Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3141 and BT3142 are separated by 184 nucleotides BT3142 and BT3143 overlap by 4 nucleotides BT3143 and BT3144 are separated by 47 nucleotides
BT3141: BT3141 - hypothetical protein (NCBI ptt file), at 4,006,923 to 4,007,405
BT3141
BT3142: BT3142 - mobilization protein BmgB (NCBI ptt file), at 4,007,590 to 4,008,003
BT3142
BT3143: BT3143 - mobilization protein BmgA (NCBI ptt file), at 4,008,000 to 4,008,950
BT3143
BT3144: BT3144 - conserved hypothetical protein (NCBI ptt file), at 4,008,998 to 4,009,738
BT3144
Position (kb)
4007
4008
4009 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4007.007 kb on - strand, within BT3141 at 4007.007 kb on - strand, within BT3141 at 4007.034 kb on - strand, within BT3141 at 4007.040 kb on - strand, within BT3141 at 4007.040 kb on - strand at 4007.059 kb on + strand, within BT3141 at 4007.114 kb on + strand, within BT3141 at 4007.197 kb on + strand, within BT3141 at 4007.449 kb on + strand at 4007.486 kb on - strand at 4007.567 kb on - strand at 4007.585 kb on - strand at 4007.601 kb on - strand at 4007.601 kb on - strand at 4007.696 kb on - strand at 4007.747 kb on - strand, within BT3142 at 4007.749 kb on - strand at 4007.749 kb on - strand, within BT3142 at 4007.894 kb on + strand, within BT3142 at 4007.895 kb on - strand, within BT3142 at 4007.895 kb on - strand, within BT3142 at 4007.895 kb on - strand, within BT3142 at 4007.896 kb on + strand, within BT3142 at 4007.896 kb on + strand, within BT3142 at 4007.897 kb on - strand, within BT3142 at 4007.897 kb on - strand, within BT3142 at 4007.995 kb on - strand at 4008.005 kb on - strand at 4008.005 kb on - strand at 4008.013 kb on + strand at 4008.049 kb on - strand at 4008.149 kb on - strand, within BT3143 at 4008.149 kb on - strand, within BT3143 at 4008.237 kb on - strand, within BT3143 at 4008.237 kb on - strand, within BT3143 at 4008.242 kb on - strand, within BT3143 at 4008.252 kb on + strand, within BT3143 at 4008.253 kb on - strand, within BT3143 at 4008.253 kb on - strand, within BT3143 at 4008.276 kb on - strand, within BT3143 at 4008.337 kb on - strand, within BT3143 at 4008.337 kb on - strand, within BT3143 at 4008.427 kb on - strand, within BT3143 at 4008.439 kb on - strand, within BT3143 at 4008.459 kb on - strand, within BT3143 at 4008.462 kb on - strand at 4008.476 kb on - strand, within BT3143 at 4008.519 kb on - strand, within BT3143 at 4008.657 kb on - strand at 4008.657 kb on - strand, within BT3143 at 4008.737 kb on - strand, within BT3143 at 4008.941 kb on + strand at 4008.985 kb on + strand at 4009.105 kb on + strand, within BT3144 at 4009.105 kb on + strand, within BT3144 at 4009.106 kb on - strand, within BT3144 at 4009.106 kb on - strand, within BT3144 at 4009.149 kb on + strand, within BT3144 at 4009.185 kb on + strand, within BT3144 at 4009.195 kb on + strand, within BT3144 at 4009.196 kb on - strand, within BT3144 at 4009.274 kb on - strand, within BT3144 at 4009.339 kb on + strand, within BT3144 at 4009.342 kb on + strand, within BT3144 at 4009.359 kb on + strand, within BT3144 at 4009.499 kb on + strand, within BT3144 at 4009.499 kb on + strand at 4009.499 kb on + strand, within BT3144 at 4009.579 kb on + strand, within