Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04735 and MMJJ_RS04740 are separated by 15 nucleotides MMJJ_RS04740 and MMJJ_RS04745 are separated by 77 nucleotides MMJJ_RS04745 and MMJJ_RS04750 are separated by 305 nucleotides
MMJJ_RS04735: MMJJ_RS04735 - fumarate hydratase, at 888,460 to 889,305
_RS04735
MMJJ_RS04740: MMJJ_RS04740 - histidinol phosphate phosphatase domain-containing protein, at 889,321 to 889,977
_RS04740
MMJJ_RS04745: MMJJ_RS04745 - cyclase family protein, at 890,055 to 890,624
_RS04745
MMJJ_RS04750: MMJJ_RS04750 - 5,10-methenyltetrahydromethanopterin hydrogenase, at 890,930 to 891,994
_RS04750
Position (kb) 890
891 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 889.322 kb on - strand at 889.455 kb on - strand, within MMJJ_RS04740 at 889.796 kb on - strand, within MMJJ_RS04740 at 889.797 kb on + strand, within MMJJ_RS04740 at 890.014 kb on - strand at 890.015 kb on + strand at 890.015 kb on + strand at 890.016 kb on - strand at 890.016 kb on - strand at 890.017 kb on + strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.020 kb on - strand at 890.021 kb on + strand at 890.023 kb on - strand at 890.026 kb on - strand at 890.026 kb on - strand at 890.031 kb on + strand at 890.034 kb on - strand at 890.034 kb on - strand at 890.042 kb on + strand at 890.071 kb on - strand at 890.072 kb on + strand at 890.104 kb on - strand at 890.104 kb on - strand at 890.155 kb on + strand, within MMJJ_RS04745 at 890.226 kb on + strand, within MMJJ_RS04745 at 890.268 kb on + strand, within MMJJ_RS04745 at 890.353 kb on + strand, within MMJJ_RS04745 at 890.363 kb on - strand, within MMJJ_RS04745 at 890.364 kb on + strand, within MMJJ_RS04745 at 890.364 kb on + strand, within MMJJ_RS04745 at 890.370 kb on - strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.371 kb on + strand, within MMJJ_RS04745 at 890.414 kb on + strand, within MMJJ_RS04745 at 890.421 kb on + strand, within MMJJ_RS04745 at 890.489 kb on + strand, within MMJJ_RS04745 at 890.507 kb on + strand, within MMJJ_RS04745 at 890.516 kb on - strand, within MMJJ_RS04745 at 890.517 kb on + strand, within MMJJ_RS04745 at 890.604 kb on + strand at 890.643 kb on + strand at 890.668 kb on + strand at 890.710 kb on + strand at 890.713 kb on + strand at 890.726 kb on - strand at 890.756 kb on + strand at 890.763 kb on + strand at 890.765 kb on - strand at 890.811 kb on + strand at 890.813 kb on + strand at 890.816 kb on - strand at 890.822 kb on - strand at 890.858 kb on - strand at 890.858 kb on - strand at 890.859 kb on + strand at 890.886 kb on - strand at 890.887 kb on + strand at 890.887 kb on + strand at 890.895 kb on - strand at 890.896 kb on + strand at 890.901 kb on + strand at 890.901 kb on + strand at 890.904 kb on + strand at 890.905 kb on - strand at 890.905 kb on - strand at 890.905 kb on - strand at 890.906 kb on + strand at 890.906 kb on + strand at 890.906 kb on + strand at 890.910 kb on - strand at 890.910 kb on - strand at 890.910 kb on - strand at 890.914 kb on - strand at 890.915 kb on + strand at 890.924 kb on - strand at 890.924 kb on - strand at 890.925 kb on + strand at 890.925 kb on + strand at 890.930 kb on + strand at 890.943 kb on - strand at 890.947 kb on + strand at 890.947 kb on + strand at 890.947 kb on + strand at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.087 kb on - strand, within MMJJ_RS04750 at 891.088 kb on + strand, within MMJJ_RS04750 at 891.100 kb on - strand, within MMJJ_RS04750 at 891.151 kb on + strand, within MMJJ_RS04750 at 891.153 kb on - strand, within MMJJ_RS04750 at 891.154 kb on + strand, within