Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2313 and DvMF_2314 are separated by 50 nucleotides DvMF_2314 and DvMF_2315 overlap by 4 nucleotides DvMF_2315 and DvMF_2316 overlap by 29 nucleotides DvMF_2316 and DvMF_2317 are separated by 42 nucleotides
DvMF_2313: DvMF_2313 - DNA-directed DNA polymerase (RefSeq), at 2,911,502 to 2,912,947
_2313
DvMF_2314: DvMF_2314 - aminotransferase class IV (RefSeq), at 2,912,998 to 2,913,771
_2314
DvMF_2315: DvMF_2315 - Chorismate binding-like protein (RefSeq), at 2,913,768 to 2,915,411
_2315
DvMF_2316: DvMF_2316 - glutamine amidotransferase class-I (RefSeq), at 2,915,383 to 2,916,225
_2316
DvMF_2317: DvMF_2317 - protein of unknown function DUF152 (RefSeq), at 2,916,268 to 2,917,101
_2317
Position (kb)
2913
2914
2915
2916 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2912.980 kb on + strand at 2913.192 kb on - strand, within DvMF_2314 at 2913.194 kb on + strand, within DvMF_2314 at 2913.796 kb on + strand at 2913.796 kb on + strand at 2913.845 kb on - strand at 2913.899 kb on - strand at 2914.103 kb on + strand, within DvMF_2315 at 2914.169 kb on - strand, within DvMF_2315 at 2914.228 kb on + strand, within DvMF_2315 at 2914.236 kb on - strand, within DvMF_2315 at 2914.470 kb on - strand, within DvMF_2315 at 2914.478 kb on + strand, within DvMF_2315 at 2914.514 kb on + strand, within DvMF_2315 at 2914.566 kb on + strand, within DvMF_2315 at 2914.757 kb on + strand, within DvMF_2315 at 2914.757 kb on + strand, within DvMF_2315 at 2914.843 kb on - strand, within DvMF_2315 at 2915.109 kb on - strand, within DvMF_2315 at 2915.174 kb on + strand, within DvMF_2315 at 2915.261 kb on + strand at 2915.360 kb on - strand at 2915.364 kb on + strand at 2915.585 kb on - strand, within DvMF_2316 at 2915.669 kb on + strand, within DvMF_2316 at 2915.693 kb on - strand, within DvMF_2316 at 2915.699 kb on - strand, within DvMF_2316 at 2916.045 kb on + strand, within DvMF_2316 at 2916.076 kb on - strand, within DvMF_2316 at 2916.237 kb on - strand at 2916.255 kb on + strand at 2916.360 kb on + strand, within DvMF_2317
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 2,912,980 + -1.8 2,913,192 - DvMF_2314 0.25 -0.4 2,913,194 + DvMF_2314 0.25 +0.4 2,913,796 + -1.2 2,913,796 + -1.6 2,913,845 - -0.5 2,913,899 - -0.1 2,914,103 + DvMF_2315 0.20 -2.1 2,914,169 - DvMF_2315 0.24 -1.0 2,914,228 + DvMF_2315 0.28 -1.2 2,914,236 - DvMF_2315 0.28 -4.6 2,914,470 - DvMF_2315 0.43 -0.3 2,914,478 + DvMF_2315 0.43 -0.4 2,914,514 + DvMF_2315 0.45 -3.7 2,914,566 + DvMF_2315 0.49 -0.1 2,914,757 + DvMF_2315 0.60 -4.3 2,914,757 + DvMF_2315 0.60 -0.4 2,914,843 - DvMF_2315 0.65 -4.5 2,915,109 - DvMF_2315 0.82 -1.3 2,915,174 + DvMF_2315 0.86 -0.8 2,915,261 + -0.3 2,915,360 - -0.0 2,915,364 + -1.3 2,915,585 - DvMF_2316 0.24 -0.7 2,915,669 + DvMF_2316 0.34 -3.3 2,915,693 - DvMF_2316 0.37 +0.2 2,915,699 - DvMF_2316 0.37 -1.0 2,916,045 + DvMF_2316 0.79 -0.6 2,916,076 - DvMF_2316 0.82 -0.7 2,916,237 - -0.9 2,916,255 + -0.0 2,916,360 + DvMF_2317 0.11 -1.3
Or see this region's nucleotide sequence