Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3209 and BT3210 are separated by 3 nucleotides BT3210 and BT3211 are separated by 49 nucleotides
BT3209: BT3209 - conserved hypothetical protein (NCBI ptt file), at 4,102,964 to 4,103,803
BT3209
BT3210: BT3210 - cell division protein FtsX (NCBI ptt file), at 4,103,807 to 4,104,688
BT3210
BT3211: BT3211 - conserved hypothetical protein (NCBI ptt file), at 4,104,738 to 4,104,971
BT3211
Position (kb)
4102
4103
4104 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4101.992 kb on + strand at 4101.992 kb on + strand at 4101.993 kb on - strand at 4102.007 kb on + strand at 4102.007 kb on + strand at 4102.016 kb on - strand at 4102.035 kb on + strand at 4102.040 kb on + strand at 4102.081 kb on + strand at 4102.117 kb on + strand at 4102.118 kb on - strand at 4102.118 kb on - strand at 4102.118 kb on - strand at 4102.118 kb on - strand at 4102.141 kb on - strand at 4102.147 kb on + strand at 4102.148 kb on - strand at 4102.198 kb on - strand at 4102.225 kb on - strand at 4102.248 kb on + strand at 4102.265 kb on - strand at 4102.292 kb on + strand at 4102.293 kb on - strand at 4102.296 kb on + strand at 4102.319 kb on - strand at 4102.409 kb on + strand at 4102.512 kb on - strand at 4102.752 kb on + strand at 4102.753 kb on - strand at 4102.764 kb on + strand at 4102.782 kb on + strand at 4102.920 kb on + strand at 4103.138 kb on + strand, within BT3209 at 4103.138 kb on + strand, within BT3209 at 4103.148 kb on + strand, within BT3209 at 4103.156 kb on + strand, within BT3209 at 4103.156 kb on + strand, within BT3209 at 4103.158 kb on + strand, within BT3209 at 4103.510 kb on + strand, within BT3209 at 4103.657 kb on + strand, within BT3209 at 4103.657 kb on + strand, within BT3209 at 4103.657 kb on + strand, within BT3209 at 4103.661 kb on + strand, within BT3209 at 4103.717 kb on + strand, within BT3209 at 4103.825 kb on + strand at 4103.827 kb on + strand at 4103.827 kb on + strand at 4103.848 kb on + strand at 4103.869 kb on + strand at 4103.935 kb on + strand, within BT3210 at 4103.943 kb on + strand, within BT3210 at 4104.004 kb on + strand, within BT3210 at 4104.234 kb on + strand, within BT3210 at 4104.285 kb on + strand, within BT3210 at 4104.334 kb on + strand, within BT3210
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 4,101,992 + -0.8 4,101,992 + -2.2 4,101,993 - -0.0 4,102,007 + -0.5 4,102,007 + -2.8 4,102,016 - -2.9 4,102,035 + -0.3 4,102,040 + +0.9 4,102,081 + +2.6 4,102,117 + +0.2 4,102,118 - -1.1 4,102,118 - -1.4 4,102,118 - +0.3 4,102,118 - -1.9 4,102,141 - +0.5 4,102,147 + -1.8 4,102,148 - +1.6 4,102,198 - +1.1 4,102,225 - -0.8 4,102,248 + -0.9 4,102,265 - +1.1 4,102,292 + -2.1 4,102,293 - -4.8 4,102,296 + -1.6 4,102,319 - +1.1 4,102,409 + +0.6 4,102,512 - +0.2 4,102,752 + +1.6 4,102,753 - -2.2 4,102,764 + -0.1 4,102,782 + -0.7 4,102,920 + -1.5 4,103,138 + BT3209 0.21 -1.6 4,103,138 + BT3209 0.21 -2.8 4,103,148 + BT3209 0.22 -2.9 4,103,156 + BT3209 0.23 -2.6 4,103,156 + BT3209 0.23 -1.3 4,103,158 + BT3209 0.23 -1.3 4,103,510 + BT3209 0.65 +0.6 4,103,657 + BT3209 0.82 -2.2 4,103,657 + BT3209 0.82 -2.1 4,103,657 + BT3209 0.82 -2.5 4,103,661 + BT3209 0.83 -1.1 4,103,717 + BT3209 0.90 -2.8 4,103,825 + +0.9 4,103,827 + -3.1 4,103,827 + -1.8 4,103,848 + -1.9 4,103,869 + -0.4 4,103,935 + BT3210 0.15 -1.0 4,103,943 + BT3210 0.15 +0.4 4,104,004 + BT3210 0.22 -1.8 4,104,234 + BT3210 0.48 -2.0 4,104,285 + BT3210 0.54 -0.6 4,104,334 + BT3210 0.60 -1.6
Or see this region's nucleotide sequence