Experiment: D-Trehalose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01455 and CA265_RS01460 are separated by 54 nucleotides CA265_RS01460 and CA265_RS01465 are separated by 338 nucleotides CA265_RS01465 and CA265_RS01470 are separated by 262 nucleotides CA265_RS01470 and CA265_RS01475 are separated by 172 nucleotides
CA265_RS01455: CA265_RS01455 - MFS transporter, at 371,177 to 372,418
_RS01455
CA265_RS01460: CA265_RS01460 - hypothetical protein, at 372,473 to 372,841
_RS01460
CA265_RS01465: CA265_RS01465 - hypothetical protein, at 373,180 to 373,386
_RS01465
CA265_RS01470: CA265_RS01470 - tRNA-Ser, at 373,649 to 373,736
_RS01470
CA265_RS01475: CA265_RS01475 - DoxX family protein, at 373,909 to 374,355
_RS01475
Position (kb) 373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 372.241 kb on + strand, within CA265_RS01455 at 372.246 kb on + strand, within CA265_RS01455 at 372.264 kb on + strand, within CA265_RS01455 at 372.278 kb on + strand, within CA265_RS01455 at 372.300 kb on + strand at 372.322 kb on + strand at 372.323 kb on - strand at 372.427 kb on - strand at 372.552 kb on + strand, within CA265_RS01460 at 372.568 kb on + strand, within CA265_RS01460 at 372.609 kb on + strand, within CA265_RS01460 at 372.651 kb on - strand, within CA265_RS01460 at 372.679 kb on + strand, within CA265_RS01460 at 372.734 kb on + strand, within CA265_RS01460 at 372.735 kb on - strand, within CA265_RS01460 at 372.735 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.779 kb on - strand, within CA265_RS01460 at 372.881 kb on - strand at 372.916 kb on - strand at 372.941 kb on + strand at 372.942 kb on - strand at 372.943 kb on + strand at 372.965 kb on + strand at 372.966 kb on - strand at 372.969 kb on - strand at 372.985 kb on - strand at 373.026 kb on - strand at 373.027 kb on + strand at 373.127 kb on - strand at 373.130 kb on + strand at 373.206 kb on + strand, within CA265_RS01465 at 373.224 kb on + strand, within CA265_RS01465 at 373.225 kb on - strand, within CA265_RS01465 at 373.225 kb on - strand, within CA265_RS01465 at 373.244 kb on + strand, within CA265_RS01465 at 373.246 kb on + strand, within CA265_RS01465 at 373.248 kb on + strand, within CA265_RS01465 at 373.248 kb on + strand, within CA265_RS01465 at 373.322 kb on - strand, within CA265_RS01465 at 373.322 kb on - strand, within CA265_RS01465 at 373.336 kb on - strand, within CA265_RS01465 at 373.420 kb on + strand at 373.421 kb on - strand at 373.440 kb on + strand at 373.445 kb on + strand at 373.445 kb on + strand at 373.446 kb on - strand at 373.530 kb on + strand at 373.531 kb on - strand at 373.531 kb on - strand at 373.588 kb on - strand at 373.588 kb on - strand at 373.597 kb on + strand at 373.623 kb on - strand at 373.623 kb on - strand at 373.623 kb on - strand at 373.623 kb on - strand at 373.805 kb on - strand at 373.868 kb on + strand at 373.946 kb on - strand at 373.993 kb on + strand, within CA265_RS01475 at 373.997 kb on - strand, within CA265_RS01475 at 374.050 kb on - strand, within CA265_RS01475 at 374.089 kb on - strand, within CA265_RS01475 at 374.101 kb on + strand, within CA265_RS01475 at 374.124 kb on + strand, within CA265_RS01475 at 374.125 