Experiment: PYE with Chloride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02027 and CCNA_02028 overlap by 4 nucleotides CCNA_02028 and CCNA_02029 overlap by 4 nucleotides CCNA_02029 and CCNA_02030 are separated by 2 nucleotides CCNA_02030 and CCNA_02031 are separated by 21 nucleotides
CCNA_02027: CCNA_02027 - NADH-quinone oxidoreductase chain E, at 2,173,251 to 2,173,937
_02027
CCNA_02028: CCNA_02028 - NTF2 superfamily protein, at 2,173,934 to 2,174,266
_02028
CCNA_02029: CCNA_02029 - NADH-quinone oxidoreductase chain D, at 2,174,263 to 2,175,513
_02029
CCNA_02030: CCNA_02030 - hypothetical protein, at 2,175,516 to 2,175,911
_02030
CCNA_02031: CCNA_02031 - NADH-quinone oxidoreductase chain C, at 2,175,933 to 2,176,526
_02031
Position (kb) 2174
2175
2176 Strain fitness (log2 ratio) -1
0
1
2
3 at 2173.948 kb on - strand at 2174.000 kb on - strand, within CCNA_02028 at 2174.159 kb on - strand, within CCNA_02028 at 2175.678 kb on - strand, within CCNA_02030 at 2175.782 kb on - strand, within CCNA_02030 at 2175.810 kb on - strand, within CCNA_02030 at 2175.914 kb on - strand at 2175.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Chloride 100 mM remove 2,173,948 - +3.1 2,174,000 - CCNA_02028 0.20 +2.3 2,174,159 - CCNA_02028 0.68 +3.3 2,175,678 - CCNA_02030 0.41 +2.8 2,175,782 - CCNA_02030 0.67 +3.5 2,175,810 - CCNA_02030 0.74 +2.8 2,175,914 - +2.8 2,175,941 - +2.5
Or see this region's nucleotide sequence