Experiment: PYE with Chloride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00255 and CCNA_00256 are separated by 18 nucleotides CCNA_00256 and CCNA_00257 are separated by 118 nucleotides CCNA_00257 and CCNA_00259 are separated by 342 nucleotides
CCNA_00255: CCNA_00255 - esterase, at 268,063 to 269,187
_00255
CCNA_00256: CCNA_00256 - pyridoxine kinase, at 269,206 to 270,057
_00256
CCNA_00257: CCNA_00257 - adenosylhomocysteinase, at 270,176 to 271,567
_00257
CCNA_00259: CCNA_00259 - hypothetical protein, at 271,910 to 272,620
_00259
Position (kb) 270
271
272 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 269.211 kb on - strand at 269.212 kb on - strand at 269.287 kb on + strand at 269.287 kb on + strand at 269.312 kb on + strand, within CCNA_00256 at 269.320 kb on - strand, within CCNA_00256 at 269.356 kb on + strand, within CCNA_00256 at 269.388 kb on - strand, within CCNA_00256 at 269.429 kb on + strand, within CCNA_00256 at 269.433 kb on - strand, within CCNA_00256 at 269.437 kb on - strand, within CCNA_00256 at 269.437 kb on - strand, within CCNA_00256 at 269.446 kb on + strand, within CCNA_00256 at 269.454 kb on - strand, within CCNA_00256 at 269.467 kb on + strand, within CCNA_00256 at 269.467 kb on + strand, within CCNA_00256 at 269.475 kb on - strand, within CCNA_00256 at 269.475 kb on - strand, within CCNA_00256 at 269.490 kb on - strand, within CCNA_00256 at 269.490 kb on - strand, within CCNA_00256 at 269.499 kb on + strand, within CCNA_00256 at 269.507 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.512 kb on - strand, within CCNA_00256 at 269.527 kb on - strand, within CCNA_00256 at 269.537 kb on - strand, within CCNA_00256 at 269.573 kb on + strand, within CCNA_00256 at 269.594 kb on + strand, within CCNA_00256 at 269.600 kb on - strand, within CCNA_00256 at 269.602 kb on + strand, within CCNA_00256 at 269.608 kb on + strand, within CCNA_00256 at 269.642 kb on - strand, within CCNA_00256 at 269.666 kb on + strand, within CCNA_00256 at 269.671 kb on + strand, within CCNA_00256 at 269.671 kb on + strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.679 kb on - strand, within CCNA_00256 at 269.701 kb on - strand, within CCNA_00256 at 269.717 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.725 kb on + strand, within CCNA_00256 at 269.733 kb on - strand at 269.733 kb on - strand, within CCNA_00256 at 269.733 kb on - strand, within CCNA_00256 at 269.773 kb on - strand, within CCNA_00256 at 269.773 kb on - strand, within CCNA_00256 at 269.801 kb on - strand, within CCNA_00256 at 269.819 kb on + strand, within CCNA_00256 at 269.824 kb on + strand, within CCNA_00256 at 269.827 kb on - strand, within CCNA_00256 at 269.864 kb on - strand, within CCNA_00256 at 269.872 kb on - strand, within CCNA_00256 at 269.873 kb on - strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.876 kb on + strand, within CCNA_00256 at 269.878 kb on + strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.884 kb on - strand, within CCNA_00256 at 269.925 kb on - strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.969 kb on + strand, within CCNA_00256 at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.977 kb on - strand at 269.979 kb on + strand at 269.979 kb on + strand at 269.987 kb on + strand at 269.987 kb on + strand at 269.992 kb on + strand at 269.992 kb on + strand at 269.995 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.000 kb on - strand at 270.010 kb on + strand at 270.064 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.084 kb on + strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.092 kb on - strand at 270.141 kb on + strand at 270.248 kb on - strand at 270.328 kb on - strand, within CCNA_00257 at 270.374 kb on - strand, within CCNA_00257 at 270.411 kb on - strand, within CCNA_00257 at 270.694 kb on - strand, within CCNA_00257 at 270.702 kb on - strand at 270.702 kb on - strand, within CCNA_00257 at 270.818 kb on - strand at 270.872 kb on - strand, within CCNA_00257 at 270.896 kb on - strand, within CCNA_00257 at 270.896 kb on - strand, within CCNA_00257 at 270.911 kb on - strand, within CCNA_00257 at 270.940 kb on - strand, within CCNA_00257 at 270.955 kb on - strand, within CCNA_00257 at 270.996 kb on - strand, within CCNA_00257 at 271.123 kb on - strand, within CCNA_00257 at 271.168 kb on - strand, within CCNA_00257 at 271.248 kb on - strand, within CCNA_00257 at 271.322 kb on - strand, within CCNA_00257 at 271.355 kb on - strand at 271.410 kb on - strand, within CCNA_00257 at 271.415 kb on - strand, within CCNA_00257 at 271.458 kb on - strand at 271.602 kb on + strand at 271.612 kb on + strand at 271.761 kb on + strand at 271.766 kb on + strand at 271.766 kb on + strand at 271.769 kb on - strand at 271.769 kb on - strand at 271.776 kb on + strand at 271.776 kb on + strand at 271.809 kb on + strand at 271.817 kb on - strand at 271.834 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.839 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.844 kb on + strand at 271.847 kb on - strand at 271.847 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.852 kb on - strand at 271.862 kb on - strand at 271.869 kb on - strand at 271.870 kb on + strand at 271.870 kb on + strand at 271.877 kb on - strand at 271.877 kb on - strand at 271.878 kb on - strand at 271.910 kb on - strand at 271.918 kb on + strand at 271.918 kb on - strand at 271.926 kb on - strand at 271.927 kb on - strand at 271.970 kb on - strand at 271.978 kb on - strand at 271.978 kb on - strand at 272.004 kb on - strand, within CCNA_00259 at 272.009 kb on - strand, within CCNA_00259 at 272.044 kb on + strand, within CCNA_00259 at 272.044 kb on + strand, within CCNA_00259 at 272.049 kb on + strand, within CCNA_00259 at 272.057 kb on - strand, within CCNA_00259 at 272.057 kb on - strand at 272.098 kb on - strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.110 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.117 kb on + strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.125 kb on - strand, within CCNA_00259 at 272.129 kb on + strand, within CCNA_00259 at 272.135 kb on + strand, within CCNA_00259 at 272.137 kb on - strand, within CCNA_00259 at 272.183 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.188 kb on + strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.191 kb on - strand, within CCNA_00259 at 272.207 kb on + strand, within CCNA_00259 at 272.215 kb on - strand, within CCNA_00259 at 272.255 kb on + strand, within CCNA_00259 at 272.255 kb on + strand, within CCNA_00259 at 272.268 kb on - strand, within CCNA_00259 at 272.272 kb on - strand, within CCNA_00259 at 272.296 kb on - strand, within CCNA_00259 at 272.300 kb on - strand, within CCNA_00259 at 272.357 kb on + strand, within CCNA_00259 at 272.409 kb on + strand, within CCNA_00259 at 272.448 kb on - strand, within CCNA_00259 at 272.459 