kb on - strand, within CA265_RS01475 at 374.131 kb on + strand, within CA265_RS01475 at 374.145 kb on + strand, within CA265_RS01475 at 374.145 kb on + strand, within CA265_RS01475 at 374.146 kb on - strand, within CA265_RS01475 at 374.149 kb on + strand, within CA265_RS01475 at 374.150 kb on - strand, within CA265_RS01475 at 374.150 kb on - strand, within CA265_RS01475 at 374.157 kb on + strand, within CA265_RS01475 at 374.179 kb on + strand, within CA265_RS01475 at 374.224 kb on - strand, within CA265_RS01475 at 374.272 kb on - strand, within CA265_RS01475 at 374.279 kb on - strand, within CA265_RS01475 at 374.281 kb on + strand, within CA265_RS01475 at 374.281 kb on + strand, within CA265_RS01475 at 374.282 kb on - strand, within CA265_RS01475 at 374.289 kb on + strand, within CA265_RS01475
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose 20mM (C) remove 372,241 + CA265_RS01455 0.86 +1.2 372,246 + CA265_RS01455 0.86 -0.6 372,264 + CA265_RS01455 0.88 -0.7 372,278 + CA265_RS01455 0.89 -0.4 372,300 + -0.2 372,322 + +2.0 372,323 - -0.6 372,427 - +2.2 372,552 + CA265_RS01460 0.21 -0.3 372,568 + CA265_RS01460 0.26 -0.2 372,609 + CA265_RS01460 0.37 +0.0 372,651 - CA265_RS01460 0.48 +1.9 372,679 + CA265_RS01460 0.56 -2.0 372,734 + CA265_RS01460 0.71 +1.9 372,735 - CA265_RS01460 0.71 -0.6 372,735 - CA265_RS01460 0.71 +1.6 372,742 - CA265_RS01460 0.73 +1.6 372,742 - CA265_RS01460 0.73 +0.8 372,742 - CA265_RS01460 0.73 +0.3 372,742 - CA265_RS01460 0.73 +0.9 372,779 - CA265_RS01460 0.83 -0.3 372,881 - +0.1 372,916 - +1.3 372,941 + +0.3 372,942 - -0.4 372,943 + +0.2 372,965 + -0.2 372,966 - -1.3 372,969 - -0.3 372,985 - +0.1 373,026 - -1.1 373,027 + -0.4 373,127 - +1.5 373,130 + -2.8 373,206 + CA265_RS01465 0.13 -0.1 373,224 + CA265_RS01465 0.21 +0.4 373,225 - CA265_RS01465 0.22 -0.1 373,225 - CA265_RS01465 0.22 -0.4 373,244 + CA265_RS01465 0.31 -0.5 373,246 + CA265_RS01465 0.32 +1.1 373,248 + CA265_RS01465 0.33 -0.2 373,248 + CA265_RS01465 0.33 +0.0 373,322 - CA265_RS01465 0.69 -1.3 373,322 - CA265_RS01465 0.69 -0.6 373,336 - CA265_RS01465 0.75 +0.0 373,420 + -2.0 373,421 - +0.7 373,440 + +1.4 373,445 + +1.4 373,445 + -0.4 373,446 - -0.0 373,530 + +1.6 373,531 - +0.2 373,531 - -1.2 373,588 - -0.1 373,588 - +1.2 373,597 + +0.4 373,623 - -3.2 373,623 - +1.1 373,623 - -0.2 373,623 - -0.3 373,805 - -2.0 373,868 + +0.9 373,946 - +2.6 373,993 + CA265_RS01475 0.19 +0.4 373,997 - CA265_RS01475 0.20 -1.6 374,050 - CA265_RS01475 0.32 +0.8 374,089 - CA265_RS01475 0.40 -0.4 374,101 + CA265_RS01475 0.43 +1.0 374,124 + CA265_RS01475 0.48 -1.7 374,125 - CA265_RS01475 0.48 -0.4 374,131 + CA265_RS01475 0.50 -1.4 374,145 + CA265_RS01475 0.53 -0.7 374,145 + CA265_RS01475 0.53 +0.3 374,146 - CA265_RS01475 0.53 +0.2 374,149 + CA265_RS01475 0.54 +0.0 374,150 - CA265_RS01475 0.54 -0.2 374,150 - CA265_RS01475 0.54 -0.2 374,157 + CA265_RS01475 0.55 -0.9 374,179 + CA265_RS01475 0.60 +1.0 374,224 - CA265_RS01475 0.70 -0.1 374,272 - CA265_RS01475 0.81 -0.7 374,279 - CA265_RS01475 0.83 -1.3 374,281 + CA265_RS01475 0.83 +0.4 374,281 + CA265_RS01475 0.83 -0.0 374,282 - CA265_RS01475 0.83 +0.1 374,289 + CA265_RS01475 0.85 -0.7
Or see this region's nucleotide sequence