BT3144 at 4009.588 kb on + strand, within BT3144 at 4009.589 kb on - strand, within BT3144 at 4009.610 kb on - strand, within BT3144 at 4009.612 kb on + strand, within BT3144 at 4009.644 kb on - strand, within BT3144 at 4009.659 kb on + strand, within BT3144 at 4009.667 kb on + strand at 4009.737 kb on - strand at 4009.750 kb on - strand at 4009.769 kb on - strand at 4009.769 kb on - strand at 4009.769 kb on - strand at 4009.791 kb on + strand at 4009.791 kb on + strand at 4009.792 kb on - strand at 4009.793 kb on + strand at 4009.794 kb on - strand at 4009.794 kb on - strand at 4009.796 kb on - strand at 4009.809 kb on - strand at 4009.813 kb on - strand at 4009.813 kb on - strand at 4009.813 kb on - strand at 4009.857 kb on - strand at 4009.857 kb on - strand at 4009.859 kb on + strand at 4009.861 kb on + strand at 4009.927 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 4,007,007 - BT3141 0.17 -0.7 4,007,007 - BT3141 0.17 +0.2 4,007,034 - BT3141 0.23 -0.4 4,007,040 - BT3141 0.24 +0.5 4,007,040 - +1.6 4,007,059 + BT3141 0.28 -3.4 4,007,114 + BT3141 0.40 -2.7 4,007,197 + BT3141 0.57 -1.4 4,007,449 + +0.6 4,007,486 - -2.4 4,007,567 - +0.8 4,007,585 - +0.4 4,007,601 - +0.6 4,007,601 - -3.7 4,007,696 - +1.6 4,007,747 - BT3142 0.38 -1.3 4,007,749 - -1.7 4,007,749 - BT3142 0.38 -3.0 4,007,894 + BT3142 0.73 +1.2 4,007,895 - BT3142 0.74 -1.2 4,007,895 - BT3142 0.74 +1.2 4,007,895 - BT3142 0.74 +0.6 4,007,896 + BT3142 0.74 +1.6 4,007,896 + BT3142 0.74 +0.0 4,007,897 - BT3142 0.74 -2.2 4,007,897 - BT3142 0.74 +1.1 4,007,995 - -1.6 4,008,005 - -0.4 4,008,005 - -0.9 4,008,013 + +1.6 4,008,049 - -1.9 4,008,149 - BT3143 0.16 +1.6 4,008,149 - BT3143 0.16 -1.6 4,008,237 - BT3143 0.25 -1.7 4,008,237 - BT3143 0.25 +0.6 4,008,242 - BT3143 0.25 +0.7 4,008,252 + BT3143 0.26 +0.6 4,008,253 - BT3143 0.27 -4.5 4,008,253 - BT3143 0.27 -0.7 4,008,276 - BT3143 0.29 -2.9 4,008,337 - BT3143 0.35 -1.5 4,008,337 - BT3143 0.35 -1.3 4,008,427 - BT3143 0.45 -0.1 4,008,439 - BT3143 0.46 +0.6 4,008,459 - BT3143 0.48 -1.6 4,008,462 - +0.6 4,008,476 - BT3143 0.50 -0.0 4,008,519 - BT3143 0.55 -0.4 4,008,657 - -0.0 4,008,657 - BT3143 0.69 -0.5 4,008,737 - BT3143 0.77 -2.9 4,008,941 + -0.7 4,008,985 + -3.3 4,009,105 + BT3144 0.14 -0.0 4,009,105 + BT3144 0.14 -0.7 4,009,106 - BT3144 0.15 +1.6 4,009,106 - BT3144 0.15 +0.2 4,009,149 + BT3144 0.20 -0.0 4,009,185 + BT3144 0.25 -2.7 4,009,195 + BT3144 0.27 +0.3 4,009,196 - BT3144 0.27 -1.2 4,009,274 - BT3144 0.37 -0.9 4,009,339 + BT3144 0.46 +1.0 4,009,342 + BT3144 0.46 +1.5 4,009,359 + BT3144 0.49 -0.6 4,009,499 + BT3144 0.68 -3.1 4,009,499 + +1.6 4,009,499 + BT3144 0.68 +1.4 4,009,579 + BT3144 0.78 -1.4 4,009,588 + BT3144 0.80 -1.7 4,009,589 - BT3144 0.80 +0.4 4,009,610 - BT3144 0.83 +0.9 4,009,612 + BT3144 0.83 -0.4 4,009,644 - BT3144 0.87 -0.8 4,009,659 + BT3144 0.89 -1.7 4,009,667 + +0.6 4,009,737 - -0.0 4,009,750 - +1.3 4,009,769 - +0.4 4,009,769 - -0.0 4,009,769 - +0.2 4,009,791 + -0.3 4,009,791 + -1.3 4,009,792 - -3.3 4,009,793 + -0.2 4,009,794 - -0.6 4,009,794 - -3.1 4,009,796 - -1.6 4,009,809 - +1.6 4,009,813 - +1.6 4,009,813 - -0.5 4,009,813 - +2.1 4,009,857 - +0.8 4,009,857 - +0.1 4,009,859 + -2.1 4,009,861 + -2.6 4,009,927 + +1.4
Or see this region's nucleotide sequence