Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0317 and DvMF_0318 overlap by 4 nucleotides DvMF_0318 and DvMF_0319 are separated by 209 nucleotides DvMF_0319 and DvMF_0320 are separated by 10 nucleotides
DvMF_0317: DvMF_0317 - putative signal transduction protein with CBS domains (RefSeq), at 367,705 to 369,897
_0317
DvMF_0318: DvMF_0318 - Exonuclease RNase T and DNA polymerase III (RefSeq), at 369,894 to 370,697
_0318
DvMF_0319: DvMF_0319 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 370,907 to 371,416
_0319
DvMF_0320: DvMF_0320 - Formate dehydrogenase (RefSeq), at 371,427 to 373,529
_0320
Position (kb) 369
370
371 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 369.065 kb on + strand, within DvMF_0317 at 369.182 kb on + strand, within DvMF_0317 at 369.258 kb on - strand, within DvMF_0317 at 369.258 kb on - strand, within DvMF_0317 at 369.278 kb on - strand, within DvMF_0317 at 369.282 kb on - strand, within DvMF_0317 at 369.352 kb on + strand, within DvMF_0317 at 369.563 kb on - strand, within DvMF_0317 at 369.972 kb on + strand at 370.002 kb on - strand, within DvMF_0318 at 370.109 kb on + strand, within DvMF_0318 at 370.109 kb on + strand, within DvMF_0318 at 370.117 kb on - strand, within DvMF_0318 at 370.117 kb on - strand, within DvMF_0318 at 370.283 kb on - strand, within DvMF_0318 at 370.288 kb on - strand, within DvMF_0318 at 370.369 kb on - strand, within DvMF_0318 at 370.376 kb on + strand, within DvMF_0318 at 370.509 kb on - strand, within DvMF_0318 at 370.513 kb on + strand, within DvMF_0318 at 370.559 kb on - strand, within DvMF_0318 at 370.637 kb on - strand at 370.796 kb on - strand at 370.899 kb on + strand at 370.956 kb on - strand at 371.266 kb on - strand, within DvMF_0319 at 371.295 kb on + strand, within DvMF_0319 at 371.344 kb on - strand, within DvMF_0319 at 371.434 kb on - strand at 371.443 kb on + strand at 371.451 kb on - strand at 371.542 kb on + strand at 371.542 kb on + strand at 371.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 369,065 + DvMF_0317 0.62 -0.4 369,182 + DvMF_0317 0.67 -0.3 369,258 - DvMF_0317 0.71 -1.3 369,258 - DvMF_0317 0.71 -0.5 369,278 - DvMF_0317 0.72 -0.7 369,282 - DvMF_0317 0.72 +1.1 369,352 + DvMF_0317 0.75 -0.9 369,563 - DvMF_0317 0.85 -0.1 369,972 + -1.1 370,002 - DvMF_0318 0.13 +1.6 370,109 + DvMF_0318 0.27 -0.4 370,109 + DvMF_0318 0.27 -0.7 370,117 - DvMF_0318 0.28 -1.9 370,117 - DvMF_0318 0.28 -0.5 370,283 - DvMF_0318 0.48 -0.6 370,288 - DvMF_0318 0.49 -1.5 370,369 - DvMF_0318 0.59 -0.5 370,376 + DvMF_0318 0.60 -0.5 370,509 - DvMF_0318 0.76 -0.6 370,513 + DvMF_0318 0.77 -1.0 370,559 - DvMF_0318 0.83 -0.5 370,637 - -0.2 370,796 - -1.3 370,899 + +0.9 370,956 - +0.2 371,266 - DvMF_0319 0.70 +0.6 371,295 + DvMF_0319 0.76 -2.8 371,344 - DvMF_0319 0.86 -3.1 371,434 - +0.3 371,443 + -0.6 371,451 - -0.6 371,542 + +1.7 371,542 + -1.3 371,550 - +0.8
Or see this region's nucleotide sequence