Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2573 and BT2574 are separated by 66 nucleotides BT2574 and tRNA-Phe are separated by 205 nucleotides tRNA-Phe and BT2575 are separated by 234 nucleotides BT2575 and BT2576 overlap by 4 nucleotides
BT2573: BT2573 - glutamate/gamma-aminobutyrate anti-porter (NCBI ptt file), at 3,211,163 to 3,212,875
BT2573
BT2574: BT2574 - two-component system sensor histidine kinase (NCBI ptt file), at 3,212,942 to 3,214,201
BT2574
BT_t40: tRNA-Phe - tRNA-Phe (RefSeq), at 3,214,407 to 3,214,482
tRNA-Phe
BT2575: BT2575 - hypothetical protein (NCBI ptt file), at 3,214,717 to 3,214,938
BT2575
BT2576: BT2576 - transposase (NCBI ptt file), at 3,214,935 to 3,216,170
BT2576
Position (kb)
3212
3213
3214
3215 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3211.949 kb on - strand, within BT2573 at 3211.955 kb on - strand, within BT2573 at 3211.955 kb on - strand, within BT2573 at 3211.958 kb on - strand, within BT2573 at 3211.958 kb on - strand, within BT2573 at 3211.963 kb on - strand, within BT2573 at 3211.991 kb on - strand, within BT2573 at 3211.995 kb on - strand, within BT2573 at 3211.995 kb on - strand, within BT2573 at 3212.116 kb on + strand, within BT2573 at 3212.117 kb on - strand, within BT2573 at 3212.117 kb on - strand, within BT2573 at 3212.126 kb on + strand, within BT2573 at 3212.126 kb on + strand, within BT2573 at 3212.126 kb on + strand, within BT2573 at 3212.128 kb on + strand, within BT2573 at 3212.128 kb on + strand, within BT2573 at 3212.129 kb on - strand, within BT2573 at 3212.129 kb on - strand, within BT2573 at 3212.285 kb on + strand, within BT2573 at 3212.294 kb on - strand, within BT2573 at 3212.294 kb on - strand, within BT2573 at 3212.294 kb on - strand, within BT2573 at 3212.333 kb on + strand, within BT2573 at 3212.333 kb on + strand, within BT2573 at 3212.335 kb on + strand, within BT2573 at 3212.335 kb on + strand, within BT2573 at 3212.524 kb on - strand, within BT2573 at 3212.546 kb on + strand, within BT2573 at 3212.547 kb on - strand, within BT2573 at 3212.547 kb on - strand, within BT2573 at 3212.571 kb on + strand, within BT2573 at 3212.626 kb on + strand, within BT2573 at 3212.638 kb on + strand, within BT2573 at 3212.729 kb on - strand at 3212.762 kb on - strand at 3212.762 kb on - strand at 3212.849 kb on - strand at 3212.886 kb on + strand at 3212.887 kb on - strand at 3212.893 kb on + strand at 3212.893 kb on + strand at 3212.894 kb on - strand at 3212.894 kb on - strand at 3212.897 kb on + strand at 3212.939 kb on + strand at 3212.940 kb on - strand at 3212.945 kb on + strand at 3212.950 kb on + strand at 3212.950 kb on + strand at 3212.961 kb on - strand at 3213.001 kb on + strand at 3213.026 kb on + strand at 3213.050 kb on - strand at 3213.177 kb on + strand, within BT2574 at 3213.177 kb on + strand, within BT2574 at 3213.183 kb on + strand, within BT2574 at 3213.253 kb on + strand, within BT2574 at 3213.272 