Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01465 and MMJJ_RS01470 are separated by 118 nucleotides MMJJ_RS01470 and MMJJ_RS01475 are separated by 163 nucleotides MMJJ_RS01475 and MMJJ_RS01480 are separated by 99 nucleotides MMJJ_RS01480 and MMJJ_RS01485 are separated by 162 nucleotides
MMJJ_RS01465: MMJJ_RS01465 - METTL5 family protein, at 286,896 to 287,537
_RS01465
MMJJ_RS01470: MMJJ_RS01470 - Hsp20/alpha crystallin family protein, at 287,656 to 288,120
_RS01470
MMJJ_RS01475: MMJJ_RS01475 - signal recognition particle-docking protein FtsY, at 288,284 to 289,435
_RS01475
MMJJ_RS01480: MMJJ_RS01480 - ECF transporter S component, at 289,535 to 290,101
_RS01480
MMJJ_RS01485: MMJJ_RS01485 - uracil-xanthine permease family protein, at 290,264 to 291,505
_RS01485
Position (kb) 288
289
290 Strain fitness (log2 ratio) -2
-1
0
1
2 at 287.359 kb on + strand, within MMJJ_RS01465 at 287.360 kb on - strand, within MMJJ_RS01465 at 287.361 kb on + strand, within MMJJ_RS01465 at 287.382 kb on - strand, within MMJJ_RS01465 at 287.408 kb on - strand, within MMJJ_RS01465 at 287.538 kb on - strand at 287.554 kb on + strand at 287.559 kb on - strand at 287.570 kb on - strand at 287.577 kb on + strand at 287.577 kb on + strand at 287.582 kb on - strand at 287.616 kb on - strand at 287.616 kb on - strand at 287.617 kb on + strand at 287.617 kb on + strand at 287.621 kb on + strand at 287.633 kb on - strand at 287.634 kb on + strand at 287.639 kb on - strand at 287.657 kb on - strand at 287.658 kb on + strand at 287.662 kb on - strand at 287.683 kb on + strand at 287.707 kb on - strand, within MMJJ_RS01470 at 287.775 kb on - strand, within MMJJ_RS01470 at 287.838 kb on - strand, within MMJJ_RS01470 at 287.894 kb on + strand, within MMJJ_RS01470 at 287.924 kb on + strand, within MMJJ_RS01470 at 287.968 kb on + strand, within MMJJ_RS01470 at 287.988 kb on - strand, within MMJJ_RS01470 at 287.989 kb on + strand, within MMJJ_RS01470 at 288.055 kb on + strand, within MMJJ_RS01470 at 288.201 kb on - strand at 288.202 kb on + strand at 288.202 kb on + strand at 288.202 kb on + strand at 288.206 kb on - strand at 288.210 kb on - strand at 288.216 kb on - strand at 288.217 kb on + strand at 288.219 kb on - strand at 288.219 kb on - strand at 288.219 kb on - strand at 288.220 kb on + strand at 288.220 kb on + strand at 288.221 kb on - strand at 288.221 kb on - strand at 288.221 kb on - strand at 288.222 kb on + strand at 288.222 kb on + strand at 288.222 kb on + strand at 288.226 kb on + strand at 288.238 kb on - strand at 288.238 kb on - strand at 288.238 kb on - strand at 288.238 kb on - strand at 288.238 kb on - strand at 288.240 kb on - strand at 288.241 kb on + strand at 288.747 kb on - strand, within MMJJ_RS01475 at 288.835 kb on + strand at 288.862 kb on + strand, within MMJJ_RS01475 at 289.079 kb on + strand, within MMJJ_RS01475 at 289.096 kb on - strand, within MMJJ_RS01475 at 289.250 kb on + strand, within MMJJ_RS01475 at 289.381 kb on + strand at 289.440 kb on - strand at 289.452 kb on - strand at 289.453 kb on + strand at 289.455 kb on + strand at 289.473 kb on - strand at 289.485 kb on - strand at 289.489 kb on - strand at 289.489 kb on - strand at 289.490 kb on + strand at 