Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0171 and BT0172 are separated by 114 nucleotides
BT0171: BT0171 - conserved hypothetical protein (NCBI ptt file), at 179,107 to 180,390
BT0171
BT0172: BT0172 - hypothetical protein (NCBI ptt file), at 180,505 to 180,900
BT0172
Position (kb) 180
181 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 179.550 kb on + strand, within BT0171 at 179.552 kb on + strand, within BT0171 at 179.572 kb on - strand, within BT0171 at 179.650 kb on + strand, within BT0171 at 179.710 kb on + strand, within BT0171 at 179.720 kb on + strand, within BT0171 at 179.740 kb on + strand, within BT0171 at 179.760 kb on + strand, within BT0171 at 179.763 kb on + strand, within BT0171 at 179.790 kb on + strand, within BT0171 at 179.864 kb on - strand, within BT0171 at 179.932 kb on + strand, within BT0171 at 179.932 kb on + strand, within BT0171 at 179.956 kb on + strand, within BT0171 at 179.961 kb on - strand, within BT0171 at 180.022 kb on + strand, within BT0171 at 180.022 kb on + strand, within BT0171 at 180.109 kb on - strand, within BT0171 at 180.154 kb on - strand, within BT0171 at 180.180 kb on - strand, within BT0171 at 180.250 kb on + strand, within BT0171 at 180.265 kb on - strand at 180.308 kb on - strand at 180.485 kb on - strand at 180.547 kb on - strand at 180.560 kb on - strand, within BT0172 at 180.623 kb on + strand, within BT0172 at 180.624 kb on - strand, within BT0172 at 180.643 kb on - strand, within BT0172 at 180.695 kb on - strand, within BT0172 at 180.698 kb on - strand, within BT0172 at 180.727 kb on + strand, within BT0172 at 180.727 kb on + strand, within BT0172 at 180.728 kb on - strand, within BT0172 at 180.731 kb on + strand, within BT0172 at 180.731 kb on + strand, within BT0172 at 180.731 kb on + strand, within BT0172 at 180.732 kb on - strand, within BT0172 at 180.732 kb on - strand, within BT0172 at 180.732 kb on - strand, within BT0172 at 180.749 kb on + strand, within BT0172 at 180.788 kb on - strand, within BT0172 at 180.838 kb on + strand, within BT0172 at 180.858 kb on + strand, within BT0172 at 180.859 kb on - strand, within BT0172 at 180.877 kb on - strand at 180.935 kb on + strand at 180.990 kb on + strand at 180.998 kb on + strand at 181.018 kb on - strand at 181.035 kb on - strand at 181.053 kb on + strand at 181.055 kb on + strand at 181.057 kb on + strand at 181.096 kb on - strand at 181.104 kb on + strand at 181.122 kb on + strand at 181.141 kb on - strand at 181.147 kb on - strand at 181.151 kb on + strand at 181.152 kb on - strand at 181.153 kb on + strand at 181.153 kb on + strand at 181.154 kb on - strand at 181.154 kb on - strand at 181.260 kb on - strand at 181.346 kb on + strand at 181.346 kb on + strand at 181.484 kb on - strand at 181.501 kb on + strand at 181.519 kb on + strand at 181.536 kb on - strand at 181.536 kb on - strand at 181.565 kb on + strand at 181.566 kb on - strand at 181.621 kb on - strand at 181.655 kb on + strand at 181.713 kb on + strand at 181.768 kb on - strand at 181.787 kb on + strand at 181.790 kb on - strand at 181.790 kb on - strand at 181.790 kb on - strand at 181.795 kb on + strand at 181.818 kb on + strand at 181.827 kb on + strand at 181.828 kb on - strand at 181.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 179,550 + BT0171 0.35 +1.3 179,552 + BT0171 0.35 -1.3 179,572 - BT0171 0.36 -0.4 179,650 + BT0171 0.42 -1.6 179,710 + BT0171 0.47 -0.5 179,720 + BT0171 0.48 -4.1 179,740 + BT0171 0.49 -1.7 179,760 + BT0171 0.51 +0.3 179,763 + BT0171 0.51 +0.2 179,790 + BT0171 0.53 -1.7 179,864 - BT0171 0.59 -0.1 179,932 + BT0171 0.64 -1.3 179,932 + BT0171 0.64 +0.1 179,956 + BT0171 0.66 -0.8 179,961 - BT0171 0.67 -0.4 180,022 + BT0171 0.71 +0.3 180,022 + BT0171 0.71 -3.4 180,109 - BT0171 0.78 -2.8 180,154 - BT0171 0.82 +2.9 180,180 - BT0171 0.84 -2.5 180,250 + BT0171 0.89 -0.5 180,265 - -0.6 180,308 - +0.0 180,485 - -0.5 180,547 - +0.7 180,560 - BT0172 0.14 -0.1 180,623 + BT0172 0.30 +2.0 180,624 - BT0172 0.30 +1.7 180,643 - BT0172 0.35 -1.5 180,695 - BT0172 0.48 -0.5 180,698 - BT0172 0.49 -1.0 180,727 + BT0172 0.56 +0.6 180,727 + BT0172 0.56 -1.3 180,728 - BT0172 0.56 -1.0 180,731 + BT0172 0.57 -0.0 180,731 + BT0172 0.57 -2.0 180,731 + BT0172 0.57 +0.3 180,732 - BT0172 0.57 -2.4 180,732 - BT0172 0.57 -2.2 180,732 - BT0172 0.57 -2.6 180,749 + BT0172 0.62 +0.4 180,788 - BT0172 0.71 -2.0 180,838 + BT0172 0.84 -3.8 180,858 + BT0172 0.89 +0.3 180,859 - BT0172 0.89 -0.6 180,877 - +1.3 180,935 + +0.1 180,990 + -2.4 180,998 + -0.8 181,018 - -0.1 181,035 - -1.5 181,053 + -0.2 181,055 + +0.1 181,057 + -0.3 181,096 - -2.3 181,104 + +0.0 181,122 + -0.7 181,141 - +0.4 181,147 - -0.7 181,151 + +1.6 181,152 - -1.9 181,153 + +1.5 181,153 + -0.2 181,154 - -1.8 181,154 - -2.9 181,260 - -1.1 181,346 + -0.7 181,346 + +0.8 181,484 - +1.2 181,501 + -1.9 181,519 + -2.4 181,536 - +0.5 181,536 - +1.3 181,565 + -0.4 181,566 - -2.1 181,621 - +1.0 181,655 + +0.6 181,713 + -1.2 181,768 - -2.7 181,787 + +1.2 181,790 - -0.8 181,790 - -3.2 181,790 - -1.1 181,795 + -0.1 181,818 + -2.5 181,827 + -1.0 181,828 - -0.1 181,895 - -1.1
Or see this region's nucleotide sequence