Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2113 and BT2114 are separated by 118 nucleotides BT2114 and BT2115 are separated by 163 nucleotides BT2115 and BT2116 are separated by 78 nucleotides
BT2113: BT2113 - conserved hypothetical protein (NCBI ptt file), at 2,657,209 to 2,660,634
BT2113
BT2114: BT2114 - hypothetical protein (NCBI ptt file), at 2,660,753 to 2,661,529
BT2114
BT2115: BT2115 - hypothetical protein (NCBI ptt file), at 2,661,693 to 2,661,905
BT2115
BT2116: BT2116 - conserved hypothetical protein (NCBI ptt file), at 2,661,984 to 2,662,535
BT2116
Position (kb)
2660
2661
2662 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2659.788 kb on + strand, within BT2113 at 2659.788 kb on + strand, within BT2113 at 2659.793 kb on - strand, within BT2113 at 2659.801 kb on - strand, within BT2113 at 2659.867 kb on + strand, within BT2113 at 2659.939 kb on + strand, within BT2113 at 2660.004 kb on + strand, within BT2113 at 2660.005 kb on - strand, within BT2113 at 2660.005 kb on - strand, within BT2113 at 2660.005 kb on - strand, within BT2113 at 2660.006 kb on + strand, within BT2113 at 2660.037 kb on + strand, within BT2113 at 2660.037 kb on + strand, within BT2113 at 2660.038 kb on - strand, within BT2113 at 2660.094 kb on + strand, within BT2113 at 2660.129 kb on + strand, within BT2113 at 2660.138 kb on + strand, within BT2113 at 2660.139 kb on - strand, within BT2113 at 2660.158 kb on + strand, within BT2113 at 2660.170 kb on + strand, within BT2113 at 2660.188 kb on + strand, within BT2113 at 2660.205 kb on + strand, within BT2113 at 2660.210 kb on - strand, within BT2113 at 2660.290 kb on - strand, within BT2113 at 2660.326 kb on - strand at 2660.334 kb on - strand at 2660.335 kb on + strand at 2660.335 kb on + strand at 2660.431 kb on - strand at 2660.452 kb on + strand at 2660.453 kb on - strand at 2660.455 kb on - strand at 2660.460 kb on - strand at 2660.477 kb on + strand at 2660.477 kb on + strand at 2660.587 kb on - strand at 2660.614 kb on - strand at 2660.653 kb on + strand at 2660.654 kb on - strand at 2660.729 kb on - strand at 2660.732 kb on - strand at 2660.734 kb on + strand at 2660.778 kb on - strand at 2660.853 kb on - strand, within BT2114 at 2660.991 kb on - strand, within BT2114 at 2661.056 kb on + strand, within BT2114 at 2661.057 kb on - strand, within BT2114 at 2661.062 kb on - strand, within BT2114 at 2661.124 kb on - strand, within BT2114 at 2661.144 kb on + strand at 2661.145 kb on - strand, within BT2114 at 2661.267 kb on - strand at 2661.296 kb on + strand, within BT2114 at 2661.296 kb on + strand, within BT2114 at 2661.297 kb on - strand, within BT2114 at 2661.297 kb on - strand, within BT2114 at 2661.300 kb on + strand, within BT2114 at 2661.301 kb on - strand, within BT2114 at 2661.301 kb on - strand, within BT2114 at 2661.302 kb on + strand, within BT2114 at 2661.303 kb on - strand, within BT2114 at 2661.326 kb on - strand, within BT2114 at 2661.373 kb on - strand, within BT2114 at 2661.550 kb on - strand at 2661.570 kb on + strand at 2661.571 kb on - strand at 2661.576 kb on - strand at 2661.578 kb on + strand at 2661.578 kb on + strand at 2661.585 kb on - strand at 2661.604 kb on - strand at 2661.605 kb on + strand at 2661.608 kb on + strand at 2661.611 kb on - strand at 2661.642 kb on + strand at 2661.668 kb on + strand at 2661.668 kb on + strand at 2661.670 kb on + strand at 2661.693 