kb on - strand, within CCNA_00259 at 272.492 kb on + strand, within CCNA_00259 at 272.492 kb on + strand, within CCNA_00259 at 272.511 kb on + strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.522 kb on + strand, within CCNA_00259 at 272.530 kb on - strand, within CCNA_00259 at 272.530 kb on - strand, within CCNA_00259 at 272.530 kb on - strand, within CCNA_00259 at 272.530 kb on - strand at 272.534 kb on + strand, within CCNA_00259 at 272.534 kb on + strand, within CCNA_00259 at 272.535 kb on + strand, within CCNA_00259 at 272.538 kb on - strand, within CCNA_00259 at 272.542 kb on - strand, within CCNA_00259 at 272.542 kb on - strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259 at 272.543 kb on + strand, within CCNA_00259 at 272.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Chloride 100 mM remove 269,211 - +0.7 269,212 - +0.6 269,287 + -0.3 269,287 + +1.0 269,312 + CCNA_00256 0.12 -0.5 269,320 - CCNA_00256 0.13 +0.0 269,356 + CCNA_00256 0.18 +1.4 269,388 - CCNA_00256 0.21 +0.9 269,429 + CCNA_00256 0.26 -0.8 269,433 - CCNA_00256 0.27 -0.3 269,437 - CCNA_00256 0.27 +0.3 269,437 - CCNA_00256 0.27 +1.4 269,446 + CCNA_00256 0.28 +1.0 269,454 - CCNA_00256 0.29 -0.6 269,467 + CCNA_00256 0.31 -1.1 269,467 + CCNA_00256 0.31 -0.6 269,475 - CCNA_00256 0.32 +1.0 269,475 - CCNA_00256 0.32 +0.6 269,490 - CCNA_00256 0.33 +1.3 269,490 - CCNA_00256 0.33 +0.5 269,499 + CCNA_00256 0.34 +2.0 269,507 - CCNA_00256 0.35 -0.2 269,512 - CCNA_00256 0.36 +1.8 269,512 - CCNA_00256 0.36 +1.5 269,512 - CCNA_00256 0.36 -0.9 269,527 - CCNA_00256 0.38 +0.0 269,537 - CCNA_00256 0.39 +0.6 269,573 + CCNA_00256 0.43 -0.3 269,594 + CCNA_00256 0.46 +0.4 269,600 - CCNA_00256 0.46 -0.8 269,602 + CCNA_00256 0.46 -1.3 269,608 + CCNA_00256 0.47 +0.2 269,642 - CCNA_00256 0.51 +3.0 269,666 + CCNA_00256 0.54 +0.6 269,671 + CCNA_00256 0.55 -0.7 269,671 + CCNA_00256 0.55 -1.6 269,679 - CCNA_00256 0.56 +3.3 269,679 - CCNA_00256 0.56 -1.5 269,679 - CCNA_00256 0.56 +0.4 269,679 - CCNA_00256 0.56 -0.2 269,701 - CCNA_00256 0.58 +0.8 269,717 + CCNA_00256 0.60 +0.0 269,725 + CCNA_00256 0.61 -0.3 269,725 + CCNA_00256 0.61 -1.0 269,725 + CCNA_00256 0.61 -0.2 269,725 + CCNA_00256 0.61 +0.8 269,733 - +1.0 269,733 - CCNA_00256 0.62 -0.6 269,733 - CCNA_00256 0.62 +1.1 269,773 - CCNA_00256 0.67 +0.4 269,773 - CCNA_00256 0.67 +1.2 269,801 - CCNA_00256 0.70 +0.9 269,819 + CCNA_00256 0.72 -0.1 269,824 + CCNA_00256 0.73 -0.3 269,827 - CCNA_00256 0.73 +0.5 269,864 - CCNA_00256 0.77 +1.0 269,872 - CCNA_00256 0.78 -1.0 269,873 - CCNA_00256 0.78 +0.2 269,876 + CCNA_00256 0.79 +0.5 269,876 + CCNA_00256 0.79 -0.7 269,876 + CCNA_00256 0.79 -0.6 269,878 + CCNA_00256 0.79 -0.5 269,884 - CCNA_00256 0.80 +0.6 269,884 - CCNA_00256 0.80 +0.1 269,884 - CCNA_00256 0.80 +0.2 269,925 - CCNA_00256 0.84 -0.7 269,969 + CCNA_00256 0.90 -1.6 269,969 + CCNA_00256 0.90 -2.1 269,969 + CCNA_00256 0.90 +0.0 269,969 + CCNA_00256 0.90 +0.9 269,969 + CCNA_00256 0.90 -2.6 269,969 + CCNA_00256 0.90 +2.0 269,977 - +0.1 269,977 - -0.1 269,977 - -0.8 269,977 - +0.3 269,977 - +1.7 269,977 - +1.1 269,977 - +1.5 269,979 + +0.7 269,979 + +0.4 269,987 + -0.9 269,987 + -0.8 269,992 + -0.6 269,992 + -0.6 269,995 - -1.9 270,000 - -0.4 270,000 - -0.5 270,000 - +1.5 270,000 - -0.3 270,000 - +0.9 270,000 - -1.1 270,000 - +0.5 270,010 + +0.2 270,064 + +0.4 270,084 + +2.0 270,084 + -1.2 270,084 + +0.3 270,084 + -2.2 270,084 + -0.9 270,084 + -1.9 270,092 - -0.0 