Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0259 and BT0260 are separated by 122 nucleotides BT0260 and BT0261 are separated by 8 nucleotides BT0261 and BT0262 are separated by 357 nucleotides
BT0259: BT0259 - putative amidohydrolase (NCBI ptt file), at 290,470 to 291,255
BT0259
BT0260: BT0260 - acyltransferase family protein (NCBI ptt file), at 291,378 to 291,932
BT0260
BT0261: BT0261 - hypothetical protein (NCBI ptt file), at 291,941 to 292,210
BT0261
BT0262: BT0262 - hypothetical protein (NCBI ptt file), at 292,568 to 293,203
BT0262
Position (kb) 291
292
293 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 290.984 kb on + strand, within BT0259 at 290.985 kb on - strand, within BT0259 at 291.007 kb on - strand, within BT0259 at 291.009 kb on - strand, within BT0259 at 291.094 kb on - strand, within BT0259 at 291.133 kb on + strand, within BT0259 at 291.229 kb on + strand at 291.232 kb on + strand at 291.268 kb on - strand at 291.272 kb on - strand at 291.285 kb on + strand at 291.288 kb on + strand at 291.295 kb on + strand at 291.296 kb on - strand at 291.296 kb on - strand at 291.299 kb on + strand at 291.306 kb on + strand at 291.307 kb on - strand at 291.355 kb on - strand at 291.355 kb on - strand at 291.362 kb on - strand at 291.365 kb on + strand at 291.366 kb on - strand at 291.369 kb on - strand at 291.864 kb on - strand, within BT0260 at 292.010 kb on - strand, within BT0261 at 292.118 kb on - strand, within BT0261 at 292.136 kb on - strand, within BT0261 at 292.174 kb on - strand, within BT0261 at 292.174 kb on - strand, within BT0261 at 292.234 kb on - strand at 292.343 kb on + strand at 292.520 kb on - strand at 292.520 kb on - strand at 292.687 kb on - strand, within BT0262 at 292.687 kb on - strand, within BT0262 at 292.691 kb on - strand, within BT0262 at 292.760 kb on - strand, within BT0262 at 292.915 kb on - strand, within BT0262 at 292.955 kb on - strand, within BT0262 at 292.955 kb on - strand, within BT0262 at 292.957 kb on - strand, within BT0262 at 292.957 kb on - strand, within BT0262 at 292.960 kb on + strand, within BT0262 at 293.006 kb on + strand, within BT0262 at 293.008 kb on + strand, within BT0262 at 293.053 kb on - strand, within BT0262 at 293.053 kb on - strand, within BT0262 at 293.062 kb on + strand, within BT0262 at 293.103 kb on + strand, within BT0262 at 293.126 kb on - strand, within BT0262 at 293.126 kb on - strand, within BT0262 at 293.128 kb on - strand, within BT0262 at 293.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 290,984 + BT0259 0.65 +1.6 290,985 - BT0259 0.66 -2.0 291,007 - BT0259 0.68 -0.2 291,009 - BT0259 0.69 -1.6 291,094 - BT0259 0.79 -0.4 291,133 + BT0259 0.84 -0.4 291,229 + +0.1 291,232 + +0.6 291,268 - -2.3 291,272 - -2.9 291,285 + +0.2 291,288 + -1.2 291,295 + -1.4 291,296 - -2.2 291,296 - -2.1 291,299 + -1.2 291,306 + -1.6 291,307 - +0.6 291,355 - +0.1 291,355 - -2.0 291,362 - -0.3 291,365 + -1.2 291,366 - -2.4 291,369 - +0.4 291,864 - BT0260 0.88 -1.4 292,010 - BT0261 0.26 -3.0 292,118 - BT0261 0.66 -1.4 292,136 - BT0261 0.72 -3.1 292,174 - BT0261 0.86 +0.6 292,174 - BT0261 0.86 -1.1 292,234 - -2.5 292,343 + -2.2 292,520 - -0.3 292,520 - -1.7 292,687 - BT0262 0.19 +0.9 292,687 - BT0262 0.19 +0.5 292,691 - BT0262 0.19 +1.1 292,760 - BT0262 0.30 +0.4 292,915 - BT0262 0.55 -2.4 292,955 - BT0262 0.61 -1.6 292,955 - BT0262 0.61 +0.5 292,957 - BT0262 0.61 +0.7 292,957 - BT0262 0.61 +1.4 292,960 + BT0262 0.62 -2.8 293,006 + BT0262 0.69 -1.9 293,008 + BT0262 0.69 -0.6 293,053 - BT0262 0.76 -1.4 293,053 - BT0262 0.76 -0.2 293,062 + BT0262 0.78 +0.6 293,103 + BT0262 0.84 +2.0 293,126 - BT0262 0.88 -0.8 293,126 - BT0262 0.88 -0.2 293,128 - BT0262 0.88 -0.4 293,151 - +0.8
Or see this region's nucleotide sequence