Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01830

Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01820 and MMJJ_RS01825 are separated by 105 nucleotidesMMJJ_RS01825 and MMJJ_RS01830 are separated by 46 nucleotidesMMJJ_RS01830 and MMJJ_RS01835 are separated by 128 nucleotides MMJJ_RS01820: MMJJ_RS01820 - MJ1244 family protein, at 349,332 to 349,661 _RS01820 MMJJ_RS01825: MMJJ_RS01825 - DNA repair and recombination protein RadB, at 349,767 to 350,417 _RS01825 MMJJ_RS01830: MMJJ_RS01830 - tRNA(Ile)(2)-agmatinylcytidine synthase, at 350,464 to 351,738 _RS01830 MMJJ_RS01835: MMJJ_RS01835 - tRNA pseudouridine(13) synthase TruD, at 351,867 to 353,159 _RS01835 Position (kb) 350 351 352Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 349.480 kb on + strand, within MMJJ_RS01820at 349.677 kb on - strandat 349.677 kb on - strandat 349.677 kb on - strandat 349.696 kb on - strandat 349.696 kb on - strandat 349.696 kb on - strandat 349.712 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.716 kb on - strandat 349.718 kb on - strandat 349.719 kb on + strandat 349.751 kb on + strandat 349.781 kb on - strandat 349.809 kb on - strandat 349.863 kb on + strand, within MMJJ_RS01825at 349.863 kb on + strand, within MMJJ_RS01825at 349.921 kb on + strand, within MMJJ_RS01825at 349.954 kb on + strand, within MMJJ_RS01825at 349.954 kb on + strand, within MMJJ_RS01825at 349.976 kb on - strand, within MMJJ_RS01825at 350.055 kb on + strand, within MMJJ_RS01825at 350.093 kb on + strand, within MMJJ_RS01825at 350.120 kb on - strand, within MMJJ_RS01825at 350.120 kb on - strand, within MMJJ_RS01825at 350.121 kb on + strand, within MMJJ_RS01825at 350.121 kb on + strand, within MMJJ_RS01825at 350.211 kb on - strand, within MMJJ_RS01825at 350.334 kb on + strand, within MMJJ_RS01825at 350.373 kb on - strandat 350.417 kb on - strandat 350.418 kb on + strandat 350.444 kb on + strandat 350.449 kb on - strandat 350.461 kb on + strandat 350.461 kb on + strandat 350.465 kb on - strandat 350.692 kb on - strand, within MMJJ_RS01830at 350.803 kb on + strand, within MMJJ_RS01830at 350.928 kb on - strand, within MMJJ_RS01830at 351.034 kb on - strand, within MMJJ_RS01830at 351.151 kb on + strand, within MMJJ_RS01830at 351.248 kb on - strand, within MMJJ_RS01830at 351.253 kb on + strand, within MMJJ_RS01830at 351.400 kb on - strand, within MMJJ_RS01830at 351.569 kb on + strand, within MMJJ_RS01830at 351.675 kb on - strandat 351.686 kb on - strandat 351.708 kb on - strandat 351.734 kb on - strandat 351.750 kb on + strandat 351.808 kb on - strandat 351.808 kb on - strandat 351.809 kb on + strandat 351.809 kb on + strandat 351.812 kb on - strandat 351.813 kb on + strandat 351.813 kb on + strandat 351.813 kb on + strandat 351.813 kb on + strandat 351.813 kb on + strandat 351.817 kb on + strandat 351.835 kb on + strandat 351.837 kb on + strandat 351.839 kb on + strandat 351.839 kb on + strandat 351.839 kb on + strandat 351.839 kb on + strandat 351.863 kb on - strandat 351.881 kb on - strandat 351.916 kb on - strandat 351.916 kb on - strandat 352.016 kb on + strand, within MMJJ_RS01835at 352.016 kb on + strand, within MMJJ_RS01835at 352.016 kb on + strand, within MMJJ_RS01835at 352.079 kb on + strand, within MMJJ_RS01835at 352.099 kb on - strand, within MMJJ_RS01835at 352.202 kb on + strand, within MMJJ_RS01835at 352.213 kb on - strand, within MMJJ_RS01835at 352.213 kb on - strand, within MMJJ_RS01835at 352.214 kb on + strand, within MMJJ_RS01835at 352.214 kb on + strand, within MMJJ_RS01835at 352.214 kb on + strand, within MMJJ_RS01835at 352.277 kb on + strand, within MMJJ_RS01835at 352.280 kb on - strand, within MMJJ_RS01835at 352.331 kb on - strand, within MMJJ_RS01835at 352.344 kb on + strand, within MMJJ_RS01835at 352.344 kb on + strand, within MMJJ_RS01835at 352.432 kb on - strand, within MMJJ_RS01835at 352.472 kb on + strand, within MMJJ_RS01835at 352.482 kb on - strand, within MMJJ_RS01835at 352.482 kb on - strand, within MMJJ_RS01835at 352.482 kb on - strand, within MMJJ_RS01835at 352.482 kb on - strand, within MMJJ_RS01835at 352.483 kb on + strand, within MMJJ_RS01835at 352.486 kb on - strand, within MMJJ_RS01835at 352.486 kb on - strand, within MMJJ_RS01835at 352.487 kb on + strand, within MMJJ_RS01835at 352.489 kb on - strand, within MMJJ_RS01835at 352.489 kb on - strand, within MMJJ_RS01835at 352.490 kb on + strand, within MMJJ_RS01835at 352.495 kb on + strand, within MMJJ_RS01835at 352.539 kb on + strand, within MMJJ_RS01835at 352.558 kb on + strand, within MMJJ_RS01835at 352.558 kb on + strand, within MMJJ_RS01835at 352.558 kb on + strand, within MMJJ_RS01835at 352.584 kb on - strand, within MMJJ_RS01835at 352.590 kb on - strand, within MMJJ_RS01835at 352.590 kb on - strand, within MMJJ_RS01835at 352.590 kb on - strand, within MMJJ_RS01835at 352.591 kb on + strand, within MMJJ_RS01835at 352.591 kb on + strand, within MMJJ_RS01835at 352.606 kb on - strand, within MMJJ_RS01835at 352.607 kb on + strand, within MMJJ_RS01835at 352.686 kb on + strand, within MMJJ_RS01835at 352.722 kb on - strand, within MMJJ_RS01835at 352.723 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride
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349,480 + MMJJ_RS01820 0.45 -1.1
349,677 - -0.1
349,677 - -0.7
349,677 - -0.4
349,696 - -1.4
349,696 - -0.4
349,696 - -1.9
349,712 - -1.8
349,714 - -1.7
349,714 - -0.1
349,714 - -1.1
349,714 - -0.6
349,715 + -1.8
349,715 + -3.8
349,715 + -2.2
349,715 + -2.6
349,715 + -2.2
349,716 - +0.4
349,718 - +0.1
349,719 + +0.9
349,751 + -0.5
349,781 - +0.4
349,809 - +0.5