kb on + strand, within BT2574 at 3213.283 kb on + strand, within BT2574 at 3213.497 kb on - strand, within BT2574 at 3213.508 kb on + strand, within BT2574 at 3213.519 kb on + strand, within BT2574 at 3213.520 kb on - strand, within BT2574 at 3213.520 kb on - strand, within BT2574 at 3213.610 kb on + strand, within BT2574 at 3213.685 kb on + strand, within BT2574 at 3213.709 kb on - strand, within BT2574 at 3213.709 kb on - strand, within BT2574 at 3213.724 kb on + strand, within BT2574 at 3213.750 kb on + strand, within BT2574 at 3213.751 kb on - strand, within BT2574 at 3213.814 kb on + strand, within BT2574 at 3213.863 kb on + strand, within BT2574 at 3213.865 kb on + strand, within BT2574 at 3213.871 kb on + strand, within BT2574 at 3213.871 kb on + strand, within BT2574 at 3213.871 kb on + strand, within BT2574 at 3213.872 kb on - strand, within BT2574 at 3213.875 kb on + strand, within BT2574 at 3213.875 kb on + strand, within BT2574 at 3213.875 kb on + strand, within BT2574 at 3213.876 kb on - strand, within BT2574 at 3213.878 kb on - strand, within BT2574 at 3213.878 kb on - strand, within BT2574 at 3213.890 kb on - strand, within BT2574 at 3213.997 kb on + strand, within BT2574 at 3213.999 kb on + strand, within BT2574 at 3213.999 kb on + strand, within BT2574 at 3214.003 kb on - strand, within BT2574 at 3214.045 kb on - strand, within BT2574 at 3214.067 kb on + strand, within BT2574 at 3214.068 kb on - strand, within BT2574 at 3214.100 kb on + strand at 3214.101 kb on - strand at 3214.144 kb on + strand at 3214.145 kb on - strand at 3214.145 kb on - strand at 3214.172 kb on + strand at 3214.204 kb on + strand at 3214.204 kb on + strand at 3214.204 kb on + strand at 3214.209 kb on + strand at 3214.210 kb on - strand at 3214.214 kb on + strand at 3214.215 kb on - strand at 3214.279 kb on + strand at 3214.279 kb on + strand at 3214.280 kb on - strand at 3214.354 kb on - strand at 3214.383 kb on - strand at 3214.563 kb on - strand at 3214.652 kb on + strand at 3214.715 kb on + strand at 3214.716 kb on - strand at 3214.769 kb on - strand, within BT2575 at 3214.797 kb on + strand, within BT2575 at 3214.908 kb on + strand, within BT2575 at 3215.006 kb on + strand at 3215.007 kb on - strand at 3215.038 kb on - strand at 3215.100 kb on - strand, within BT2576 at 3215.102 kb on + strand, within BT2576 at 3215.103 kb on - strand, within BT2576 at 3215.139 kb on + strand, within BT2576 at 3215.143 kb on + strand, within BT2576 at 3215.186 kb on + strand, within BT2576 at 3215.186 kb on + strand, within BT2576 at 3215.189 kb on - strand, within BT2576