289.494 kb on + strand at 289.494 kb on + strand at 289.517 kb on - strand at 289.518 kb on + strand at 289.541 kb on + strand at 289.544 kb on + strand at 289.544 kb on + strand at 289.549 kb on + strand at 289.553 kb on - strand at 289.553 kb on - strand at 289.573 kb on - strand at 289.573 kb on - strand at 289.573 kb on - strand at 289.573 kb on - strand at 289.626 kb on - strand, within MMJJ_RS01480 at 289.648 kb on - strand, within MMJJ_RS01480 at 289.648 kb on - strand, within MMJJ_RS01480 at 289.649 kb on + strand, within MMJJ_RS01480 at 289.650 kb on - strand, within MMJJ_RS01480 at 289.651 kb on + strand, within MMJJ_RS01480 at 289.651 kb on + strand, within MMJJ_RS01480 at 289.654 kb on + strand, within MMJJ_RS01480 at 289.654 kb on + strand, within MMJJ_RS01480 at 289.662 kb on - strand, within MMJJ_RS01480 at 289.723 kb on - strand, within MMJJ_RS01480 at 289.730 kb on + strand, within MMJJ_RS01480 at 289.739 kb on - strand, within MMJJ_RS01480 at 289.740 kb on + strand, within MMJJ_RS01480 at 289.742 kb on + strand, within MMJJ_RS01480 at 289.758 kb on - strand, within MMJJ_RS01480 at 289.758 kb on - strand, within MMJJ_RS01480 at 289.758 kb on - strand, within MMJJ_RS01480 at 289.798 kb on - strand, within MMJJ_RS01480 at 289.799 kb on + strand, within MMJJ_RS01480 at 289.817 kb on + strand, within MMJJ_RS01480 at 289.830 kb on - strand, within MMJJ_RS01480 at 289.830 kb on - strand, within MMJJ_RS01480 at 289.830 kb on - strand, within MMJJ_RS01480 at 289.830 kb on - strand, within MMJJ_RS01480 at 289.830 kb on - strand, within MMJJ_RS01480 at 289.831 kb on + strand, within MMJJ_RS01480 at 289.831 kb on + strand, within MMJJ_RS01480 at 289.831 kb on + strand, within MMJJ_RS01480 at 289.854 kb on - strand, within MMJJ_RS01480 at 289.855 kb on + strand, within MMJJ_RS01480 at 289.855 kb on + strand, within MMJJ_RS01480 at 289.866 kb on - strand, within MMJJ_RS01480 at 289.866 kb on - strand, within MMJJ_RS01480 at 289.869 kb on - strand, within MMJJ_RS01480 at 289.870 kb on + strand, within MMJJ_RS01480 at 289.870 kb on + strand, within MMJJ_RS01480 at 289.872 kb on - strand, within MMJJ_RS01480 at 289.872 kb on - strand, within MMJJ_RS01480 at 289.873 kb on + strand, within MMJJ_RS01480 at 289.873 kb on + strand, within MMJJ_RS01480 at 289.873 kb on + strand, within MMJJ_RS01480 at 289.879 kb on + strand, within MMJJ_RS01480 at 289.936 kb on - strand, within MMJJ_RS01480 at 289.936 kb on - strand, within MMJJ_RS01480 at 289.936 kb on - strand, within MMJJ_RS01480 at 289.956 kb on - strand, within MMJJ_RS01480 at 289.957 kb on + strand, within MMJJ_RS01480 at 289.960 kb on - strand, within MMJJ_RS01480 at 289.975 kb on + strand, within MMJJ_RS01480 at 289.976 kb on - strand, within MMJJ_RS01480 at 289.976 kb on - strand, within MMJJ_RS01480 at 289.976 kb on - strand, within MMJJ_RS01480 at 289.977 kb on + strand, within MMJJ_RS01480 at 289.977 kb on + strand, within MMJJ_RS01480 at 290.033 kb on - strand, within MMJJ_RS01480 at 290.033 kb on - strand, within MMJJ_RS01480 at 290.033 kb on - strand, within MMJJ_RS01480 at 290.033 kb on - strand, within MMJJ_RS01480 at 290.034 kb on + strand, within MMJJ_RS01480 at 290.034 kb on + strand, within MMJJ_RS01480 at 