Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2973 and DvMF_2974 are separated by 87 nucleotides DvMF_2974 and DvMF_2975 are separated by 288 nucleotides DvMF_2975 and DvMF_2976 are separated by 1 nucleotides
DvMF_2973: DvMF_2973 - protein of unknown function DUF6 transmembrane (RefSeq), at 3,749,108 to 3,750,070
_2973
DvMF_2974: DvMF_2974 - transcriptional regulator, HxlR family (RefSeq), at 3,750,158 to 3,750,640
_2974
DvMF_2975: DvMF_2975 - nitroreductase (RefSeq), at 3,750,929 to 3,751,438
_2975
DvMF_2976: DvMF_2976 - 4-oxalocrotonate tautomerase (RefSeq), at 3,751,440 to 3,751,628
_2976
Position (kb)
3750
3751 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3749.208 kb on + strand, within DvMF_2973 at 3749.216 kb on - strand, within DvMF_2973 at 3749.221 kb on - strand, within DvMF_2973 at 3749.381 kb on + strand, within DvMF_2973 at 3749.692 kb on + strand, within DvMF_2973 at 3749.700 kb on - strand, within DvMF_2973 at 3749.817 kb on + strand, within DvMF_2973 at 3749.980 kb on - strand at 3749.989 kb on - strand at 3749.999 kb on - strand at 3750.179 kb on + strand at 3750.477 kb on - strand, within DvMF_2974 at 3750.521 kb on - strand, within DvMF_2974 at 3750.622 kb on - strand at 3750.993 kb on - strand, within DvMF_2975 at 3751.030 kb on + strand, within DvMF_2975 at 3751.058 kb on - strand, within DvMF_2975 at 3751.223 kb on + strand, within DvMF_2975 at 3751.264 kb on - strand, within DvMF_2975 at 3751.285 kb on - strand, within DvMF_2975
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 3,749,208 + DvMF_2973 0.10 +1.0 3,749,216 - DvMF_2973 0.11 -5.2 3,749,221 - DvMF_2973 0.12 -1.0 3,749,381 + DvMF_2973 0.28 -1.1 3,749,692 + DvMF_2973 0.61 -1.0 3,749,700 - DvMF_2973 0.61 -0.9 3,749,817 + DvMF_2973 0.74 +1.1 3,749,980 - -1.2 3,749,989 - +0.5 3,749,999 - -0.8 3,750,179 + -0.1 3,750,477 - DvMF_2974 0.66 -1.9 3,750,521 - DvMF_2974 0.75 -0.9 3,750,622 - -0.5 3,750,993 - DvMF_2975 0.13 -0.5 3,751,030 + DvMF_2975 0.20 -0.5 3,751,058 - DvMF_2975 0.25 +0.7 3,751,223 + DvMF_2975 0.58 -1.0 3,751,264 - DvMF_2975 0.66 +1.0 3,751,285 - DvMF_2975 0.70 -0.3
Or see this region's nucleotide sequence