Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2728 and DvMF_2729 are separated by 79 nucleotides DvMF_2729 and DvMF_2730 are separated by 91 nucleotides DvMF_2730 and DvMF_2731 are separated by 179 nucleotides
DvMF_2728: DvMF_2728 - magnesium chelatase ChlI subunit (RefSeq), at 3,452,644 to 3,453,618
_2728
DvMF_2729: DvMF_2729 - hypothetical protein (RefSeq), at 3,453,698 to 3,454,342
_2729
DvMF_2730: DvMF_2730 - hypothetical protein (RefSeq), at 3,454,434 to 3,454,952
_2730
DvMF_2731: DvMF_2731 - PAS/PAC sensor hybrid histidine kinase (RefSeq), at 3,455,132 to 3,458,155
_2731
Position (kb)
3453
3454
3455 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3452.814 kb on + strand, within DvMF_2728 at 3452.814 kb on + strand, within DvMF_2728 at 3453.289 kb on - strand, within DvMF_2728 at 3453.388 kb on - strand, within DvMF_2728 at 3453.558 kb on - strand at 3453.588 kb on - strand at 3453.625 kb on + strand at 3453.633 kb on - strand at 3453.759 kb on - strand at 3453.807 kb on - strand, within DvMF_2729 at 3454.031 kb on + strand, within DvMF_2729 at 3454.044 kb on - strand, within DvMF_2729 at 3454.062 kb on + strand, within DvMF_2729 at 3454.062 kb on + strand, within DvMF_2729 at 3454.118 kb on + strand, within DvMF_2729 at 3454.126 kb on - strand, within DvMF_2729 at 3454.300 kb on + strand at 3454.319 kb on + strand at 3454.340 kb on - strand at 3454.813 kb on + strand, within DvMF_2730 at 3454.996 kb on - strand at 3455.030 kb on - strand at 3455.180 kb on - strand at 3455.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 3,452,814 + DvMF_2728 0.17 -1.3 3,452,814 + DvMF_2728 0.17 -3.1 3,453,289 - DvMF_2728 0.66 -1.6 3,453,388 - DvMF_2728 0.76 -1.2 3,453,558 - -0.0 3,453,588 - -0.5 3,453,625 + -0.9 3,453,633 - -2.1 3,453,759 - -1.7 3,453,807 - DvMF_2729 0.17 -3.5 3,454,031 + DvMF_2729 0.52 -3.0 3,454,044 - DvMF_2729 0.54 -1.0 3,454,062 + DvMF_2729 0.56 -3.0 3,454,062 + DvMF_2729 0.56 -0.6 3,454,118 + DvMF_2729 0.65 -1.7 3,454,126 - DvMF_2729 0.66 -0.6 3,454,300 + -0.9 3,454,319 + -0.8 3,454,340 - +0.5 3,454,813 + DvMF_2730 0.73 -0.3 3,454,996 - +0.1 3,455,030 - -0.5 3,455,180 - -0.1 3,455,196 + -2.9
Or see this region's nucleotide sequence