349,863 + MMJJ_RS01825 0.15 -0.2
349,863 + MMJJ_RS01825 0.15 -2.1
349,921 + MMJJ_RS01825 0.24 -0.1
349,954 + MMJJ_RS01825 0.29 +0.2
349,954 + MMJJ_RS01825 0.29 -0.4
349,976 - MMJJ_RS01825 0.32 +0.3
350,055 + MMJJ_RS01825 0.44 -0.7
350,093 + MMJJ_RS01825 0.50 -0.7
350,120 - MMJJ_RS01825 0.54 +0.3
350,120 - MMJJ_RS01825 0.54 -0.4
350,121 + MMJJ_RS01825 0.54 +1.0
350,121 + MMJJ_RS01825 0.54 -0.9
350,211 - MMJJ_RS01825 0.68 +0.6
350,334 + MMJJ_RS01825 0.87 +0.4
350,373 - +0.5
350,417 - +0.5
350,418 + +0.8
350,444 + -1.7
350,449 - -0.4
350,461 + -1.7
350,461 + -1.2
350,465 - -1.5
350,692 - MMJJ_RS01830 0.18 -1.9
350,803 + MMJJ_RS01830 0.27 -2.7
350,928 - MMJJ_RS01830 0.36 +0.9
351,034 - MMJJ_RS01830 0.45 -2.7
351,151 + MMJJ_RS01830 0.54 -2.1
351,248 - MMJJ_RS01830 0.61 -0.9
351,253 + MMJJ_RS01830 0.62 -0.1
351,400 - MMJJ_RS01830 0.73 -3.1
351,569 + MMJJ_RS01830 0.87 -2.1
351,675 - -2.6
351,686 - +1.7
351,708 - -1.1
351,734 - -0.6
351,750 + -2.4
351,808 - +0.3
351,808 - -0.1
351,809 + -2.5
351,809 + +0.5
351,812 - -1.4
351,813 + -0.7
351,813 + -1.1
351,813 + +0.0
351,813 + -0.9
351,813 + +0.8
351,817 + -0.2
351,835 + +0.9
351,837 + +0.4
351,839 + +0.1
351,839 + +0.7
351,839 + +0.0
351,839 + +0.1
351,863 - -0.0
351,881 - -0.4
351,916 - -0.3
351,916 - -0.1
352,016 + MMJJ_RS01835 0.12 -1.2
352,016 + MMJJ_RS01835 0.12 +1.0
352,016 + MMJJ_RS01835 0.12 +1.0
352,079 + MMJJ_RS01835 0.16 +1.1
352,099 - MMJJ_RS01835 0.18 -0.0
352,202 + MMJJ_RS01835 0.26 -0.7
352,213 - MMJJ_RS01835 0.27 -0.4
352,213 - MMJJ_RS01835 0.27 +0.3
352,214 + MMJJ_RS01835 0.27 -1.1
352,214 + MMJJ_RS01835 0.27 -0.5
352,214 + MMJJ_RS01835 0.27 -0.6
352,277 + MMJJ_RS01835 0.32 -0.2
352,280 - MMJJ_RS01835 0.32 -1.6
352,331 - MMJJ_RS01835 0.36 -0.0
352,344 + MMJJ_RS01835 0.37 -0.1
352,344 + MMJJ_RS01835 0.37 +0.5
352,432 - MMJJ_RS01835 0.44 -0.1
352,472 + MMJJ_RS01835 0.47 -0.1
352,482 - MMJJ_RS01835 0.48 -0.1
352,482 - MMJJ_RS01835 0.48 -0.7
352,482 - MMJJ_RS01835 0.48 -0.3
352,482 - MMJJ_RS01835 0.48 -0.1
352,483 + MMJJ_RS01835 0.48 -0.1
352,486 - MMJJ_RS01835 0.48 -0.4
352,486 - MMJJ_RS01835 0.48 +1.0
352,487 + MMJJ_RS01835 0.48 -0.1
352,489 - MMJJ_RS01835 0.48 -1.4
352,489 - MMJJ_RS01835 0.48 +0.0
352,490 + MMJJ_RS01835 0.48 -0.2
352,495 + MMJJ_RS01835 0.49 +0.6
352,539 + MMJJ_RS01835 0.52 -0.3
352,558 + MMJJ_RS01835 0.53 -0.5
352,558 + MMJJ_RS01835 0.53 +0.5
352,558 + MMJJ_RS01835 0.53 -0.9
352,584 - MMJJ_RS01835 0.55 -0.9
352,590 - MMJJ_RS01835 0.56 +0.8
352,590 - MMJJ_RS01835 0.56 -0.2
352,590 - MMJJ_RS01835 0.56 -0.4
352,591 + MMJJ_RS01835 0.56 +1.3
352,591 + MMJJ_RS01835 0.56 -0.2
352,606 - MMJJ_RS01835 0.57 -0.7
352,607 + MMJJ_RS01835 0.57 -0.7
352,686 + MMJJ_RS01835 0.63 -0.2
352,722 - MMJJ_RS01835 0.66 -0.6
352,723 + -1.1

Or see this region's nucleotide sequence