Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3005 and DvMF_3006 are separated by 108 nucleotides DvMF_3006 and thiE are separated by 5 nucleotides thiE and DvMF_3008 are separated by 227 nucleotides DvMF_3008 and DvMF_3009 are separated by 99 nucleotides
DvMF_3005: DvMF_3005 - transcriptional regulator, GntR family with aminotransferase domain (RefSeq), at 3,792,047 to 3,793,507
_3005
DvMF_3006: DvMF_3006 - hydroxyethylthiazole kinase (RefSeq), at 3,793,616 to 3,794,419
_3006
DvMF_3007: thiE - thiamine-phosphate pyrophosphorylase (RefSeq), at 3,794,425 to 3,795,066
thiE
DvMF_3008: DvMF_3008 - Dinitrogenase iron-molybdenum cofactor biosynthesis protein (RefSeq), at 3,795,294 to 3,795,629
_3008
DvMF_3009: DvMF_3009 - oxidoreductase FAD/NAD(P)-binding domain protein (RefSeq), at 3,795,729 to 3,796,493
_3009
Position (kb)
3794
3795
3796 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3794.042 kb on - strand, within DvMF_3006 at 3794.198 kb on + strand, within DvMF_3006 at 3794.653 kb on + strand, within thiE at 3794.942 kb on - strand, within thiE at 3794.985 kb on - strand, within thiE at 3795.080 kb on - strand at 3795.129 kb on - strand at 3795.200 kb on + strand at 3795.221 kb on + strand at 3795.221 kb on + strand at 3795.701 kb on + strand at 3795.712 kb on + strand at 3795.720 kb on - strand at 3795.834 kb on + strand, within DvMF_3009 at 3795.925 kb on + strand, within DvMF_3009
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 3,794,042 - DvMF_3006 0.53 -2.9 3,794,198 + DvMF_3006 0.72 -1.3 3,794,653 + thiE DvMF_3007 0.36 -1.8 3,794,942 - thiE DvMF_3007 0.81 -0.5 3,794,985 - thiE DvMF_3007 0.87 -2.7 3,795,080 - -0.4 3,795,129 - -1.0 3,795,200 + -0.8 3,795,221 + -3.4 3,795,221 + -0.1 3,795,701 + -4.1 3,795,712 + -3.3 3,795,720 - -0.2 3,795,834 + DvMF_3009 0.14 -1.5 3,795,925 + DvMF_3009 0.26 -0.1
Or see this region's nucleotide sequence