290.037 kb on - strand, within MMJJ_RS01480 at 290.037 kb on - strand, within MMJJ_RS01480 at 290.038 kb on + strand, within MMJJ_RS01480 at 290.050 kb on + strand at 290.074 kb on + strand at 290.074 kb on + strand at 290.115 kb on - strand at 290.128 kb on - strand at 290.129 kb on + strand at 290.129 kb on + strand at 290.129 kb on + strand at 290.130 kb on - strand at 290.130 kb on - strand at 290.130 kb on - strand at 290.131 kb on + strand at 290.131 kb on + strand at 290.132 kb on - strand at 290.132 kb on - strand at 290.132 kb on - strand at 290.163 kb on - strand at 290.166 kb on - strand at 290.166 kb on - strand at 290.167 kb on + strand at 290.167 kb on + strand at 290.167 kb on + strand at 290.189 kb on - strand at 290.189 kb on - strand at 290.189 kb on - strand at 290.190 kb on + strand at 290.190 kb on + strand at 290.196 kb on - strand at 290.197 kb on + strand at 290.207 kb on - strand at 290.208 kb on + strand at 290.211 kb on - strand at 290.212 kb on + strand at 290.231 kb on - strand at 290.232 kb on + strand at 290.239 kb on + strand at 290.241 kb on - strand at 290.242 kb on + strand at 290.256 kb on - strand at 290.275 kb on + strand at 290.280 kb on - strand at 290.281 kb on + strand at 290.281 kb on + strand at 290.295 kb on - strand at 290.296 kb on + strand at 290.296 kb on + strand at 290.319 kb on - strand at 290.320 kb on + strand at 290.337 kb on - strand at 290.337 kb on - strand at 290.337 kb on - strand at 290.337 kb on - strand at 290.337 kb on - strand at 290.338 kb on + strand at 290.376 kb on - strand at 290.376 kb on - strand at 290.380 kb on + strand at 290.392 kb on + strand, within MMJJ_RS01485
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 287,359 + MMJJ_RS01465 0.72 -0.8 287,360 - MMJJ_RS01465 0.72 -0.8 287,361 + MMJJ_RS01465 0.72 -0.7 287,382 - MMJJ_RS01465 0.76 +0.4 287,408 - MMJJ_RS01465 0.80 -2.3 287,538 - +0.3 287,554 + -0.8 287,559 - +0.1 287,570 - -0.7 287,577 + +0.0 287,577 + +1.7 287,582 - -1.3 287,616 - -0.3 287,616 - +0.5 287,617 + +0.8 287,617 + +0.2 287,621 + +0.5 287,633 - +0.2 287,634 + +0.3 287,639 - +1.2 287,657 - +0.6 287,658 + -0.7 287,662 - +0.1 287,683 + -0.1 287,707 - MMJJ_RS01470 0.11 -0.9 287,775 - MMJJ_RS01470 0.26 +1.0 287,838 - MMJJ_RS01470 0.39 +0.4 287,894 + MMJJ_RS01470 0.51 -0.5 287,924 + MMJJ_RS01470 0.58 +0.1 287,968 + MMJJ_RS01470 0.67 -0.2 287,988 - MMJJ_RS01470 0.71 -0.3 287,989 + MMJJ_RS01470 0.72 +0.5 288,055 + MMJJ_RS01470 0.86 -0.5 288,201 - +0.7 288,202 + +0.7 288,202 + -0.7 288,202 + -1.7 288,206 - -1.2 288,210 - -0.9 288,216 - +0.2 288,217 + +0.8 288,219 - +0.6 288,219 - -0.3 288,219 - +0.3 288,220 + +0.9 288,220 + +0.0 288,221 - -0.2 288,221 - +0.2 288,221 - +0.1 288,222 + +0.3 288,222 + -1.5 288,222 + +0.3 288,226 + +0.6 288,238 - -0.1 288,238 - +0.7 288,238 - +1.1 288,238 - +1.3 288,238 - -1.3 288,240 - -0.8 288,241 + -0.2 288,747 - MMJJ_RS01475 0.40 -1.0 288,835 + +0.9 288,862 + MMJJ_RS01475 0.50 -1.7 289,079 + MMJJ_RS01475 0.69 -2.1 289,096 - MMJJ_RS01475 0.70 -2.3 289,250 + MMJJ_RS01475 0.84 -2.3 289,381 + +0.5 289,440 - +0.0 289,452 - +2.0 289,453 + +0.5 289,455 + +0.2 289,473 - -0.2 289,485 - -0.4 289,489 - -0.0 289,489 - +1.1 289,490 + -0.6 289,494 + -1.0 289,494 + +0.5 289,517 - +0.4 289,518 + +0.6 289,541 + -0.3 289,544 + +0.7 289,544 + -0.5 289,549 + +0.6 289,553 - -1.0 289,553 - -0.5 289,573 - -0.4 289,573 - +0.3 289,573 - -0.1 289,573 - +0.6 289,626 - MMJJ_RS01480 0.16 -0.1 289,648 - MMJJ_RS01480 0.20 +0.4 289,648 - MMJJ_RS01480 0.20 -0.0 289,649 + MMJJ_RS01480 0.20 -1.4 289,650 - MMJJ_RS01480 0.20 -0.9 289,651 + MMJJ_RS01480 0.20 -0.5 289,651 + MMJJ_RS01480 0.20 +0.7 289,654 + MMJJ_RS01480 0.21 -0.4 289,654 + MMJJ_RS01480 0.21 -0.9 289,662 - MMJJ_RS01480 0.22 -1.5 289,723 - MMJJ_RS01480 0.33 +0.3 289,730 + MMJJ_RS01480 0.34 +1.4 289,739 - MMJJ_RS01480 0.36 +0.5 289,740 + MMJJ_RS01480 0.36 -0.3 289,742 + MMJJ_RS01480 0.37 +0.0 289,758 - MMJJ_RS01480 0.39 +0.7 289,758 - MMJJ_RS01480 0.39 -0.7 289,758 - MMJJ_RS01480 0.39 +0.4 289,798 - MMJJ_RS01480 0.46 +0.0 289,799 + MMJJ_RS01480 0.47 +0.6 289,817 + MMJJ_RS01480 0.50 +0.1 289,830 - MMJJ_RS01480 0.52 +0.2 289,830 - MMJJ_RS01480 0.52 +0.3 289,830 - MMJJ_RS01480 0.52 -0.3 289,830 - MMJJ_RS01480 0.52 +0.2 289,830 - MMJJ_RS01480 0.52 +0.0 289,831 + MMJJ_RS01480 0.52 +0.5 289,831 + MMJJ_RS01480 0.52 -1.0 289,831 + MMJJ_RS01480 0.52 -0.6 289,854 - MMJJ_RS01480 0.56 -1.0 289,855 + MMJJ_RS01480 0.56 +0.3 289,855 + MMJJ_RS01480 0.56 +0.5 289,866 - MMJJ_RS01480 0.58 -0.1 289,866 - MMJJ_RS01480 0.58 +0.0 289,869 - MMJJ_RS01480 0.59 -1.8 289,870 + MMJJ_RS01480 0.59 +0.4 289,870 + MMJJ_RS01480 0.59 +0.3 289,872 - MMJJ_RS01480 0.59 -0.4 289,872 - MMJJ_RS01480 0.59 -0.9 289,873 + MMJJ_RS01480 0.60 -0.4 289,873 + MMJJ_RS01480 0.60 +1.0 289,873 + MMJJ_RS01480 0.60 -0.6 289,879 + MMJJ_RS01480 0.61 -0.2 289,936 - MMJJ_RS01480 0.71 -0.3 289,936 - MMJJ_RS01480 0.71 +1.1 289,936 - MMJJ_RS01480 0.71 -0.1 289,956 - MMJJ_RS01480 0.74 -1.0 289,957 + MMJJ_RS01480 0.74 -0.5 289,960 - MMJJ_RS01480 0.75 -0.0 289,975 + MMJJ_RS01480 0.78 -0.0 289,976 - MMJJ_RS01480 0.78 +0.8 289,976 - MMJJ_RS01480 0.78 -0.5 289,976 - MMJJ_RS01480 0.78 +0.6 289,977 + MMJJ_RS01480 0.78 -0.4 289,977 + MMJJ_RS01480 0.78 +0.6 290,033 - MMJJ_RS01480 0.88 +0.7 290,033 - MMJJ_RS01480 0.88 +0.3 290,033 - MMJJ_RS01480 0.88 +0.3 290,033 - MMJJ_RS01480 0.88 +0.2 290,034 + MMJJ_RS01480 0.88 +0.3 290,034 + MMJJ_RS01480 0.88 -0.5 290,037 - MMJJ_RS01480 0.89 -1.4 290,037 - MMJJ_RS01480 0.89 +0.3 290,038 + MMJJ_RS01480 0.89 +0.4 290,050 + -1.0 290,074 + -0.1 290,074 + -0.0 290,115 - -0.6 290,128 - -0.8 290,129 + -2.0 290,129 + -1.4 290,129 + -0.4 290,130 - -1.3 290,130 - +1.3 290,130 - +0.1 290,131 + -0.9 290,131 + +0.6 290,132 - +0.0 290,132 - -0.5 290,132 - +0.5 290,163 - -0.3 290,166 - +0.5 290,166 - +0.3 290,167 + -0.2 290,167 + -0.9 290,167 + +1.0 290,189 - -0.8 290,189 - -1.0 290,189 - -1.7 290,190 + -0.5 290,190 + +0.7 290,196 - -1.7 290,197 + -0.0 290,207 - -0.1 290,208 + -1.2 290,211 - +0.7 290,212 + -2.1 290,231 - +0.4 290,232 + -0.3 290,239 + -0.3 290,241 - +1.5 290,242 + +1.0 290,256 - +1.0 290,275 + -0.1 290,280 - -0.1 290,281 + -0.7 290,281 + +1.0 290,295 - -1.3 290,296 + +0.0 290,296 + -0.4 290,319 - -0.4 290,320 + -0.4 290,337 - +0.4 290,337 - -0.1 290,337 - +0.1 290,337 - +0.0 290,337 - -0.2 290,338 + +0.3 290,376 - -0.5 290,376 - -0.5 290,380 + +0.4 290,392 + MMJJ_RS01485 0.10 -0.2
Or see this region's nucleotide sequence