Experiment: PYE with Chloride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02558 and CCNA_02559 overlap by 4 nucleotides CCNA_02559 and CCNA_02560 are separated by 123 nucleotides CCNA_02560 and CCNA_02561 overlap by 4 nucleotides
CCNA_02558: CCNA_02558 - enantioselective peptide amidase, at 2,704,653 to 2,706,239
_02558
CCNA_02559: CCNA_02559 - NTF2 superfamily protein, at 2,706,236 to 2,706,730
_02559
CCNA_02560: CCNA_02560 - stalk cross-band protein StpC, at 2,706,854 to 2,707,492
_02560
CCNA_02561: CCNA_02561 - stalk cross-band protein StpB, at 2,707,489 to 2,708,913
_02561
Position (kb)
2706
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2705.856 kb on - strand, within CCNA_02558 at 2705.867 kb on - strand, within CCNA_02558 at 2705.874 kb on + strand at 2705.875 kb on - strand, within CCNA_02558 at 2705.875 kb on - strand, within CCNA_02558 at 2705.882 kb on - strand, within CCNA_02558 at 2705.902 kb on - strand, within CCNA_02558 at 2705.902 kb on - strand, within CCNA_02558 at 2705.902 kb on - strand, within CCNA_02558 at 2705.906 kb on + strand, within CCNA_02558 at 2705.909 kb on - strand, within CCNA_02558 at 2705.919 kb on + strand, within CCNA_02558 at 2705.937 kb on - strand, within CCNA_02558 at 2705.937 kb on - strand, within CCNA_02558 at 2705.937 kb on - strand, within CCNA_02558 at 2705.999 kb on + strand, within CCNA_02558 at 2705.999 kb on + strand, within CCNA_02558 at 2706.007 kb on + strand, within CCNA_02558 at 2706.007 kb on + strand, within CCNA_02558 at 2706.078 kb on + strand, within CCNA_02558 at 2706.103 kb on + strand at 2706.103 kb on + strand at 2706.111 kb on - strand at 2706.111 kb on - strand at 2706.143 kb on - strand at 2706.148 kb on - strand at 2706.178 kb on - strand at 2706.192 kb on + strand at 2706.215 kb on - strand at 2706.223 kb on - strand at 2706.296 kb on + strand, within CCNA_02559 at 2706.296 kb on + strand, within CCNA_02559 at 2706.297 kb on + strand, within CCNA_02559 at 2706.297 kb on + strand, within CCNA_02559 at 2706.297 kb on + strand, within CCNA_02559 at 2706.304 kb on - strand, within CCNA_02559 at 2706.305 kb on - strand, within CCNA_02559 at 2706.305 kb on - strand, within CCNA_02559 at 2706.329 kb on + strand, within CCNA_02559 at 2706.337 kb on - strand, within CCNA_02559 at 2706.337 kb on - strand, within CCNA_02559 at 2706.345 kb on + strand, within CCNA_02559 at 2706.365 kb on - strand, within CCNA_02559 at 2706.406 kb on - strand, within CCNA_02559 at 2706.406 kb on - strand, within CCNA_02559 at 2706.441 kb on + strand, within CCNA_02559 at 2706.484 kb on - strand, within CCNA_02559 at 2706.484 kb on - strand, within CCNA_02559 at 2706.511 kb on - strand, within CCNA_02559 at 2706.511 kb on - strand, within CCNA_02559 at 2706.522 kb on - strand, within CCNA_02559 at 2706.562 kb on - strand, within CCNA_02559 at 2706.623 kb on + strand, within CCNA_02559 at 2706.623 kb on + strand, within CCNA_02559 at 2706.631 kb on - strand, within CCNA_02559 at 2706.631 kb on - strand, within CCNA_02559 at 2706.696 kb on + strand at 2706.698 kb on + strand at 2706.698 kb on + strand at 2706.706 kb on - strand at 2706.709 kb on - strand at 2706.711 kb on - strand at 2706.845 kb on + strand at 