Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00230 and MMJJ_RS00235 are separated by 73 nucleotides MMJJ_RS00235 and MMJJ_RS00240 are separated by 88 nucleotides
MMJJ_RS00230: MMJJ_RS00230 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB, at 44,099 to 45,508
_RS00230
MMJJ_RS00235: MMJJ_RS00235 - hypothetical protein, at 45,582 to 47,423
_RS00235
MMJJ_RS00240: MMJJ_RS00240 - AI-2E family transporter, at 47,512 to 48,522
_RS00240
Position (kb) 45
46
47
48 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 45.483 kb on - strand at 45.506 kb on - strand at 45.532 kb on + strand at 45.564 kb on + strand at 45.609 kb on - strand at 45.642 kb on - strand at 45.646 kb on - strand at 45.646 kb on - strand at 45.646 kb on - strand at 45.647 kb on + strand at 45.647 kb on + strand at 45.647 kb on + strand at 45.717 kb on - strand at 45.717 kb on - strand at 45.773 kb on + strand, within MMJJ_RS00235 at 45.814 kb on + strand, within MMJJ_RS00235 at 45.837 kb on - strand, within MMJJ_RS00235 at 45.837 kb on - strand, within MMJJ_RS00235 at 45.881 kb on - strand, within MMJJ_RS00235 at 45.881 kb on - strand, within MMJJ_RS00235 at 45.881 kb on - strand, within MMJJ_RS00235 at 45.882 kb on + strand, within MMJJ_RS00235 at 45.882 kb on + strand, within MMJJ_RS00235 at 45.885 kb on - strand, within MMJJ_RS00235 at 45.886 kb on + strand, within MMJJ_RS00235 at 45.906 kb on + strand, within MMJJ_RS00235 at 45.926 kb on - strand, within MMJJ_RS00235 at 45.977 kb on + strand, within MMJJ_RS00235 at 45.977 kb on + strand, within MMJJ_RS00235 at 46.002 kb on - strand, within MMJJ_RS00235 at 46.002 kb on - strand, within MMJJ_RS00235 at 46.002 kb on - strand, within MMJJ_RS00235 at 46.003 kb on + strand, within MMJJ_RS00235 at 46.003 kb on + strand, within MMJJ_RS00235 at 46.007 kb on + strand, within MMJJ_RS00235 at 46.007 kb on + strand, within MMJJ_RS00235 at 46.007 kb on + strand, within MMJJ_RS00235 at 46.033 kb on - strand, within MMJJ_RS00235 at 46.034 kb on + strand, within MMJJ_RS00235 at 46.075 kb on - strand, within MMJJ_RS00235 at 46.076 kb on + strand, within MMJJ_RS00235 at 46.106 kb on - strand, within MMJJ_RS00235 at 46.107 kb on + strand, within MMJJ_RS00235 at 46.120 kb on - strand, within MMJJ_RS00235 at 46.135 kb on + strand, within MMJJ_RS00235 at 46.152 kb on + strand, within MMJJ_RS00235 at 46.252 kb on + strand, within MMJJ_RS00235 at 46.336 kb on + strand, within MMJJ_RS00235 at 46.338 kb on - strand, within MMJJ_RS00235 at 46.339 kb on + strand, within