Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_R0073 and ffs are separated by 25 nucleotides ffs and DvMF_2817 are separated by 248 nucleotides DvMF_2817 and DvMF_2818 are separated by 8 nucleotides DvMF_2818 and DvMF_2819 are separated by 326 nucleotides
DvMF_R0073: DvMF_R0073 - tRNA-Ser (RefSeq), at 3,562,548 to 3,562,639
_R0073
DvMF_R0074: ffs - SRP RNA; RNA component of signal recognitionparti cle (RefSeq), at 3,562,665 to 3,562,753
ffs
DvMF_2817: DvMF_2817 - hypothetical protein (RefSeq), at 3,563,002 to 3,563,328
_2817
DvMF_2818: DvMF_2818 - methylated-DNA/protein-cysteine methyltransferase (RefSeq), at 3,563,337 to 3,563,981
_2818
DvMF_2819: DvMF_2819 - conserved hypothetical radical SAM protein (RefSeq), at 3,564,308 to 3,565,426
_2819
Position (kb)
3563
3564 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3562.448 kb on + strand at 3562.448 kb on + strand at 3562.466 kb on - strand at 3562.935 kb on + strand at 3563.007 kb on + strand at 3563.015 kb on - strand at 3563.015 kb on - strand at 3563.023 kb on + strand at 3563.024 kb on + strand at 3563.238 kb on + strand, within DvMF_2817 at 3563.253 kb on + strand, within DvMF_2817 at 3563.253 kb on + strand, within DvMF_2817 at 3563.261 kb on - strand, within DvMF_2817 at 3563.261 kb on - strand, within DvMF_2817 at 3563.261 kb on - strand, within DvMF_2817 at 3563.323 kb on - strand at 3563.330 kb on + strand at 3563.338 kb on - strand at 3563.501 kb on + strand, within DvMF_2818 at 3563.806 kb on - strand, within DvMF_2818 at 3563.852 kb on - strand, within DvMF_2818 at 3564.249 kb on - strand at 3564.267 kb on + strand at 3564.267 kb on + strand at 3564.275 kb on - strand at 3564.275 kb on - strand at 3564.296 kb on - strand at 3564.348 kb on + strand at 3564.383 kb on + strand at 3564.386 kb on - strand at 3564.484 kb on - strand, within DvMF_2819 at 3564.553 kb on - strand, within DvMF_2819 at 3564.553 kb on - strand, within DvMF_2819 at 3564.707 kb on + strand, within DvMF_2819 at 3564.789 kb on - strand, within DvMF_2819 at 3564.805 kb on + strand, within DvMF_2819 at 3564.853 kb on - strand, within DvMF_2819 at 3564.869 kb on - strand, within DvMF_2819
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 3,562,448 + -1.3 3,562,448 + -0.1 3,562,466 - -0.5 3,562,935 + -0.7 3,563,007 + -0.9 3,563,015 - -1.4 3,563,015 - +0.4 3,563,023 + +0.0 3,563,024 + -0.2 3,563,238 + DvMF_2817 0.72 +0.2 3,563,253 + DvMF_2817 0.77 -2.3 3,563,253 + DvMF_2817 0.77 -0.6 3,563,261 - DvMF_2817 0.79 -0.6 3,563,261 - DvMF_2817 0.79 -4.1 3,563,261 - DvMF_2817 0.79 -1.8 3,563,323 - -2.4 3,563,330 + -3.9 3,563,338 - -0.6 3,563,501 + DvMF_2818 0.25 +0.4 3,563,806 - DvMF_2818 0.73 -0.7 3,563,852 - DvMF_2818 0.80 -0.5 3,564,249 - -1.2 3,564,267 + +0.2 3,564,267 + -1.0 3,564,275 - -1.4 3,564,275 - -2.4 3,564,296 - -0.7 3,564,348 + -3.2 3,564,383 + -1.1 3,564,386 - -1.0 3,564,484 - DvMF_2819 0.16 -0.7 3,564,553 - DvMF_2819 0.22 -3.9 3,564,553 - DvMF_2819 0.22 -1.0 3,564,707 + DvMF_2819 0.36 -5.0 3,564,789 - DvMF_2819 0.43 -0.4 3,564,805 + DvMF_2819 0.44 +1.0 3,564,853 - DvMF_2819 0.49 -0.5 3,564,869 - DvMF_2819 0.50 -0.8
Or see this region's nucleotide sequence