Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1300 and BT1301 are separated by 11 nucleotides BT1301 and BT1302 are separated by 224 nucleotides BT1302 and BT1303 are separated by 17 nucleotides BT1303 and BT1304 are separated by 14 nucleotides
BT1300: BT1300 - hypothetical protein (NCBI ptt file), at 1,621,178 to 1,622,020
BT1300
BT1301: BT1301 - ATP synthase subunit E (NCBI ptt file), at 1,622,032 to 1,622,622
BT1301
BT1302: BT1302 - hypothetical protein (NCBI ptt file), at 1,622,847 to 1,623,293
BT1302
BT1303: BT1303 - hypothetical protein (NCBI ptt file), at 1,623,311 to 1,624,111
BT1303
BT1304: BT1304 - putative transcriptional regulator (NCBI ptt file), at 1,624,126 to 1,624,497
BT1304
Position (kb)
1622
1623
1624 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1621.948 kb on + strand at 1622.001 kb on + strand at 1622.004 kb on + strand at 1622.005 kb on - strand at 1622.007 kb on + strand at 1622.011 kb on + strand at 1622.078 kb on + strand at 1622.079 kb on - strand at 1622.256 kb on + strand, within BT1301 at 1622.257 kb on - strand, within BT1301 at 1622.647 kb on + strand at 1622.652 kb on + strand at 1622.760 kb on + strand at 1622.826 kb on - strand at 1622.870 kb on - strand at 1622.894 kb on + strand, within BT1302 at 1622.913 kb on - strand, within BT1302 at 1622.939 kb on + strand, within BT1302 at 1622.940 kb on - strand, within BT1302 at 1622.940 kb on - strand, within BT1302 at 1622.948 kb on - strand, within BT1302 at 1622.951 kb on + strand, within BT1302 at 1622.970 kb on - strand, within BT1302 at 1623.009 kb on - strand, within BT1302 at 1623.121 kb on - strand, within BT1302 at 1623.192 kb on - strand, within BT1302 at 1623.202 kb on - strand, within BT1302 at 1623.237 kb on - strand, within BT1302 at 1623.237 kb on - strand, within BT1302 at 1623.306 kb on + strand at 1623.324 kb on - strand at 1623.325 kb on + strand at 1623.383 kb on + strand at 1623.391 kb on + strand, within BT1303 at 1623.406 kb on + strand, within BT1303 at 1623.406 kb on + strand, within BT1303 at 1623.421 kb on + strand, within BT1303 at 1623.421 kb on + strand, within BT1303 at 1623.445 kb on - strand, within BT1303 at 1623.507 kb on + strand, within BT1303 at 1623.653 kb on - strand, within BT1303 at 1623.655 kb on + strand, within BT1303 at 1623.686 kb on + strand, within BT1303 at 1623.687 kb on - strand, within BT1303 at 1623.771 kb on + strand, within BT1303 at 1623.775 kb on + strand, within BT1303 at 1623.806 kb on + strand, within BT1303 at 1623.862 kb on + strand, within BT1303 at 1623.863 kb on - strand, within BT1303 at 1623.906 kb on - strand, within BT1303 at 1623.915 kb on - strand, within BT1303 at 1623.925 kb on + strand, within BT1303 at 1623.926 kb on - strand, within BT1303 at 1624.056 kb on - strand at 1624.091 kb on + strand at 1624.095 kb on + strand at 1624.112 kb on - strand at 1624.116 kb on + strand at 1624.144 kb on + strand at 1624.196 kb on + strand, within BT1304
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 1,621,948 + -0.1 1,622,001 + +0.6 1,622,004 + +0.1 1,622,005 - +1.1 1,622,007 + +0.4 1,622,011 + -1.0 1,622,078 + -0.6 1,622,079 - +0.4 1,622,256 + BT1301 0.38 +2.1 1,622,257 - BT1301 0.38 -2.2 1,622,647 + -2.2 1,622,652 + +0.4 1,622,760 + -0.2 1,622,826 - -1.4 1,622,870 - -2.7 1,622,894 + BT1302 0.11 +3.0 1,622,913 - BT1302 0.15 -1.2 1,622,939 + BT1302 0.21 -1.6 1,622,940 - BT1302 0.21 +0.9 1,622,940 - BT1302 0.21 +0.7 1,622,948 - BT1302 0.23 +1.1 1,622,951 + BT1302 0.23 -1.5 1,622,970 - BT1302 0.28 +2.5 1,623,009 - BT1302 0.36 -0.5 1,623,121 - BT1302 0.61 -4.2 1,623,192 - BT1302 0.77 -2.1 1,623,202 - BT1302 0.79 -2.5 1,623,237 - BT1302 0.87 +1.4 1,623,237 - BT1302 0.87 -0.8 1,623,306 + -2.3 1,623,324 - -0.1 1,623,325 + -0.4 1,623,383 + -1.2 1,623,391 + BT1303 0.10 -0.3 1,623,406 + BT1303 0.12 -0.4 1,623,406 + BT1303 0.12 -2.3 1,623,421 + BT1303 0.14 +0.7 1,623,421 + BT1303 0.14 -0.2 1,623,445 - BT1303 0.17 +0.8 1,623,507 + BT1303 0.24 -0.9 1,623,653 - BT1303 0.43 -1.5 1,623,655 + BT1303 0.43 -1.2 1,623,686 + BT1303 0.47 -3.5 1,623,687 - BT1303 0.47 +2.6 1,623,771 + BT1303 0.57 +0.2 1,623,775 + BT1303 0.58 -2.8 1,623,806 + BT1303 0.62 -0.3 1,623,862 + BT1303 0.69 +0.4 1,623,863 - BT1303 0.69 -2.6 1,623,906 - BT1303 0.74 +1.4 1,623,915 - BT1303 0.75 -2.8 1,623,925 + BT1303 0.77 -2.7 1,623,926 - BT1303 0.77 +2.9 1,624,056 - -2.8 1,624,091 + -1.6 1,624,095 + +0.6 1,624,112 - -2.1 1,624,116 + +0.3 1,624,144 + +3.1 1,624,196 + BT1304 0.19 +0.9
Or see this region's nucleotide sequence