within MMJJ_RS03805 at 708.682 kb on + strand, within MMJJ_RS03805 at 708.718 kb on + strand, within MMJJ_RS03805 at 708.728 kb on + strand at 708.728 kb on + strand at 708.728 kb on + strand at 708.730 kb on - strand at 708.731 kb on + strand at 708.734 kb on + strand at 708.763 kb on - strand at 708.763 kb on - strand at 708.764 kb on + strand at 708.764 kb on + strand at 708.766 kb on - strand at 708.931 kb on - strand, within MMJJ_RS03810 at 709.268 kb on + strand, within MMJJ_RS03810 at 709.279 kb on - strand, within MMJJ_RS03810 at 709.473 kb on - strand, within MMJJ_RS03810
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 706,666 + MMJJ_RS03795 0.18 +0.2 706,754 + MMJJ_RS03795 0.27 +1.2 706,775 + MMJJ_RS03795 0.29 +0.1 706,779 + MMJJ_RS03795 0.29 -1.7 706,779 + MMJJ_RS03795 0.29 -0.5 706,813 + MMJJ_RS03795 0.33 +0.7 706,893 + MMJJ_RS03795 0.41 +1.2 706,986 + MMJJ_RS03795 0.51 +0.7 706,986 + MMJJ_RS03795 0.51 -0.1 707,036 + MMJJ_RS03795 0.56 +0.2 707,048 - MMJJ_RS03795 0.57 -0.8 707,048 - MMJJ_RS03795 0.57 -0.1 707,049 + MMJJ_RS03795 0.57 +0.7 707,049 + MMJJ_RS03795 0.57 +0.4 707,049 + MMJJ_RS03795 0.57 -0.0 707,181 - MMJJ_RS03795 0.71 -0.3 707,274 - MMJJ_RS03795 0.80 +0.2 707,274 - MMJJ_RS03795 0.80 +0.0 707,275 + MMJJ_RS03795 0.80 +0.3 707,284 - MMJJ_RS03795 0.81 -0.1 707,326 + MMJJ_RS03795 0.86 -2.9 707,363 - MMJJ_RS03795 0.90 -0.1 707,363 - MMJJ_RS03795 0.90 +1.3 707,363 - MMJJ_RS03795 0.90 -0.5 707,363 - MMJJ_RS03795 0.90 -1.3 707,364 + MMJJ_RS03795 0.90 -1.1 707,364 + MMJJ_RS03795 0.90 +0.4 707,364 + MMJJ_RS03795 0.90 +0.6 707,364 + MMJJ_RS03795 0.90 -0.5 707,364 + MMJJ_RS03795 0.90 -0.3 707,365 - MMJJ_RS03795 0.90 -1.0 707,365 - MMJJ_RS03795 0.90 +0.7 707,365 - MMJJ_RS03795 0.90 +0.8 707,365 - MMJJ_RS03795 0.90 -0.7 707,365 - MMJJ_RS03795 0.90 -0.3 707,365 - MMJJ_RS03795 0.90 -0.4 707,365 - MMJJ_RS03795 0.90 -0.8 707,365 - MMJJ_RS03795 0.90 +0.4 707,365 - MMJJ_RS03795 0.90 -0.6 707,365 - MMJJ_RS03795 0.90 -0.1 707,365 - MMJJ_RS03795 0.90 -1.3 707,365 - MMJJ_RS03795 0.90 +1.5 707,365 - MMJJ_RS03795 0.90 +0.6 707,366 + MMJJ_RS03795 0.90 +1.1 707,366 + MMJJ_RS03795 0.90 -0.0 707,366 + MMJJ_RS03795 0.90 -2.0 707,366 + MMJJ_RS03795 0.90 +0.1 707,366 + MMJJ_RS03795 0.90 -1.0 707,366 + MMJJ_RS03795 0.90 -0.9 707,366 + MMJJ_RS03795 0.90 +0.3 707,366 + MMJJ_RS03795 0.90 +0.2 707,366 + MMJJ_RS03795 0.90 -0.7 707,366 + MMJJ_RS03795 0.90 +0.9 707,366 + MMJJ_RS03795 0.90 -0.7 707,379 - -1.3 707,406 - -1.9 707,407 + +1.2 707,407 + +0.6 707,497 - -0.7 707,497 - -0.7 707,497 - +0.5 707,497 - -0.4 707,498 + +0.0 707,503 - +0.0 707,503 - +0.7 707,504 + +0.1 707,504 + +0.4 707,504 + +1.0 707,505 - -0.3 707,505 - -1.8 707,505 - -1.3 707,506 + +0.4 707,506 + +0.6 707,511 - +1.0 