MMJJ_RS00235 at 46.339 kb on + strand, within MMJJ_RS00235 at 46.394 kb on - strand, within MMJJ_RS00235 at 46.426 kb on - strand, within MMJJ_RS00235 at 46.430 kb on - strand, within MMJJ_RS00235 at 46.445 kb on - strand, within MMJJ_RS00235 at 46.480 kb on + strand, within MMJJ_RS00235 at 46.480 kb on + strand, within MMJJ_RS00235 at 46.586 kb on + strand, within MMJJ_RS00235 at 46.586 kb on + strand, within MMJJ_RS00235 at 46.594 kb on + strand, within MMJJ_RS00235 at 46.673 kb on - strand, within MMJJ_RS00235 at 46.674 kb on + strand, within MMJJ_RS00235 at 46.681 kb on - strand, within MMJJ_RS00235 at 46.682 kb on + strand, within MMJJ_RS00235 at 46.686 kb on + strand, within MMJJ_RS00235 at 46.686 kb on + strand, within MMJJ_RS00235 at 46.723 kb on - strand, within MMJJ_RS00235 at 46.724 kb on + strand, within MMJJ_RS00235 at 46.730 kb on + strand, within MMJJ_RS00235 at 46.747 kb on + strand, within MMJJ_RS00235 at 46.747 kb on + strand, within MMJJ_RS00235 at 46.760 kb on - strand, within MMJJ_RS00235 at 46.760 kb on - strand, within MMJJ_RS00235 at 46.760 kb on - strand, within MMJJ_RS00235 at 46.761 kb on + strand, within MMJJ_RS00235 at 46.762 kb on - strand, within MMJJ_RS00235 at 46.763 kb on + strand, within MMJJ_RS00235 at 46.763 kb on + strand, within MMJJ_RS00235 at 46.769 kb on - strand, within MMJJ_RS00235 at 46.769 kb on - strand, within MMJJ_RS00235 at 46.774 kb on - strand, within MMJJ_RS00235 at 46.774 kb on - strand, within MMJJ_RS00235 at 46.775 kb on + strand, within MMJJ_RS00235 at 46.847 kb on - strand, within MMJJ_RS00235 at 46.860 kb on - strand, within MMJJ_RS00235 at 47.043 kb on + strand, within MMJJ_RS00235 at 47.060 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.174 kb on - strand, within MMJJ_RS00235 at 47.175 kb on + strand, within MMJJ_RS00235 at 47.175 kb on + strand, within MMJJ_RS00235 at 47.175 kb on + strand, within MMJJ_RS00235 at 47.346 kb on + strand at 47.389 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.423 kb on - strand at 47.424 kb on + strand at 47.424 kb on + strand at 47.424 kb on + strand at 47.424 kb on + strand at 47.424 kb on + strand at 47.424 kb on + strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.425 kb on - strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.426 kb on + strand at 47.427 kb on - strand at 47.427 kb on - strand at 47.427 kb on - strand at 47.427 kb on - strand at 47.427 kb on - strand at 47.427 kb on - strand at 47.428 kb on + strand at 47.428 kb on + strand at 47.428 kb