Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0744 and BT0745 overlap by 4 nucleotides BT0745 and BT0746 are separated by 3 nucleotides BT0746 and BT0747 are separated by 83 nucleotides
BT0744: BT0744 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 922,791 to 923,312
BT0744
BT0745: BT0745 - 3-deoxy-manno-octulosonate cytidylyltransferase (NCBI ptt file), at 923,309 to 924,061
BT0745
BT0746: BT0746 - putative zinc protease (NCBI ptt file), at 924,065 to 925,348
BT0746
BT0747: BT0747 - putative phosphatidylinositol-4-phosphate 5-kinase (NCBI ptt file), at 925,432 to 926,601
BT0747
Position (kb) 924
925
926 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 924.078 kb on + strand at 924.090 kb on + strand at 924.200 kb on - strand, within BT0746 at 924.320 kb on + strand, within BT0746 at 924.367 kb on + strand, within BT0746 at 924.463 kb on + strand, within BT0746 at 924.508 kb on + strand, within BT0746 at 924.645 kb on - strand, within BT0746 at 924.847 kb on + strand, within BT0746 at 924.847 kb on + strand, within BT0746 at 924.886 kb on - strand, within BT0746 at 924.937 kb on + strand, within BT0746 at 924.961 kb on + strand, within BT0746 at 925.136 kb on - strand, within BT0746 at 925.296 kb on - strand at 926.080 kb on + strand, within BT0747 at 926.214 kb on + strand, within BT0747 at 926.219 kb on - strand, within BT0747
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 924,078 + -1.2 924,090 + -1.4 924,200 - BT0746 0.11 +0.1 924,320 + BT0746 0.20 -0.6 924,367 + BT0746 0.24 -0.6 924,463 + BT0746 0.31 +1.4 924,508 + BT0746 0.35 +1.8 924,645 - BT0746 0.45 +0.4 924,847 + BT0746 0.61 +1.0 924,847 + BT0746 0.61 -1.6 924,886 - BT0746 0.64 -2.8 924,937 + BT0746 0.68 +3.1 924,961 + BT0746 0.70 +1.5 925,136 - BT0746 0.83 -1.6 925,296 - -2.1 926,080 + BT0747 0.55 -1.8 926,214 + BT0747 0.67 -3.3 926,219 - BT0747 0.67 -1.4
Or see this region's nucleotide sequence