Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0221 and DvMF_0222 are separated by 155 nucleotides DvMF_0222 and DvMF_0223 overlap by 11 nucleotides
DvMF_0221: DvMF_0221 - OmpA/MotB domain protein (RefSeq), at 247,202 to 248,239
_0221
DvMF_0222: DvMF_0222 - FAD-dependent pyridine nucleotide-disulphide oxidoreductase (RefSeq), at 248,395 to 249,816
_0222
DvMF_0223: DvMF_0223 - glycine dehydrogenase subunit 2 (RefSeq), at 249,806 to 251,251
_0223
Position (kb) 248
249
250 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 247.439 kb on - strand, within DvMF_0221 at 248.075 kb on - strand, within DvMF_0221 at 248.107 kb on + strand, within DvMF_0221 at 248.404 kb on + strand at 248.557 kb on + strand, within DvMF_0222 at 248.844 kb on - strand, within DvMF_0222 at 248.908 kb on - strand, within DvMF_0222 at 248.924 kb on - strand, within DvMF_0222 at 248.931 kb on + strand, within DvMF_0222 at 248.931 kb on + strand, within DvMF_0222 at 248.933 kb on + strand, within DvMF_0222 at 248.941 kb on - strand, within DvMF_0222 at 248.946 kb on + strand, within DvMF_0222 at 249.031 kb on + strand, within DvMF_0222 at 249.115 kb on - strand, within DvMF_0222 at 249.374 kb on - strand, within DvMF_0222 at 249.469 kb on + strand, within DvMF_0222 at 249.558 kb on + strand, within DvMF_0222 at 249.582 kb on - strand, within DvMF_0222 at 249.673 kb on + strand, within DvMF_0222 at 249.696 kb on - strand at 249.696 kb on - strand at 249.850 kb on + strand at 249.902 kb on + strand at 249.927 kb on + strand at 249.927 kb on + strand at 250.049 kb on - strand, within DvMF_0223 at 250.214 kb on + strand, within DvMF_0223 at 250.465 kb on - strand, within DvMF_0223 at 250.597 kb on + strand, within DvMF_0223 at 250.605 kb on - strand, within DvMF_0223 at 250.697 kb on + strand, within DvMF_0223 at 250.713 kb on - strand, within DvMF_0223 at 250.766 kb on - strand, within DvMF_0223 at 250.785 kb on + strand, within DvMF_0223
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 247,439 - DvMF_0221 0.23 +1.1 248,075 - DvMF_0221 0.84 -2.7 248,107 + DvMF_0221 0.87 -0.9 248,404 + -1.0 248,557 + DvMF_0222 0.11 -0.7 248,844 - DvMF_0222 0.32 -0.6 248,908 - DvMF_0222 0.36 -0.8 248,924 - DvMF_0222 0.37 -1.3 248,931 + DvMF_0222 0.38 -0.1 248,931 + DvMF_0222 0.38 -0.3 248,933 + DvMF_0222 0.38 -0.8 248,941 - DvMF_0222 0.38 -1.1 248,946 + DvMF_0222 0.39 -1.2 249,031 + DvMF_0222 0.45 -2.1 249,115 - DvMF_0222 0.51 -0.3 249,374 - DvMF_0222 0.69 -0.1 249,469 + DvMF_0222 0.76 -3.7 249,558 + DvMF_0222 0.82 -1.8 249,582 - DvMF_0222 0.83 -0.8 249,673 + DvMF_0222 0.90 +2.3 249,696 - -0.2 249,696 - +0.1 249,850 + +0.7 249,902 + -0.3 249,927 + -0.8 249,927 + -1.9 250,049 - DvMF_0223 0.17 -0.5 250,214 + DvMF_0223 0.28 -0.7 250,465 - DvMF_0223 0.46 -0.4 250,597 + DvMF_0223 0.55 +0.7 250,605 - DvMF_0223 0.55 -0.1 250,697 + DvMF_0223 0.62 -0.7 250,713 - DvMF_0223 0.63 -0.7 250,766 - DvMF_0223 0.66 -0.9 250,785 + DvMF_0223 0.68 -4.4
Or see this region's nucleotide sequence