MMJJ_RS04750 at 891.182 kb on + strand, within MMJJ_RS04750 at 891.196 kb on + strand, within MMJJ_RS04750 at 891.241 kb on + strand, within MMJJ_RS04750 at 891.253 kb on - strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.295 kb on + strand, within MMJJ_RS04750 at 891.364 kb on + strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.367 kb on - strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.368 kb on + strand, within MMJJ_RS04750 at 891.402 kb on - strand, within MMJJ_RS04750 at 891.445 kb on + strand, within MMJJ_RS04750 at 891.458 kb on + strand, within MMJJ_RS04750 at 891.514 kb on + strand, within MMJJ_RS04750 at 891.527 kb on - strand, within MMJJ_RS04750 at 891.583 kb on + strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.611 kb on - strand, within MMJJ_RS04750 at 891.616 kb on - strand, within MMJJ_RS04750
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 889,322 - -0.7 889,455 - MMJJ_RS04740 0.20 -0.1 889,796 - MMJJ_RS04740 0.72 -3.3 889,797 + MMJJ_RS04740 0.72 -0.4 890,014 - +0.2 890,015 + -2.6 890,015 + -3.6 890,016 - +0.1 890,016 - +0.4 890,017 + -3.0 890,020 - +0.6 890,020 - -0.2 890,020 - -0.7 890,020 - -1.5 890,020 - +0.2 890,020 - -0.3 890,021 + -3.5 890,023 - -0.3 890,026 - -0.9 890,026 - +0.2 890,031 + -2.6 890,034 - -0.3 890,034 - +0.4 890,042 + +0.3 890,071 - +0.8 890,072 + +0.1 890,104 - +0.0 890,104 - +0.6 890,155 + MMJJ_RS04745 0.18 +0.4 890,226 + MMJJ_RS04745 0.30 +0.7 890,268 + MMJJ_RS04745 0.37 +0.1 890,353 + MMJJ_RS04745 0.52 -0.1 890,363 - MMJJ_RS04745 0.54 +0.6 890,364 + MMJJ_RS04745 0.54 +0.4 890,364 + MMJJ_RS04745 0.54 +0.0 890,370 - MMJJ_RS04745 0.55 -0.1 890,371 + MMJJ_RS04745 0.55 -3.0 890,371 + MMJJ_RS04745 0.55 -0.5 890,371 + MMJJ_RS04745 0.55 -0.1 890,414 + MMJJ_RS04745 0.63 +0.7 890,421 + MMJJ_RS04745 0.64 +0.2 890,489 + MMJJ_RS04745 0.76 +0.8 890,507 + MMJJ_RS04745 0.79 -0.6 890,516 - MMJJ_RS04745 0.81 -0.3 890,517 + MMJJ_RS04745 0.81 +0.8 890,604 + +0.3 890,643 + -0.2 890,668 + -0.1 890,710 + +0.2 890,713 + +0.2 890,726 - -0.7 890,756 + -0.1 890,763 + -0.1 890,765 - -0.4 890,811 + +0.2 890,813 + -1.0 890,816 - +0.4 890,822 - -1.8 890,858 - +1.1 890,858 - -0.1 890,859 + -0.9 890,886 - -0.4 890,887 + -1.5 890,887 + -2.1 890,895 - -1.8 890,896 + -0.8 890,901 + -3.4 890,901 + -1.8 890,904 + -1.9 890,905 - -2.9 890,905 - -1.9 890,905 - -1.5 890,906 + -3.4 890,906 + -2.7 890,906 + -0.3 890,910 - -2.6 890,910 - -1.9 890,910 - -3.2 890,914 - -1.2 890,915 + -1.7 890,924 - -1.5 890,924 - -3.0 890,925 + -1.6 890,925 + -0.8 890,930 + -2.0 890,943 - -2.2 890,947 + -0.6 890,947 + -1.8 890,947 + -1.6 891,087 - MMJJ_RS04750 0.15 -2.1 891,087 - MMJJ_RS04750 0.15 -2.8 891,087 - MMJJ_RS04750 0.15 +0.0 891,087 - MMJJ_RS04750 0.15 -2.7 891,088 + MMJJ_RS04750 0.15 -3.1 891,100 - MMJJ_RS04750 0.16 -3.3 891,151 + MMJJ_RS04750 0.21 -1.1 891,153 - MMJJ_RS04750 0.21 -2.9 891,154 + MMJJ_RS04750 0.21 -3.9 891,182 + MMJJ_RS04750 0.24 -2.0 891,196 + MMJJ_RS04750 0.25 -3.6 891,241 + MMJJ_RS04750 0.29 -2.6 891,253 - MMJJ_RS04750 0.30 -1.8 891,295 + MMJJ_RS04750 0.34 -2.5 891,295 + MMJJ_RS04750 0.34 -4.3 891,364 + MMJJ_RS04750 0.41 -0.7 891,367 - MMJJ_RS04750 0.41 -0.9 891,367 - MMJJ_RS04750 0.41 +0.2 891,368 + MMJJ_RS04750 0.41 -0.8 891,368 + MMJJ_RS04750 0.41 -1.0 891,402 - MMJJ_RS04750 0.44 -2.1 891,445 + MMJJ_RS04750 0.48 -1.7 891,458 + MMJJ_RS04750 0.50 -3.6 891,514 + MMJJ_RS04750 0.55 -2.4 891,527 - MMJJ_RS04750 0.56 -2.9 891,583 + MMJJ_RS04750 0.61 -2.1 891,611 - MMJJ_RS04750 0.64 +2.0 891,611 - MMJJ_RS04750 0.64 -2.8 891,616 - MMJJ_RS04750 0.64 -2.4
Or see this region's nucleotide sequence