Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1291 and BT1292 are separated by 139 nucleotides BT1292 and BT1293 are separated by 335 nucleotides
BT1291: BT1291 - spermidine/putrescine transport ATP-binding protein (NCBI ptt file), at 1,607,626 to 1,609,017
BT1291
BT1292: BT1292 - thiol:disulfide interchange protein (NCBI ptt file), at 1,609,157 to 1,610,257
BT1292
BT1293: BT1293 - alpha-glucan phosphorylase (NCBI ptt file), at 1,610,593 to 1,613,157
BT1293
Position (kb)
1609
1610
1611 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1608.335 kb on + strand, within BT1291 at 1608.434 kb on + strand, within BT1291 at 1608.434 kb on + strand, within BT1291 at 1608.437 kb on - strand, within BT1291 at 1608.444 kb on + strand, within BT1291 at 1608.445 kb on - strand, within BT1291 at 1608.570 kb on + strand, within BT1291 at 1608.724 kb on + strand, within BT1291 at 1608.749 kb on + strand, within BT1291 at 1608.750 kb on - strand, within BT1291 at 1608.973 kb on + strand at 1609.017 kb on + strand at 1609.071 kb on + strand at 1609.077 kb on - strand at 1609.132 kb on - strand at 1609.135 kb on + strand at 1609.180 kb on + strand at 1609.232 kb on + strand at 1609.449 kb on + strand, within BT1292 at 1609.516 kb on + strand, within BT1292 at 1609.516 kb on + strand, within BT1292 at 1609.517 kb on - strand, within BT1292 at 1609.517 kb on - strand, within BT1292 at 1609.649 kb on - strand, within BT1292 at 1609.653 kb on + strand, within BT1292 at 1609.653 kb on + strand, within BT1292 at 1609.699 kb on - strand, within BT1292 at 1609.699 kb on - strand, within BT1292 at 1609.726 kb on + strand, within BT1292 at 1609.840 kb on - strand at 1609.844 kb on - strand, within BT1292 at 1609.846 kb on + strand, within BT1292 at 1609.847 kb on - strand, within BT1292 at 1609.918 kb on + strand, within BT1292 at 1609.978 kb on + strand, within BT1292 at 1609.988 kb on + strand, within BT1292 at 1609.994 kb on + strand, within BT1292 at 1610.086 kb on + strand, within BT1292 at 1610.144 kb on + strand, within BT1292 at 1610.191 kb on + strand at 1610.195 kb on - strand at 1610.195 kb on - strand at 1610.283 kb on - strand at 1610.480 kb on - strand at 1610.480 kb on - strand at 1610.491 kb on + strand at 1610.546 kb on + strand at 1610.592 kb on - strand at 1610.698 kb on - strand at 1610.763 kb on - strand at 1610.790 kb on + strand at 1610.792 kb on + strand at 1610.797 kb on - strand at 1610.797 kb on - strand at 1610.850 kb on - strand, within BT1293 at 1610.853 kb on + strand, within BT1293 at 1610.857 kb on - strand, within BT1293 at 1610.857 kb on - strand, within BT1293 at 1610.857 kb on - strand, within BT1293 at 1610.859 kb on + strand, within BT1293 at 1610.859 kb on + strand, within BT1293 at 1610.861 kb on + strand, within BT1293 at 1610.917 kb on + strand, within BT1293 at 1611.028 kb on - strand, within BT1293 at 1611.039 kb on + strand, within BT1293 at 1611.040 kb on - strand, within BT1293 at 1611.097 kb on - strand, within BT1293 at 1611.097 kb on - strand, within BT1293 at 1611.153 kb on - strand, within BT1293 at 1611.153 kb on - strand, within BT1293 at 1611.184 kb on - strand, within BT1293 at 1611.184 kb on - strand, within BT1293 at 1611.192 kb on + strand, within BT1293 at 1611.193 kb on - strand, within BT1293 at 1611.193 kb on - strand, within BT1293 at 1611.234 kb on - strand, within BT1293
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 1,608,335 + BT1291 0.51 -1.6 1,608,434 + BT1291 0.58 -2.5 1,608,434 + BT1291 0.58 +0.4 1,608,437 - BT1291 0.58 -2.1 1,608,444 + BT1291 0.59 +0.2 1,608,445 - BT1291 0.59 -0.6 1,608,570 + BT1291 0.68 -0.5 1,608,724 + BT1291 0.79 -2.1 1,608,749 + BT1291 0.81 -0.6 1,608,750 - BT1291 0.81 -3.3 1,608,973 + -1.6 1,609,017 + -0.6 1,609,071 + -1.0 1,609,077 - -3.5 1,609,132 - +0.1 1,609,135 + -0.6 1,609,180 + -1.1 1,609,232 + -2.8 1,609,449 + BT1292 0.27 -1.8 1,609,516 + BT1292 0.33 -3.1 1,609,516 + BT1292 0.33 -2.5 1,609,517 - BT1292 0.33 +0.0 1,609,517 - BT1292 0.33 -1.1 1,609,649 - BT1292 0.45 -1.0 1,609,653 + BT1292 0.45 -2.5 1,609,653 + BT1292 0.45 +1.4 1,609,699 - BT1292 0.49 -0.2 1,609,699 - BT1292 0.49 -1.0 1,609,726 + BT1292 0.52 -1.6 1,609,840 - +1.4 1,609,844 - BT1292 0.62 -0.4 1,609,846 + BT1292 0.63 -2.0 1,609,847 - BT1292 0.63 -2.1 1,609,918 + BT1292 0.69 +0.4 1,609,978 + BT1292 0.75 +1.4 1,609,988 + BT1292 0.75 -1.0 1,609,994 + BT1292 0.76 -3.0 1,610,086 + BT1292 0.84 -2.6 1,610,144 + BT1292 0.90 -0.2 1,610,191 + -2.7 1,610,195 - -2.8 1,610,195 - -1.0 1,610,283 - -1.3 1,610,480 - +1.4 1,610,480 - -3.8 1,610,491 + -1.2 1,610,546 + +0.4 1,610,592 - -1.5 1,610,698 - +1.9 1,610,763 - -3.5 1,610,790 + -2.8 1,610,792 + -1.1 1,610,797 - -3.5 1,610,797 - +1.4 1,610,850 - BT1293 0.10 -2.1 1,610,853 + BT1293 0.10 -2.5 1,610,857 - BT1293 0.10 -1.2 1,610,857 - BT1293 0.10 -2.1 1,610,857 - BT1293 0.10 -3.5 1,610,859 + BT1293 0.10 -1.2 1,610,859 + BT1293 0.10 -1.5 1,610,861 + BT1293 0.10 -1.2 1,610,917 + BT1293 0.13 -2.0 1,611,028 - BT1293 0.17 -1.8 1,611,039 + BT1293 0.17 -1.1 1,611,040 - BT1293 0.17 -1.8 1,611,097 - BT1293 0.20 -2.7 1,611,097 - BT1293 0.20 -0.6 1,611,153 - BT1293 0.22 +0.1 1,611,153 - BT1293 0.22 -0.5 1,611,184 - BT1293 0.23 -1.5 1,611,184 - BT1293 0.23 -1.5 1,611,192 + BT1293 0.23 -0.2 1,611,193 - BT1293 0.23 -2.5 1,611,193 - BT1293 0.23 -1.8 1,611,234 - BT1293 0.25 -2.6
Or see this region's nucleotide sequence