270,092 - +0.1 270,092 - +1.7 270,092 - -0.7 270,092 - -0.8 270,092 - +0.7 270,092 - -1.6 270,092 - -0.2 270,092 - +0.4 270,092 - -1.3 270,092 - -1.1 270,092 - +0.5 270,141 + +0.0 270,248 - +3.8 270,328 - CCNA_00257 0.11 +3.6 270,374 - CCNA_00257 0.14 +3.4 270,411 - CCNA_00257 0.17 +2.1 270,694 - CCNA_00257 0.37 +3.2 270,702 - +4.5 270,702 - CCNA_00257 0.38 +2.6 270,818 - +3.4 270,872 - CCNA_00257 0.50 +2.6 270,896 - CCNA_00257 0.52 +2.6 270,896 - CCNA_00257 0.52 +2.2 270,911 - CCNA_00257 0.53 +3.2 270,940 - CCNA_00257 0.55 +1.2 270,955 - CCNA_00257 0.56 +4.3 270,996 - CCNA_00257 0.59 +2.8 271,123 - CCNA_00257 0.68 +2.4 271,168 - CCNA_00257 0.71 +3.5 271,248 - CCNA_00257 0.77 +2.9 271,322 - CCNA_00257 0.82 +3.7 271,355 - +4.3 271,410 - CCNA_00257 0.89 +2.6 271,415 - CCNA_00257 0.89 +3.8 271,458 - +2.0 271,602 + +1.0 271,612 + -0.9 271,761 + +0.6 271,766 + -0.7 271,766 + -0.5 271,769 - -0.5 271,769 - -2.3 271,776 + -0.4 271,776 + -1.2 271,809 + -3.0 271,817 - +0.4 271,834 + -1.5 271,839 + -1.5 271,839 + +0.9 271,839 + -1.3 271,844 + +0.0 271,844 + +2.0 271,844 + +0.2 271,844 + -0.3 271,844 + -1.7 271,844 + -1.7 271,844 + -0.9 271,844 + +0.0 271,844 + +0.0 271,847 - +0.3 271,847 - -0.0 271,852 - -0.3 271,852 - -0.3 271,852 - +0.7 271,852 - +1.0 271,852 - -1.2 271,852 - +1.5 271,852 - +0.5 271,852 - +0.2 271,852 - -0.1 271,852 - -1.7 271,852 - +0.3 271,852 - +0.4 271,852 - -0.3 271,852 - -0.3 271,862 - -1.6 271,869 - +0.2 271,870 + +0.4 271,870 + +1.0 271,877 - -0.9 271,877 - +0.2 271,878 - -1.4 271,910 - -0.8 271,918 + -0.1 271,918 - -0.2 271,926 - -0.1 271,927 - +0.5 271,970 - -0.2 271,978 - +0.8 271,978 - -1.5 272,004 - CCNA_00259 0.13 -0.9 272,009 - CCNA_00259 0.14 -0.8 272,044 + CCNA_00259 0.19 -1.3 272,044 + CCNA_00259 0.19 +2.0 272,049 + CCNA_00259 0.20 -1.2 272,057 - CCNA_00259 0.21 +1.0 272,057 - +1.0 272,098 - CCNA_00259 0.26 -0.5 272,110 + CCNA_00259 0.28 +3.0 272,110 + CCNA_00259 0.28 -1.5 272,117 + CCNA_00259 0.29 +1.0 272,117 + CCNA_00259 0.29 +2.0 272,117 + CCNA_00259 0.29 -0.3 272,125 - CCNA_00259 0.30 +0.2 272,125 - CCNA_00259 0.30 +0.8 272,125 - CCNA_00259 0.30 +0.3 272,125 - CCNA_00259 0.30 +0.1 272,129 + CCNA_00259 0.31 +0.3 272,135 + CCNA_00259 0.32 -3.1 272,137 - CCNA_00259 0.32 -1.2 272,183 + CCNA_00259 0.38 +0.4 272,188 + CCNA_00259 0.39 -1.8 272,188 + CCNA_00259 0.39 -0.3 272,188 + CCNA_00259 0.39 +0.0 272,191 - CCNA_00259 0.40 -1.7 272,191 - CCNA_00259 0.40 -2.0 272,191 - CCNA_00259 0.40 +0.1 272,207 + CCNA_00259 0.42 +0.4 272,215 - CCNA_00259 0.43 -1.8 272,255 + CCNA_00259 0.49 -1.2 272,255 + CCNA_00259 0.49 +1.0 272,268 - CCNA_00259 0.50 -1.3 272,272 - CCNA_00259 0.51 -2.2 272,296 - CCNA_00259 0.54 -1.0 272,300 - CCNA_00259 0.55 -2.1 272,357 + CCNA_00259 0.63 -0.7 272,409 + CCNA_00259 0.70 -1.7 272,448 - CCNA_00259 0.76 +0.4 272,459 - CCNA_00259 0.77 +1.0 272,492 + CCNA_00259 0.82 -0.8 272,492 + CCNA_00259 0.82 -1.9 272,511 + CCNA_00259 0.85 -0.6 272,522 + CCNA_00259 0.86 -0.7 272,522 + CCNA_00259 0.86 +0.3 272,530 - CCNA_00259 0.87 +2.0 272,530 - CCNA_00259 0.87 -1.0 272,530 - CCNA_00259 0.87 -1.8 272,530 - +0.4 272,534 + CCNA_00259 0.88 -1.2 272,534 + CCNA_00259 0.88 -1.7 272,535 + CCNA_00259 0.88 -0.6 272,538 - CCNA_00259 0.88 -1.0 272,542 - CCNA_00259 0.89 +0.0 272,542 - CCNA_00259 0.89 -2.1 272,543 + CCNA_00259 0.89 -1.3 272,543 + CCNA_00259 0.89 -1.2 272,543 + CCNA_00259 0.89 +0.0 272,554 - +0.0
Or see this region's nucleotide sequence