kb on - strand at 2661.710 kb on + strand at 2661.729 kb on + strand, within BT2115 at 2661.730 kb on - strand, within BT2115 at 2661.803 kb on - strand, within BT2115 at 2661.803 kb on - strand, within BT2115 at 2661.858 kb on - strand, within BT2115 at 2661.897 kb on - strand at 2661.899 kb on + strand at 2661.904 kb on - strand at 2661.917 kb on + strand at 2661.953 kb on + strand at 2661.960 kb on + strand at 2661.961 kb on - strand at 2661.962 kb on + strand at 2661.963 kb on - strand at 2661.968 kb on + strand at 2661.995 kb on + strand at 2662.076 kb on - strand, within BT2116 at 2662.142 kb on + strand, within BT2116 at 2662.157 kb on + strand, within BT2116 at 2662.195 kb on - strand, within BT2116 at 2662.237 kb on + strand, within BT2116 at 2662.508 kb on - strand at 2662.508 kb on - strand at 2662.508 kb on - strand at 2662.508 kb on - strand at 2662.521 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 2,659,788 + BT2113 0.75 -2.6 2,659,788 + BT2113 0.75 +1.8 2,659,793 - BT2113 0.75 -1.2 2,659,801 - BT2113 0.76 +0.4 2,659,867 + BT2113 0.78 +0.4 2,659,939 + BT2113 0.80 -0.7 2,660,004 + BT2113 0.82 +0.4 2,660,005 - BT2113 0.82 +1.2 2,660,005 - BT2113 0.82 +2.4 2,660,005 - BT2113 0.82 -2.7 2,660,006 + BT2113 0.82 +2.4 2,660,037 + BT2113 0.83 +0.4 2,660,037 + BT2113 0.83 +1.4 2,660,038 - BT2113 0.83 -1.6 2,660,094 + BT2113 0.84 -1.6 2,660,129 + BT2113 0.85 -0.3 2,660,138 + BT2113 0.85 -1.6 2,660,139 - BT2113 0.86 -1.2 2,660,158 + BT2113 0.86 -0.8 2,660,170 + BT2113 0.86 -1.7 2,660,188 + BT2113 0.87 +1.8 2,660,205 + BT2113 0.87 -1.5 2,660,210 - BT2113 0.88 -0.2 2,660,290 - BT2113 0.90 +0.8 2,660,326 - +0.1 2,660,334 - +0.8 2,660,335 + +0.0 2,660,335 + -0.2 2,660,431 - +0.1 2,660,452 + -0.2 2,660,453 - -1.6 2,660,455 - +1.9 2,660,460 - -0.0 2,660,477 + -2.5 2,660,477 + -0.7 2,660,587 - -0.0 2,660,614 - +2.1 2,660,653 + -2.8 2,660,654 - -0.2 2,660,729 - -0.4 2,660,732 - +0.4 2,660,734 + +3.4 2,660,778 - +0.7 2,660,853 - BT2114 0.13 +1.2 2,660,991 - BT2114 0.31 +0.4 2,661,056 + BT2114 0.39 +2.0 2,661,057 - BT2114 0.39 +0.4 2,661,062 - BT2114 0.40 -2.9 2,661,124 - BT2114 0.48 -0.2 2,661,144 + +1.4 2,661,145 - BT2114 0.50 -0.6 2,661,267 - +3.4 2,661,296 + BT2114 0.70 +0.7 2,661,296 + BT2114 0.70 -2.1 2,661,297 - BT2114 0.70 +0.8 2,661,297 - BT2114 0.70 +1.6 2,661,300 + BT2114 0.70 -3.7 2,661,301 - BT2114 0.71 +1.9 2,661,301 - BT2114 0.71 -0.6 2,661,302 + BT2114 0.71 -1.1 2,661,303 - BT2114 0.71 -2.3 2,661,326 - BT2114 0.74 -0.6 2,661,373 - BT2114 0.80 +1.6 2,661,550 - +0.7 2,661,570 + +0.2 2,661,571 - -0.4 2,661,576 - -0.6 2,661,578 + -1.5 2,661,578 + -2.0 2,661,585 - -1.4 2,661,604 - +2.0 2,661,605 + -1.7 2,661,608 + +0.9 2,661,611 - +0.5 2,661,642 + +0.6 2,661,668 + -3.0 2,661,668 + +1.4 2,661,670 + -1.5 2,661,693 - -3.3 2,661,710 + +0.6 2,661,729 + BT2115 0.17 -1.1 2,661,730 - BT2115 0.17 -1.5 2,661,803 - BT2115 0.52 +0.6 2,661,803 - BT2115 0.52 -0.2 2,661,858 - BT2115 0.77 +0.0 2,661,897 - -2.6 2,661,899 + -2.1 2,661,904 - -1.6 2,661,917 + +0.0 2,661,953 + -0.5 2,661,960 + -1.1 2,661,961 - -0.0 2,661,962 + -1.8 2,661,963 - +0.8 2,661,968 + -1.5 2,661,995 + -0.2 2,662,076 - BT2116 0.17 -2.3 2,662,142 + BT2116 0.29 -2.5 2,662,157 + BT2116 0.31 -0.8 2,662,195 - BT2116 0.38 -1.2 2,662,237 + BT2116 0.46 +0.1 2,662,508 - -0.2 2,662,508 - -2.2 2,662,508 - +0.5 2,662,508 - -2.9 2,662,521 + -0.2
Or see this region's nucleotide sequence