Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02810 and AO356_02815 are separated by 25 nucleotides AO356_02815 and AO356_02820 are separated by 126 nucleotides AO356_02820 and AO356_02825 are separated by 157 nucleotides
AO356_02810: AO356_02810 - CmaX protein, at 618,308 to 619,303
_02810
AO356_02815: AO356_02815 - ribonuclease activity regulator protein RraA, at 619,329 to 619,820
_02815
AO356_02820: AO356_02820 - EstX protein, at 619,947 to 620,936
_02820
AO356_02825: AO356_02825 - phosphoenolpyruvate synthase, at 621,094 to 623,469
_02825
Position (kb)
619
620
621 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 619.385 kb on - strand, within AO356_02815 at 619.390 kb on + strand, within AO356_02815 at 619.390 kb on + strand, within AO356_02815 at 619.390 kb on + strand, within AO356_02815 at 619.390 kb on + strand, within AO356_02815 at 619.390 kb on + strand, within AO356_02815 at 619.391 kb on - strand, within AO356_02815 at 619.391 kb on - strand, within AO356_02815 at 619.391 kb on - strand, within AO356_02815 at 619.391 kb on - strand, within AO356_02815 at 619.391 kb on - strand, within AO356_02815 at 619.391 kb on - strand, within AO356_02815 at 619.540 kb on + strand, within AO356_02815 at 619.540 kb on + strand, within AO356_02815 at 619.540 kb on + strand, within AO356_02815 at 619.541 kb on - strand, within AO356_02815 at 619.810 kb on + strand at 619.810 kb on + strand at 619.810 kb on + strand at 619.811 kb on - strand at 619.811 kb on - strand at 619.811 kb on - strand at 619.811 kb on - strand at 619.895 kb on + strand at 619.901 kb on - strand at 620.285 kb on + strand, within AO356_02820 at 620.285 kb on + strand, within AO356_02820 at 620.286 kb on - strand, within AO356_02820 at 620.286 kb on - strand, within AO356_02820 at 620.286 kb on - strand, within AO356_02820 at 620.413 kb on + strand, within AO356_02820 at 620.413 kb on + strand, within AO356_02820 at 620.414 kb on - strand, within AO356_02820 at 620.414 kb on - strand, within AO356_02820 at 620.474 kb on - strand, within AO356_02820 at 620.474 kb on - strand, within AO356_02820 at 620.474 kb on - strand, within AO356_02820 at 620.594 kb on - strand, within AO356_02820 at 620.594 kb on - strand, within AO356_02820 at 620.693 kb on - strand, within AO356_02820 at 620.722 kb on + strand, within AO356_02820 at 620.723 kb on - strand, within AO356_02820 at 620.723 kb on - strand, within AO356_02820 at 620.830 kb on + strand, within AO356_02820 at 620.860 kb on + strand at 620.861 kb on - strand at 620.937 kb on - strand at 620.937 kb on - strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.051 kb on + strand at 621.065 kb on + strand at 621.065 kb on + strand at 621.066 kb on - strand at 621.066 kb on - strand at 621.066 kb on - strand at 621.239 kb on + strand at 621.639 kb on + strand, within AO356_02825 at 621.639 kb on + strand, within AO356_02825 at 621.639 kb on + strand, within AO356_02825 at 621.639 kb on + strand, within AO356_02825 at 621.641 kb on + strand, within AO356_02825 at 621.642 kb on - strand, within AO356_02825 at 621.692 kb on + strand, within AO356_02825 at 621.693 kb on - strand, within AO356_02825 at 621.719 kb on + strand, within AO356_02825 at 621.815 kb on + strand, within AO356_02825 at 621.836 kb on + strand, within AO356_02825 at 621.890 kb on + strand, within AO356_02825 at 621.890 kb on + strand, within AO356_02825 at 621.891 kb on - strand, within AO356_02825 at 621.891 kb on - strand, within AO356_02825
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 619,385 - AO356_02815 0.11 +0.3 619,390 + AO356_02815 0.12 +0.4 619,390 + AO356_02815 0.12 -1.7 619,390 + AO356_02815 0.12 -0.8 619,390 + AO356_02815 0.12 -0.0 619,390 + AO356_02815 0.12 -2.3 619,391 - AO356_02815 0.13 +1.9 619,391 - AO356_02815 0.13 -0.4 619,391 - AO356_02815 0.13 +0.0 619,391 - AO356_02815 0.13 -0.6 619,391 - AO356_02815 0.13 +1.5 619,391 - AO356_02815 0.13 +0.2 619,540 + AO356_02815 0.43 +0.4 619,540 + AO356_02815 0.43 -1.1 619,540 + AO356_02815 0.43 -0.4 619,541 - AO356_02815 0.43 -0.3 619,810 + -2.6 619,810 + -0.3 619,810 + -0.5 619,811 - -1.1 619,811 - -0.1 619,811 - -0.7 619,811 - -0.8 619,895 + -2.9 619,901 - +1.0 620,285 + AO356_02820 0.34 +0.3 620,285 + AO356_02820 0.34 -0.8 620,286 - AO356_02820 0.34 +0.9 620,286 - AO356_02820 0.34 +0.3 620,286 - AO356_02820 0.34 -0.9 620,413 + AO356_02820 0.47 -1.1 620,413 + AO356_02820 0.47 -0.1 620,414 - AO356_02820 0.47 -1.0 620,414 - AO356_02820 0.47 -0.3 620,474 - AO356_02820 0.53 +0.6 620,474 - AO356_02820 0.53 -0.1 620,474 - AO356_02820 0.53 +1.4 620,594 - AO356_02820 0.65 -0.3 620,594 - AO356_02820 0.65 +0.1 620,693 - AO356_02820 0.75 -0.6 620,722 + AO356_02820 0.78 +0.3 620,723 - AO356_02820 0.78 -1.3 620,723 - AO356_02820 0.78 +1.0 620,830 + AO356_02820 0.89 +0.0 620,860 + -0.6 620,861 - +0.9 620,937 - -0.2 620,937 - +1.0 621,051 + +0.2 621,051 + +0.0 621,051 + +0.3 621,051 + -1.4 621,051 + +1.4 621,051 + -0.4 621,051 + -1.5 621,051 + +0.5 621,051 + -0.6 621,065 + -0.8 621,065 + -0.6 621,066 - +0.4 621,066 - -0.4 621,066 - -0.4 621,239 + -0.1 621,639 + AO356_02825 0.23 +0.2 621,639 + AO356_02825 0.23 -0.5 621,639 + AO356_02825 0.23 -0.5 621,639 + AO356_02825 0.23 +0.5 621,641 + AO356_02825 0.23 +0.0 621,642 - AO356_02825 0.23 +1.1 621,692 + AO356_02825 0.25 -0.3 621,693 - AO356_02825 0.25 -0.3 621,719 + AO356_02825 0.26 +0.4 621,815 + AO356_02825 0.30 +0.1 621,836 + AO356_02825 0.31 -1.8 621,890 + AO356_02825 0.34 -1.0 621,890 + AO356_02825 0.34 +0.4 621,891 - AO356_02825 0.34 -0.6 621,891 - AO356_02825 0.34 -1.5
Or see this region's nucleotide sequence