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 3,211,949 - BT2573 0.46 +0.5 3,211,955 - BT2573 0.46 +3.7 3,211,955 - BT2573 0.46 -3.7 3,211,958 - BT2573 0.46 -1.8 3,211,958 - BT2573 0.46 -0.3 3,211,963 - BT2573 0.47 -0.4 3,211,991 - BT2573 0.48 +0.6 3,211,995 - BT2573 0.49 +0.7 3,211,995 - BT2573 0.49 +1.2 3,212,116 + BT2573 0.56 +0.3 3,212,117 - BT2573 0.56 -2.1 3,212,117 - BT2573 0.56 +0.7 3,212,126 + BT2573 0.56 -0.3 3,212,126 + BT2573 0.56 +0.6 3,212,126 + BT2573 0.56 +1.4 3,212,128 + BT2573 0.56 +0.3 3,212,128 + BT2573 0.56 -1.8 3,212,129 - BT2573 0.56 +0.5 3,212,129 - BT2573 0.56 +0.3 3,212,285 + BT2573 0.65 +2.4 3,212,294 - BT2573 0.66 +0.6 3,212,294 - BT2573 0.66 +1.0 3,212,294 - BT2573 0.66 -0.4 3,212,333 + BT2573 0.68 +0.4 3,212,333 + BT2573 0.68 -0.5 3,212,335 + BT2573 0.68 +3.0 3,212,335 + BT2573 0.68 +0.3 3,212,524 - BT2573 0.79 +0.9 3,212,546 + BT2573 0.81 -3.9 3,212,547 - BT2573 0.81 -3.1 3,212,547 - BT2573 0.81 +0.4 3,212,571 + BT2573 0.82 -0.5 3,212,626 + BT2573 0.85 -1.8 3,212,638 + BT2573 0.86 +0.3 3,212,729 - -2.6 3,212,762 - -1.7 3,212,762 - -2.6 3,212,849 - +0.8 3,212,886 + +0.4 3,212,887 - -3.2 3,212,893 + -1.1 3,212,893 + -1.8 3,212,894 - -0.9 3,212,894 - -1.7 3,212,897 + -0.2 3,212,939 + -0.9 3,212,940 - +0.1 3,212,945 + +2.0 3,212,950 + -0.2 3,212,950 + -1.5 3,212,961 - +1.4 3,213,001 + -3.2 3,213,026 + -1.0 3,213,050 - -0.9 3,213,177 + BT2574 0.19 -0.6 3,213,177 + BT2574 0.19 -0.7 3,213,183 + BT2574 0.19 -2.7 3,213,253 + BT2574 0.25 -0.0 3,213,272 + BT2574 0.26 -2.4 3,213,283 + BT2574 0.27 -2.4 3,213,497 - BT2574 0.44 -2.5 3,213,508 + BT2574 0.45 -0.9 3,213,519 + BT2574 0.46 -0.6 3,213,520 - BT2574 0.46 -3.6 3,213,520 - BT2574 0.46 +0.2 3,213,610 + BT2574 0.53 +0.4 3,213,685 + BT2574 0.59 -2.8 3,213,709 - BT2574 0.61 -0.2 3,213,709 - BT2574 0.61 +0.1 3,213,724 + BT2574 0.62 -0.2 3,213,750 + BT2574 0.64 -0.8 3,213,751 - BT2574 0.64 -0.7 3,213,814 + BT2574 0.69 +1.7 3,213,863 + BT2574 0.73 +0.0 3,213,865 + BT2574 0.73 -3.4 3,213,871 + BT2574 0.74 -1.4 3,213,871 + BT2574 0.74 -1.4 3,213,871 + BT2574 0.74 -2.8 3,213,872 - BT2574 0.74 -2.7 3,213,875 + BT2574 0.74 -2.0 3,213,875 + BT2574 0.74 -2.7 3,213,875 + BT2574 0.74 -0.2 3,213,876 - BT2574 0.74 +0.3 3,213,878 - BT2574 0.74 -1.3 3,213,878 - BT2574 0.74 -0.1 3,213,890 - BT2574 0.75 -1.2 3,213,997 + BT2574 0.84 -2.5 3,213,999 + BT2574 0.84 +0.5 3,213,999 + BT2574 0.84 -0.5 3,214,003 - BT2574 0.84 +0.9 3,214,045 - BT2574 0.88 -1.9 3,214,067 + BT2574 0.89 -3.5 3,214,068 - BT2574 0.89 -1.6 3,214,100 + -0.6 3,214,101 - -2.2 3,214,144 + +0.5 3,214,145 - +0.2 3,214,145 - -1.6 3,214,172 + -1.6 3,214,204 + -3.3 3,214,204 + -2.5 3,214,204 + -0.2 3,214,209 + -0.6 3,214,210 - -0.5 3,214,214 + +2.4 3,214,215 - -1.1 3,214,279 + -1.2 3,214,279 + -3.0 3,214,280 - -1.0 3,214,354 - -2.0 3,214,383 - -0.6 3,214,563 - +0.4 3,214,652 + +2.0 3,214,715 + +0.1 3,214,716 - -3.1 3,214,769 - BT2575 0.23 -1.9 3,214,797 + BT2575 0.36 +0.1 3,214,908 + BT2575 0.86 -3.0 3,215,006 + +0.2 3,215,007 - -2.3 3,215,038 - +0.1 3,215,100 - BT2576 0.13 -1.2 3,215,102 + BT2576 0.14 -0.8 3,215,103 - BT2576 0.14 +0.3 3,215,139 + BT2576 0.17 -0.9 3,215,143 + BT2576 0.17 -0.8 3,215,186 + BT2576 0.20 -0.0 3,215,186 + BT2576 0.20 -1.2 3,215,189 - BT2576 0.21 -2.3
Or see this region's nucleotide sequence