Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0015 and BT0016 are separated by 187 nucleotides BT0016 and BT0017 are separated by 112 nucleotides BT0017 and BT0018 are separated by 182 nucleotides BT0018 and BT0019 are separated by 11 nucleotides
BT0015: BT0015 - rteB, two-component system response regulator (NCBI ptt file), at 13,288 to 14,586
BT0015
BT0016: BT0016 - hypothetical protein (NCBI ptt file), at 14,774 to 15,412
BT0016
BT0017: BT0017 - hypothetical protein (NCBI ptt file), at 15,525 to 16,472
BT0017
BT0018: BT0018 - hypothetical protein (NCBI ptt file), at 16,655 to 17,371
BT0018
BT0019: BT0019 - hypothetical protein (NCBI ptt file), at 17,383 to 17,703
BT0019
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 14.638 kb on - strand at 14.638 kb on - strand at 14.696 kb on + strand at 14.723 kb on - strand at 14.740 kb on - strand at 14.791 kb on + strand at 15.109 kb on + strand, within BT0016 at 15.265 kb on - strand, within BT0016 at 15.306 kb on + strand, within BT0016 at 15.308 kb on + strand, within BT0016 at 15.309 kb on - strand, within BT0016 at 15.310 kb on + strand, within BT0016 at 15.340 kb on + strand, within BT0016 at 15.341 kb on - strand, within BT0016 at 15.381 kb on - strand at 15.448 kb on + strand at 15.449 kb on - strand at 15.449 kb on - strand at 15.621 kb on + strand, within BT0017 at 15.840 kb on - strand, within BT0017 at 16.075 kb on + strand, within BT0017 at 16.075 kb on + strand, within BT0017 at 16.089 kb on + strand, within BT0017 at 16.096 kb on - strand, within BT0017 at 16.096 kb on - strand, within BT0017 at 16.462 kb on + strand at 16.521 kb on - strand at 16.521 kb on - strand at 16.521 kb on - strand at 16.699 kb on - strand at 16.914 kb on + strand, within BT0018 at 16.971 kb on + strand, within BT0018 at 17.241 kb on + strand, within BT0018 at 17.287 kb on - strand, within BT0018 at 17.287 kb on - strand, within BT0018 at 17.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 14,638 - +0.1 14,638 - +0.3 14,696 + +1.3 14,723 - +0.6 14,740 - +2.9 14,791 + -0.3 15,109 + BT0016 0.52 -2.5 15,265 - BT0016 0.77 -2.3 15,306 + BT0016 0.83 -1.0 15,308 + BT0016 0.84 -0.8 15,309 - BT0016 0.84 +0.3 15,310 + BT0016 0.84 +1.3 15,340 + BT0016 0.89 -0.3 15,341 - BT0016 0.89 +0.7 15,381 - -1.8 15,448 + -1.3 15,449 - +0.3 15,449 - +1.2 15,621 + BT0017 0.10 -1.7 15,840 - BT0017 0.33 +0.2 16,075 + BT0017 0.58 -3.4 16,075 + BT0017 0.58 -4.6 16,089 + BT0017 0.59 -0.3 16,096 - BT0017 0.60 -1.7 16,096 - BT0017 0.60 +0.6 16,462 + -0.4 16,521 - +1.4 16,521 - +1.3 16,521 - +0.8 16,699 - -2.4 16,914 + BT0018 0.36 -3.3 16,971 + BT0018 0.44 +0.1 17,241 + BT0018 0.82 +2.3 17,287 - BT0018 0.88 +0.6 17,287 - BT0018 0.88 +1.3 17,398 - -1.7
Or see this region's nucleotide sequence