Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2431 and DvMF_2432 are separated by 19 nucleotides DvMF_2432 and DvMF_2433 are separated by 9 nucleotides DvMF_2433 and DvMF_2434 overlap by 22 nucleotides DvMF_2434 and DvMF_2435 are separated by 47 nucleotides
DvMF_2431: DvMF_2431 - hypothetical protein (RefSeq), at 3,067,197 to 3,067,970
_2431
DvMF_2432: DvMF_2432 - type III secretion apparatus lipoprotein, YscJ/HrcJ family (RefSeq), at 3,067,990 to 3,068,823
_2432
DvMF_2433: DvMF_2433 - hypothetical protein (RefSeq), at 3,068,833 to 3,069,570
_2433
DvMF_2434: DvMF_2434 - type III secretion system protein (RefSeq), at 3,069,549 to 3,070,166
_2434
DvMF_2435: DvMF_2435 - type III secretion system ATPase (RefSeq), at 3,070,214 to 3,071,530
_2435
Position (kb)
3068
3069
3070 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3067.862 kb on - strand, within DvMF_2431 at 3067.952 kb on + strand at 3068.118 kb on + strand, within DvMF_2432 at 3068.118 kb on + strand, within DvMF_2432 at 3068.126 kb on - strand, within DvMF_2432 at 3068.825 kb on - strand at 3068.825 kb on - strand at 3068.943 kb on - strand, within DvMF_2433 at 3069.371 kb on + strand, within DvMF_2433 at 3069.627 kb on + strand, within DvMF_2434 at 3069.633 kb on - strand, within DvMF_2434 at 3069.704 kb on + strand, within DvMF_2434 at 3070.269 kb on + strand at 3070.292 kb on + strand at 3070.332 kb on + strand at 3070.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 3,067,862 - DvMF_2431 0.86 -0.6 3,067,952 + -2.5 3,068,118 + DvMF_2432 0.15 -0.1 3,068,118 + DvMF_2432 0.15 -0.7 3,068,126 - DvMF_2432 0.16 -1.4 3,068,825 - +0.2 3,068,825 - -0.9 3,068,943 - DvMF_2433 0.15 -3.5 3,069,371 + DvMF_2433 0.73 +0.9 3,069,627 + DvMF_2434 0.13 -1.5 3,069,633 - DvMF_2434 0.14 +0.7 3,069,704 + DvMF_2434 0.25 -0.4 3,070,269 + -1.4 3,070,292 + -0.1 3,070,332 + -0.3 3,070,340 - -0.0
Or see this region's nucleotide sequence