Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0347 and DvMF_0348 are separated by 199 nucleotides DvMF_0348 and secG are separated by 38 nucleotides secG and DvMF_R0017 are separated by 36 nucleotides DvMF_R0017 and DvMF_0350 are separated by 200 nucleotides
DvMF_0347: DvMF_0347 - ribosomal-protein-alanine acetyltransferase (RefSeq), at 405,948 to 406,526
_0347
DvMF_0348: DvMF_0348 - Triose-phosphate isomerase (RefSeq), at 406,726 to 407,481
_0348
DvMF_0349: secG - preprotein translocase subunit SecG (RefSeq), at 407,520 to 407,864
secG
DvMF_R0017: DvMF_R0017 - tRNA-Leu (RefSeq), at 407,901 to 407,987
_R0017
DvMF_0350: DvMF_0350 - extracellular solute-binding protein family 3 (RefSeq), at 408,188 to 409,153
_0350
Position (kb) 407
408 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 406.529 kb on - strand at 406.529 kb on - strand at 406.646 kb on - strand at 406.696 kb on + strand at 406.714 kb on + strand at 407.179 kb on + strand, within DvMF_0348 at 407.238 kb on + strand, within DvMF_0348 at 408.258 kb on + strand at 408.555 kb on - strand, within DvMF_0350 at 408.670 kb on + strand, within DvMF_0350 at 408.795 kb on + strand, within DvMF_0350 at 408.837 kb on - strand, within DvMF_0350
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 406,529 - -0.6 406,529 - -0.3 406,646 - +2.1 406,696 + -0.5 406,714 + -3.4 407,179 + DvMF_0348 0.60 -2.4 407,238 + DvMF_0348 0.68 -1.7 408,258 + -1.1 408,555 - DvMF_0350 0.38 -1.1 408,670 + DvMF_0350 0.50 +0.7 408,795 + DvMF_0350 0.63 -0.5 408,837 - DvMF_0350 0.67 -0.5
Or see this region's nucleotide sequence