on + strand at 47.450 kb on - strand at 47.451 kb on + strand at 47.451 kb on + strand at 47.454 kb on - strand at 47.460 kb on - strand at 47.471 kb on - strand at 47.472 kb on + strand at 47.472 kb on + strand at 47.477 kb on - strand at 47.478 kb on + strand at 47.478 kb on + strand at 47.479 kb on - strand at 47.479 kb on - strand at 47.494 kb on + strand at 47.494 kb on + strand at 47.529 kb on - strand at 47.530 kb on + strand at 47.553 kb on + strand at 47.555 kb on - strand at 47.555 kb on - strand at 47.568 kb on + strand at 47.576 kb on - strand at 47.576 kb on - strand at 47.577 kb on + strand at 47.577 kb on + strand at 47.577 kb on + strand at 47.597 kb on - strand at 47.598 kb on + strand at 47.598 kb on + strand at 47.598 kb on + strand at 47.602 kb on + strand at 47.629 kb on - strand, within MMJJ_RS00240 at 47.665 kb on - strand, within MMJJ_RS00240 at 47.668 kb on + strand, within MMJJ_RS00240 at 47.690 kb on - strand, within MMJJ_RS00240 at 47.697 kb on - strand, within MMJJ_RS00240 at 47.698 kb on + strand, within MMJJ_RS00240 at 47.709 kb on + strand, within MMJJ_RS00240 at 47.724 kb on + strand, within MMJJ_RS00240 at 47.741 kb on - strand, within MMJJ_RS00240 at 47.779 kb on - strand, within MMJJ_RS00240 at 47.779 kb on - strand, within MMJJ_RS00240 at 47.797 kb on - strand, within MMJJ_RS00240 at 47.798 kb on + strand, within MMJJ_RS00240 at 47.799 kb on - strand, within MMJJ_RS00240 at 47.799 kb on - strand, within MMJJ_RS00240 at 47.799 kb on - strand, within MMJJ_RS00240 at 47.799 kb on - strand, within MMJJ_RS00240 at 47.799 kb on - strand, within MMJJ_RS00240 at 47.799 kb on - strand, within MMJJ_RS00240 at 47.800 kb on + strand, within MMJJ_RS00240 at 47.800 kb on + strand, within MMJJ_RS00240 at 47.800 kb on + strand, within MMJJ_RS00240 at 47.804 kb on - strand, within MMJJ_RS00240 at 47.811 kb on - strand, within MMJJ_RS00240 at 47.814 kb on + strand, within MMJJ_RS00240 at 47.818 kb on - strand, within MMJJ_RS00240 at 47.819 kb on + strand, within MMJJ_RS00240 at 47.819 kb on + strand, within MMJJ_RS00240 at 47.819 kb on + strand, within MMJJ_RS00240 at 47.820 kb on - strand, within MMJJ_RS00240 at 47.822 kb on - strand, within MMJJ_RS00240 at 47.822 kb on - strand, within MMJJ_RS00240 at 47.823 kb on + strand, within MMJJ_RS00240 at 47.828 kb on - strand, within MMJJ_RS00240 at 47.828 kb on - strand, within MMJJ_RS00240 at 47.829 kb on + strand, within MMJJ_RS00240 at 47.853 kb on + strand, within MMJJ_RS00240 at 47.921 kb on + strand, within