2706.853 kb on - strand at 2706.890 kb on + strand at 2706.925 kb on - strand, within CCNA_02560 at 2706.930 kb on - strand, within CCNA_02560 at 2706.962 kb on + strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.970 kb on - strand, within CCNA_02560 at 2706.971 kb on - strand, within CCNA_02560 at 2707.041 kb on + strand, within CCNA_02560 at 2707.054 kb on - strand, within CCNA_02560 at 2707.070 kb on + strand, within CCNA_02560 at 2707.078 kb on - strand, within CCNA_02560 at 2707.078 kb on - strand, within CCNA_02560 at 2707.082 kb on + strand, within CCNA_02560 at 2707.103 kb on + strand, within CCNA_02560 at 2707.143 kb on + strand, within CCNA_02560 at 2707.151 kb on - strand, within CCNA_02560 at 2707.151 kb on - strand, within CCNA_02560 at 2707.171 kb on - strand, within CCNA_02560 at 2707.174 kb on + strand, within CCNA_02560 at 2707.174 kb on + strand, within CCNA_02560 at 2707.182 kb on - strand, within CCNA_02560 at 2707.184 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.189 kb on + strand, within CCNA_02560 at 2707.192 kb on - strand, within CCNA_02560 at 2707.192 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.197 kb on - strand, within CCNA_02560 at 2707.199 kb on + strand, within CCNA_02560 at 2707.199 kb on + strand, within CCNA_02560 at 2707.199 kb on + strand, within CCNA_02560 at 2707.240 kb on - strand, within CCNA_02560 at 2707.317 kb on + strand, within CCNA_02560 at 2707.318 kb on - strand, within CCNA_02560 at 2707.318 kb on - strand, within CCNA_02560 at 2707.318 kb on - strand, within CCNA_02560 at 2707.358 kb on + strand, within CCNA_02560 at 2707.361 kb on - strand, within CCNA_02560 at 2707.361 kb on - strand, within CCNA_02560 at 2707.361 kb on - strand, within CCNA_02560 at 2707.405 kb on + strand, within CCNA_02560 at 2707.407 kb on + strand, within CCNA_02560 at 2707.407 kb on + strand, within CCNA_02560 at 2707.408 kb on - strand, within CCNA_02560 at 2707.408 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.415 kb on - strand, within CCNA_02560 at 2707.428 kb on + strand, within CCNA_02560 at 2707.430 kb on + strand at 2707.430 kb on + strand at 2707.436 kb on - strand at 2707.456 kb on + strand at 2707.480 kb on - strand at 2707.480 kb on - strand at 2707.482 kb on + strand at 2707.512 kb on - strand at 2707.512 kb on - strand at 2707.563 kb on + strand at 2707.601 kb on + strand at 2707.601 kb on + strand at 2707.609 kb on - strand at 2707.633 kb on + strand, within CCNA_02561 at 2707.651 kb on - strand, within CCNA_02561 at 2707.699 kb on - strand, within CCNA_02561 at 2707.703 kb on + strand, within CCNA_02561 at 2707.703 kb on + strand, within CCNA_02561 at 2707.711 kb on - strand, within CCNA_02561 at 2707.724 kb on + strand, within CCNA_02561 at 2707.724 kb on + strand, within CCNA_02561 at 2707.725 kb on + strand, within CCNA_02561 at 2707.747 kb on + strand, within CCNA_02561 at 2707.755 kb on - strand, within CCNA_02561 at 2707.755 kb on - strand, within CCNA_02561 at 2707.765 kb on - strand, within CCNA_02561 at 2707.777 kb on - strand, within CCNA_02561 at 2707.847 kb on + strand, within CCNA_02561 at 2707.868 kb on + strand, within CCNA_02561 at 2707.868 kb on + strand, within CCNA_02561 at 