707,511 - -0.9 707,511 - -0.8 707,511 - +0.7 707,512 + -1.8 707,512 + +0.8 707,512 + +0.4 707,512 + +0.3 707,512 + -0.4 707,512 + -3.1 707,516 - +0.3 707,517 + -0.3 707,517 + -0.3 707,518 - -0.0 707,518 - -0.9 707,518 - +0.7 707,518 - -1.1 707,518 - +1.5 707,519 + -0.3 707,519 + -1.0 707,519 + +1.0 707,519 + -0.5 707,519 + +0.5 707,519 + +0.4 707,520 - +0.9 707,520 - +0.8 707,520 - +0.8 707,520 - -1.2 707,520 - +0.9 707,521 + +0.6 707,521 + +0.9 707,521 + -0.1 707,523 - -0.1 707,543 - +0.0 707,543 - -1.9 707,549 - +0.3 707,554 - -2.3 707,559 - +1.0 707,571 - -2.6 707,572 + -0.3 707,579 - -1.9 707,579 - -2.4 707,579 - -1.3 707,580 + -0.7 707,592 + -1.8 707,595 - -2.3 707,600 + -0.8 707,606 - -1.2 707,635 - -3.8 707,651 + -4.5 707,653 - -1.1 707,692 + -3.2 707,724 + MMJJ_RS03800 0.11 -2.4 707,770 - MMJJ_RS03800 0.16 -4.1 707,913 + MMJJ_RS03800 0.31 -2.2 707,914 - MMJJ_RS03800 0.31 -1.7 707,920 + MMJJ_RS03800 0.32 -4.9 708,000 + MMJJ_RS03800 0.41 -1.1 708,005 - MMJJ_RS03800 0.41 -1.4 708,006 + MMJJ_RS03800 0.41 -4.8 708,006 + MMJJ_RS03800 0.41 -4.4 708,007 - MMJJ_RS03800 0.42 -3.6 708,010 - MMJJ_RS03800 0.42 -1.4 708,056 - MMJJ_RS03800 0.47 -2.1 708,056 - MMJJ_RS03800 0.47 -1.8 708,057 + MMJJ_RS03800 0.47 -1.5 708,057 + MMJJ_RS03800 0.47 -0.4 708,061 - MMJJ_RS03800 0.47 -4.0 708,061 - MMJJ_RS03800 0.47 -1.4 708,061 - MMJJ_RS03800 0.47 -3.1 708,061 - MMJJ_RS03800 0.47 -0.3 708,062 + MMJJ_RS03800 0.47 -4.0 708,062 + MMJJ_RS03800 0.47 -3.4 708,062 + MMJJ_RS03800 0.47 -5.1 708,062 + MMJJ_RS03800 0.47 -2.1 708,063 - MMJJ_RS03800 0.48 -4.1 708,064 + MMJJ_RS03800 0.48 -2.3 708,078 - MMJJ_RS03800 0.49 -5.4 708,117 - MMJJ_RS03800 0.53 -4.7 708,146 + MMJJ_RS03800 0.57 -1.2 708,146 + MMJJ_RS03800 0.57 -2.4 708,146 + MMJJ_RS03800 0.57 -3.8 708,216 + MMJJ_RS03800 0.64 -3.3 708,257 + MMJJ_RS03800 0.69 -4.3 708,293 - MMJJ_RS03800 0.72 -2.5 708,294 + MMJJ_RS03800 0.72 -2.1 708,294 + MMJJ_RS03800 0.72 -4.5 708,323 - MMJJ_RS03800 0.76 -1.9 708,324 + MMJJ_RS03800 0.76 -3.8 708,332 + MMJJ_RS03800 0.77 -5.0 708,488 + +0.4 708,524 - -1.2 708,525 + -4.4 708,525 + -2.5 708,525 + -2.4 708,526 - -1.4 708,529 + -2.5 708,556 - -0.0 708,557 + +1.2 708,576 - -0.3 708,577 + -0.3 708,587 + MMJJ_RS03805 0.15 -0.4 708,675 - MMJJ_RS03805 0.62 +1.0 708,681 - MMJJ_RS03805 0.65 -0.8 708,682 + MMJJ_RS03805 0.66 -1.3 708,682 + MMJJ_RS03805 0.66 +0.8 708,718 + MMJJ_RS03805 0.85 +1.1 708,728 + -0.8 708,728 + -0.3 708,728 + +1.5 708,730 - +0.8 708,731 + -0.6 708,734 + +1.0 708,763 - +0.2 708,763 - -0.7 708,764 + -1.7 708,764 + -0.7 708,766 - -0.5 708,931 - MMJJ_RS03810 0.14 -1.8 709,268 + MMJJ_RS03810 0.39 -2.5 709,279 - MMJJ_RS03810 0.40 +1.0 709,473 - MMJJ_RS03810 0.54 -2.3
Or see this region's nucleotide sequence