MMJJ_RS00240 at 47.934 kb on + strand, within MMJJ_RS00240 at 47.956 kb on + strand, within MMJJ_RS00240 at 47.971 kb on - strand, within MMJJ_RS00240 at 47.971 kb on - strand, within MMJJ_RS00240 at 47.974 kb on - strand, within MMJJ_RS00240 at 47.994 kb on - strand, within MMJJ_RS00240 at 47.995 kb on + strand, within MMJJ_RS00240 at 47.995 kb on + strand, within MMJJ_RS00240 at 47.995 kb on + strand, within MMJJ_RS00240 at 47.995 kb on + strand, within MMJJ_RS00240 at 48.080 kb on + strand, within MMJJ_RS00240 at 48.080 kb on + strand, within MMJJ_RS00240 at 48.118 kb on + strand, within MMJJ_RS00240 at 48.233 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.235 kb on - strand, within MMJJ_RS00240 at 48.236 kb on + strand, within MMJJ_RS00240 at 48.236 kb on + strand, within MMJJ_RS00240 at 48.236 kb on + strand, within MMJJ_RS00240 at 48.236 kb on + strand, within MMJJ_RS00240 at 48.236 kb on + strand, within MMJJ_RS00240 at 48.257 kb on + strand, within MMJJ_RS00240 at 48.290 kb on - strand, within MMJJ_RS00240 at 48.338 kb on - strand, within MMJJ_RS00240 at 48.338 kb on - strand, within MMJJ_RS00240 at 48.356 kb on - strand, within MMJJ_RS00240 at 48.357 kb on + strand, within MMJJ_RS00240 at 48.372 kb on - strand, within MMJJ_RS00240
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 45,483 - -3.2 45,506 - +1.6 45,532 + +0.2 45,564 + +0.0 45,609 - -2.3 45,642 - -3.8 45,646 - -3.3 45,646 - -4.8 45,646 - -2.3 45,647 + -1.7 45,647 + -4.4 45,647 + -2.9 45,717 - -3.4 45,717 - -1.6 45,773 + MMJJ_RS00235 0.10 -3.3 45,814 + MMJJ_RS00235 0.13 -3.9 45,837 - MMJJ_RS00235 0.14 -2.6 45,837 - MMJJ_RS00235 0.14 -3.2 45,881 - MMJJ_RS00235 0.16 -3.8 45,881 - MMJJ_RS00235 0.16 -4.5 45,881 - MMJJ_RS00235 0.16 -2.1 45,882 + MMJJ_RS00235 0.16 -1.9 45,882 + MMJJ_RS00235 0.16 -4.8 45,885 - MMJJ_RS00235 0.16 -4.7 45,886 + MMJJ_RS00235 0.17 -4.5 45,906 + MMJJ_RS00235 0.18 -3.6 45,926 - MMJJ_RS00235 0.19 -4.0 45,977 + MMJJ_RS00235 0.21 -2.7 45,977 + MMJJ_RS00235 0.21 -4.1 46,002 - MMJJ_RS00235 0.23 -4.8 46,002 - MMJJ_RS00235 0.23 -2.6 46,002 - MMJJ_RS00235 0.23 -1.2 46,003 + MMJJ_RS00235 0.23 -4.5 46,003 + MMJJ_RS00235 0.23 -3.9 46,007 + MMJJ_RS00235 0.23 -3.5 46,007 + MMJJ_RS00235 0.23 -3.8 46,007 + MMJJ_RS00235 0.23 -4.6 46,033 - MMJJ_RS00235 0.24 -3.0 46,034 + MMJJ_RS00235 0.25 -4.9 46,075 - MMJJ_RS00235 0.27 -2.9 46,076 + MMJJ_RS00235 0.27 -4.6 46,106 - MMJJ_RS00235 0.28 -3.1 46,107 + MMJJ_RS00235 0.29 -2.6 46,120 - MMJJ_RS00235 0.29 -2.2 46,135 + MMJJ_RS00235 0.30 -0.4 46,152 + MMJJ_RS00235 0.31 -3.7 46,252 + MMJJ_RS00235 0.36 -3.6 46,336 + MMJJ_RS00235 0.41 -2.0 46,338 - MMJJ_RS00235 0.41 -4.2 46,339 + MMJJ_RS00235 0.41 -3.9 46,339 + MMJJ_RS00235 0.41 -3.2 46,394 - MMJJ_RS00235 0.44 -2.2 46,426 - MMJJ_RS00235 0.46 -0.3 46,430 - MMJJ_RS00235 0.46 -4.0 46,445 - MMJJ_RS00235 0.47 -1.7 46,480 + MMJJ_RS00235 0.49 -4.4 46,480 + MMJJ_RS00235 0.49 -2.1 46,586 + MMJJ_RS00235 0.55 -3.8 46,586 + MMJJ_RS00235 0.55 -4.0 46,594 + MMJJ_RS00235 0.55 -2.9 46,673 - MMJJ_RS00235 0.59 -3.1 46,674 + MMJJ_RS00235 0.59 -0.4 46,681 - MMJJ_RS00235 0.60 -5.1 46,682 + MMJJ_RS00235 0.60 -4.2 46,686 + MMJJ_RS00235 0.60 -4.4 46,686 + MMJJ_RS00235 0.60 -2.5 46,723 - MMJJ_RS00235 0.62 -4.6 46,724 + MMJJ_RS00235 0.62 -4.3 46,730 + MMJJ_RS00235 0.62 -3.5 46,747 + MMJJ_RS00235 0.63 -2.0 46,747 + MMJJ_RS00235 0.63 -3.0 46,760 - MMJJ_RS00235 0.64 -3.0 46,760 - MMJJ_RS00235 0.64 -4.9 46,760 - MMJJ_RS00235 0.64 -4.0 46,761 + MMJJ_RS00235 0.64 -3.3 46,762 - MMJJ_RS00235 0.64 -2.8 46,763 + MMJJ_RS00235 0.64 -4.5 46,763 + MMJJ_RS00235 0.64 -4.0 46,769 - MMJJ_RS00235 0.64 -3.3 46,769 - MMJJ_RS00235 0.64 -5.6 46,774 - MMJJ_RS00235 0.65 -4.8 46,774 - MMJJ_RS00235 0.65 -4.9 46,775 + MMJJ_RS00235 0.65 -3.3 46,847 - MMJJ_RS00235 0.69 -3.6 46,860 - MMJJ_RS00235 0.69 -1.7 47,043 + MMJJ_RS00235 0.79 -4.0 47,060 - MMJJ_RS00235 0.80 -1.5 47,174 - MMJJ_RS00235 0.86 -2.9 47,174 - MMJJ_RS00235 0.86 -2.7 47,174 - MMJJ_RS00235 0.86 -3.9 47,174 - MMJJ_RS00235 0.86 -2.0 47,174 - MMJJ_RS00235 0.86 -3.0 47,174 - MMJJ_RS00235 0.86 -3.7 47,174 - MMJJ_RS00235 0.86 -5.7 47,175 + MMJJ_RS00235 0.86 -2.9 47,175 + MMJJ_RS00235 0.86 -1.5 47,175 + MMJJ_RS00235 0.86 -4.6 47,346 + -2.7 47,389 - -2.9 47,423 - -4.8 47,423 - -4.5 47,423 - -3.0 47,423 - -1.9 47,423 - -4.2 47,423 - -2.9 47,423 - -2.5 47,423 - -2.6 47,423 - -4.1 47,424 + -1.5 47,424 + -3.8 47,424 + -2.8 47,424 + -2.6 47,424 + -3.5 47,424 + -1.7 47,425 - -3.6 47,425 - -3.7 47,425 - -3.7 47,425 - -4.6 47,425 - -1.1 47,425 - -3.3 47,425 - -4.7 47,425 - -3.9 47,425 - -2.5 47,426 + -4.6 47,426 + -2.5 47,426 + -3.0 47,426 + -5.4 47,426 + -1.6 47,426 + -5.2 47,426 + -4.2 47,426 + -3.3 47,427 - -3.2 47,427 - -2.9 47,427 - -3.7 47,427 - -3.7 47,427 - -4.0 47,427 - -3.3 47,428 + -4.0 47,428 + -5.1 47,428 + -3.4 47,450 - +0.3 47,451 + +0.4 47,451 + -1.3 47,454 - +0.1 47,460 - -1.4 47,471 - -0.8 47,472 + -0.5 47,472 + +0.0 47,477 - -0.7 47,478 + -0.4 47,478 + -0.4 47,479 - +0.2 47,479 - -1.4 47,494 + -0.6 47,494 + +1.2 47,529 - +0.1 47,530 + -1.2 47,553 + +1.1 47,555 - -0.1 47,555 - -0.5 47,568 + +0.5 47,576 - -0.4 47,576 - +0.1 47,577 + +0.8 47,577 + +0.6 47,577 + -0.2 47,597 - +0.4 47,598 + -1.0 47,598 + +1.4 47,598 + +0.6 47,602 + +0.1 47,629 - MMJJ_RS00240 0.12 -0.4 47,665 - MMJJ_RS00240 0.15 +0.1 47,668 + MMJJ_RS00240 0.15 +0.8 47,690 - MMJJ_RS00240 0.18 +0.1 47,697 - MMJJ_RS00240 0.18 -0.9 47,698 + MMJJ_RS00240 0.18 +0.3 47,709 + MMJJ_RS00240 0.19 +0.8 47,724 + MMJJ_RS00240 0.21 +0.2 47,741 - MMJJ_RS00240 0.23 +1.4 47,779 - MMJJ_RS00240 0.26 +0.1 47,779 - MMJJ_RS00240 0.26 +0.0 47,797 - MMJJ_RS00240 0.28 -0.1 47,798 + MMJJ_RS00240 0.28 -0.6 47,799 - MMJJ_RS00240 0.28 +0.1 47,799 - MMJJ_RS00240 0.28 -0.1 47,799 - MMJJ_RS00240 0.28 +0.5 47,799 - MMJJ_RS00240 0.28 +0.7 47,799 - MMJJ_RS00240 0.28 +0.2 47,799 - MMJJ_RS00240 0.28 +0.4 47,800 + MMJJ_RS00240 0.28 -0.9 47,800 + MMJJ_RS00240 0.28 +0.7 47,800 + MMJJ_RS00240 0.28 +0.6 47,804 - MMJJ_RS00240 0.29 +0.7 47,811 - MMJJ_RS00240 0.30 -0.6 47,814 + MMJJ_RS00240 0.30 -0.8 47,818 - MMJJ_RS00240 0.30 -0.0 47,819 + MMJJ_RS00240 0.30 +0.9 47,819 + MMJJ_RS00240 0.30 +0.8 47,819 + MMJJ_RS00240 0.30 -3.4 47,820 - MMJJ_RS00240 0.30 -0.6 47,822 - MMJJ_RS00240 0.31 -0.1 47,822 - MMJJ_RS00240 0.31 +0.3 47,823 + MMJJ_RS00240 0.31 -0.9 47,828 - MMJJ_RS00240 0.31 -2.9 47,828 - MMJJ_RS00240 0.31 -0.3 47,829 + MMJJ_RS00240 0.31 +1.0 47,853 + MMJJ_RS00240 0.34 -0.6 47,921 + MMJJ_RS00240 0.40 +0.7 47,934 + MMJJ_RS00240 0.42 +0.5 47,956 + MMJJ_RS00240 0.44 +0.0 47,971 - MMJJ_RS00240 0.45 +0.7 47,971 - MMJJ_RS00240 0.45 +0.6 47,974 - MMJJ_RS00240 0.46 +0.5 47,994 - MMJJ_RS00240 0.48 -1.2 47,995 + MMJJ_RS00240 0.48 -0.0 47,995 + MMJJ_RS00240 0.48 +3.4 47,995 + MMJJ_RS00240 0.48 -0.7 47,995 + MMJJ_RS00240 0.48 +0.4 48,080 + MMJJ_RS00240 0.56 -0.5 48,080 + MMJJ_RS00240 0.56 -1.4 48,118 + MMJJ_RS00240 0.60 -0.9 48,233 - MMJJ_RS00240 0.71 +0.2 48,235 - MMJJ_RS00240 0.72 +1.0 48,235 - MMJJ_RS00240 0.72 +0.0 48,235 - MMJJ_RS00240 0.72 -0.6 48,235 - MMJJ_RS00240 0.72 +0.7 48,235 - MMJJ_RS00240 0.72 -0.5 48,235 - MMJJ_RS00240 0.72 +1.0 48,235 - MMJJ_RS00240 0.72 -0.5 48,235 - MMJJ_RS00240 0.72 +0.5 48,236 + MMJJ_RS00240 0.72 +0.9 48,236 + MMJJ_RS00240 0.72 -0.3 48,236 + MMJJ_RS00240 0.72 -0.3 48,236 + MMJJ_RS00240 0.72 -0.3 48,236 + MMJJ_RS00240 0.72 +0.1 48,257 + MMJJ_RS00240 0.74 -0.5 48,290 - MMJJ_RS00240 0.77 -1.0 48,338 - MMJJ_RS00240 0.82 +0.5 48,338 - MMJJ_RS00240 0.82 +0.3 48,356 - MMJJ_RS00240 0.83 +1.0 48,357 + MMJJ_RS00240 0.84 -2.3 48,372 - MMJJ_RS00240 0.85 +0.7
Or see this region's nucleotide sequence