2707.876 kb on - strand, within CCNA_02561 at 2707.880 kb on + strand, within CCNA_02561 at 2707.888 kb on - strand, within CCNA_02561 at 2707.888 kb on - strand, within CCNA_02561 at 2707.896 kb on + strand, within CCNA_02561 at 2707.946 kb on - strand, within CCNA_02561 at 2708.020 kb on - strand, within CCNA_02561 at 2708.026 kb on - strand, within CCNA_02561 at 2708.039 kb on - strand, within CCNA_02561 at 2708.216 kb on + strand, within CCNA_02561 at 2708.251 kb on - strand, within CCNA_02561 at 2708.256 kb on - strand, within CCNA_02561 at 2708.256 kb on - strand, within CCNA_02561 at 2708.259 kb on + strand, within CCNA_02561 at 2708.277 kb on - strand, within CCNA_02561 at 2708.364 kb on - strand, within CCNA_02561 at 2708.366 kb on + strand, within CCNA_02561 at 2708.397 kb on - strand, within CCNA_02561 at 2708.406 kb on - strand, within CCNA_02561 at 2708.406 kb on - strand, within CCNA_02561 at 2708.406 kb on - strand, within CCNA_02561 at 2708.435 kb on + strand, within CCNA_02561 at 2708.435 kb on + strand, within CCNA_02561
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Chloride 100 mM remove 2,705,856 - CCNA_02558 0.76 -1.7 2,705,867 - CCNA_02558 0.76 -0.9 2,705,874 + +1.4 2,705,875 - CCNA_02558 0.77 -0.4 2,705,875 - CCNA_02558 0.77 -1.9 2,705,882 - CCNA_02558 0.77 -1.6 2,705,902 - CCNA_02558 0.79 -1.7 2,705,902 - CCNA_02558 0.79 +3.4 2,705,902 - CCNA_02558 0.79 +0.4 2,705,906 + CCNA_02558 0.79 -1.0 2,705,909 - CCNA_02558 0.79 -0.5 2,705,919 + CCNA_02558 0.80 -0.7 2,705,937 - CCNA_02558 0.81 -1.2 2,705,937 - CCNA_02558 0.81 -1.5 2,705,937 - CCNA_02558 0.81 -0.9 2,705,999 + CCNA_02558 0.85 -0.2 2,705,999 + CCNA_02558 0.85 -0.8 2,706,007 + CCNA_02558 0.85 +1.3 2,706,007 + CCNA_02558 0.85 +1.7 2,706,078 + CCNA_02558 0.90 +1.2 2,706,103 + +2.7 2,706,103 + +1.2 2,706,111 - -0.2 2,706,111 - +0.4 2,706,143 - +0.6 2,706,148 - +0.6 2,706,178 - -1.9 2,706,192 + +0.1 2,706,215 - +0.3 2,706,223 - +2.2 2,706,296 + CCNA_02559 0.12 +0.9 2,706,296 + CCNA_02559 0.12 +1.4 2,706,297 + CCNA_02559 0.12 -0.4 2,706,297 + CCNA_02559 0.12 -1.0 2,706,297 + CCNA_02559 0.12 -0.3 2,706,304 - CCNA_02559 0.14 -1.7 2,706,305 - CCNA_02559 0.14 -0.7 2,706,305 - CCNA_02559 0.14 -0.6 2,706,329 + CCNA_02559 0.19 -0.1 2,706,337 - CCNA_02559 0.20 -0.4 2,706,337 - CCNA_02559 0.20 -1.0 2,706,345 + CCNA_02559 0.22 +0.1 2,706,365 - CCNA_02559 0.26 +0.8 2,706,406 - CCNA_02559 0.34 -0.9 2,706,406 - CCNA_02559 0.34 -0.6 2,706,441 + CCNA_02559 0.41 -0.5 2,706,484 - CCNA_02559 0.50 -1.5 2,706,484 - CCNA_02559 0.50 -0.7 2,706,511 - CCNA_02559 0.56 +0.4 2,706,511 - CCNA_02559 0.56 +0.8 2,706,522 - CCNA_02559 0.58 +0.1 2,706,562 - CCNA_02559 0.66 +1.5 2,706,623 + CCNA_02559 0.78 +2.4 2,706,623 + CCNA_02559 0.78 +0.1 2,706,631 - CCNA_02559 0.80 -0.6 2,706,631 - CCNA_02559 0.80 +0.4 2,706,696 + +0.0 2,706,698 + -1.7 2,706,698 + +0.4 2,706,706 - +0.5 2,706,709 - +0.8 2,706,711 - +1.8 2,706,845 + +0.6 2,706,853 - +1.9 2,706,890 + -0.3 2,706,925 - CCNA_02560 0.11 +1.1 2,706,930 - CCNA_02560 0.12 +1.4 2,706,962 + CCNA_02560 0.17 +1.4 2,706,970 - CCNA_02560 0.18 +1.8 2,706,970 - CCNA_02560 0.18 +1.4 2,706,970 - CCNA_02560 0.18 +4.0 2,706,970 - CCNA_02560 0.18 +1.7 2,706,970 - CCNA_02560 0.18 +1.0 2,706,971 - CCNA_02560 0.18 +2.2 2,707,041 + CCNA_02560 0.29 +0.4 2,707,054 - CCNA_02560 0.31 +2.0 2,707,070 + CCNA_02560 0.34 +0.2 2,707,078 - CCNA_02560 0.35 +2.0 2,707,078 - CCNA_02560 0.35 +1.2 2,707,082 + CCNA_02560 0.36 +0.4 2,707,103 + CCNA_02560 0.39 -0.4 2,707,143 + CCNA_02560 0.45 -0.4 2,707,151 - CCNA_02560 0.46 +1.3 2,707,151 - CCNA_02560 0.46 +1.5 2,707,171 - CCNA_02560 0.50 +2.2 2,707,174 + CCNA_02560 0.50 -1.0 2,707,174 + CCNA_02560 0.50 -0.6 2,707,182 - CCNA_02560 0.51 +1.5 2,707,184 + CCNA_02560 0.52 -0.4 2,707,189 + CCNA_02560 0.52 -0.6 2,707,189 + CCNA_02560 0.52 +0.1 2,707,189 + CCNA_02560 0.52 +0.6 2,707,189 + CCNA_02560 0.52 -1.7 2,707,192 - CCNA_02560 0.53 +1.5 2,707,192 - CCNA_02560 0.53 +1.9 2,707,197 - CCNA_02560 0.54 +1.7 2,707,197 - CCNA_02560 0.54 +1.7 2,707,197 - CCNA_02560 0.54 +0.4 2,707,197 - CCNA_02560 0.54 +1.3 2,707,197 - CCNA_02560 0.54 +3.0 2,707,199 + CCNA_02560 0.54 -1.6 2,707,199 + CCNA_02560 0.54 -1.7 2,707,199 + CCNA_02560 0.54 +0.4 2,707,240 - CCNA_02560 0.60 +1.9 2,707,317 + CCNA_02560 0.72 -0.0 2,707,318 - CCNA_02560 0.73 +1.5 2,707,318 - CCNA_02560 0.73 +1.3 2,707,318 - CCNA_02560 0.73 +1.5 2,707,358 + CCNA_02560 0.79 +0.8 2,707,361 - CCNA_02560 0.79 +1.4 2,707,361 - CCNA_02560 0.79 +1.6 2,707,361 - CCNA_02560 0.79 +1.1 2,707,405 + CCNA_02560 0.86 -0.6 2,707,407 + CCNA_02560 0.87 -0.2 2,707,407 + CCNA_02560 0.87 -1.2 2,707,408 - CCNA_02560 0.87 +1.0 2,707,408 - CCNA_02560 0.87 +2.3 2,707,415 - CCNA_02560 0.88 +3.4 2,707,415 - CCNA_02560 0.88 +0.7 2,707,415 - CCNA_02560 0.88 +1.0 2,707,415 - CCNA_02560 0.88 +2.6 2,707,415 - CCNA_02560 0.88 +1.3 2,707,428 + CCNA_02560 0.90 +1.8 2,707,430 + +0.4 2,707,430 + -2.9 2,707,436 - +1.0 2,707,456 + +4.0 2,707,480 - +1.4 2,707,480 - +2.0 2,707,482 + -0.6 2,707,512 - +1.3 2,707,512 - +1.1 2,707,563 + -0.7 2,707,601 + -0.9 2,707,601 + -1.7 2,707,609 - +2.3 2,707,633 + CCNA_02561 0.10 +0.4 2,707,651 - CCNA_02561 0.11 +0.9 2,707,699 - CCNA_02561 0.15 -0.9 2,707,703 + CCNA_02561 0.15 -0.4 2,707,703 + CCNA_02561 0.15 -0.7 2,707,711 - CCNA_02561 0.16 +0.6 2,707,724 + CCNA_02561 0.16 -0.6 2,707,724 + CCNA_02561 0.16 +2.4 2,707,725 + CCNA_02561 0.17 -0.2 2,707,747 + CCNA_02561 0.18 -0.2 2,707,755 - CCNA_02561 0.19 +1.7 2,707,755 - CCNA_02561 0.19 +0.6 2,707,765 - CCNA_02561 0.19 +1.4 2,707,777 - CCNA_02561 0.20 +0.1 2,707,847 + CCNA_02561 0.25 +1.4 2,707,868 + CCNA_02561 0.27 -0.9 2,707,868 + CCNA_02561 0.27 +0.4 2,707,876 - CCNA_02561 0.27 +0.9 2,707,880 + CCNA_02561 0.27 -0.4 2,707,888 - CCNA_02561 0.28 +0.1 2,707,888 - CCNA_02561 0.28 +1.2 2,707,896 + CCNA_02561 0.29 +1.8 2,707,946 - CCNA_02561 0.32 +0.3 2,708,020 - CCNA_02561 0.37 +1.2 2,708,026 - CCNA_02561 0.38 +1.6 2,708,039 - CCNA_02561 0.39 +2.0 2,708,216 + CCNA_02561 0.51 +0.8 2,708,251 - CCNA_02561 0.53 +1.6 2,708,256 - CCNA_02561 0.54 +1.1 2,708,256 - CCNA_02561 0.54 +1.1 2,708,259 + CCNA_02561 0.54 -1.0 2,708,277 - CCNA_02561 0.55 +0.8 2,708,364 - CCNA_02561 0.61 +0.8 2,708,366 + CCNA_02561 0.62 +1.4 2,708,397 - CCNA_02561 0.64 +1.8 2,708,406 - CCNA_02561 0.64 +1.7 2,708,406 - CCNA_02561 0.64 +2.8 2,708,406 - CCNA_02561 0.64 +1.7 2,708,435 + CCNA_02561 0.66 -1.2 2,708,435 + CCNA_02561 0.66 +0.5
Or see this region's nucleotide sequence