Experiment: MoYLS4 with Doxycycline hyclate 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0131 and DvMF_0132 are separated by 2 nucleotides DvMF_0132 and DvMF_0133 are separated by 127 nucleotides DvMF_0133 and DvMF_0134 are separated by 206 nucleotides DvMF_0134 and DvMF_0135 are separated by 113 nucleotides
DvMF_0131: DvMF_0131 - hypothetical protein (RefSeq), at 147,498 to 147,839
_0131
DvMF_0132: DvMF_0132 - metal-dependent phosphohydrolase HD region (RefSeq), at 147,842 to 148,711
_0132
DvMF_0133: DvMF_0133 - Methyltransferase type 11 (RefSeq), at 148,839 to 149,576
_0133
DvMF_0134: DvMF_0134 - hypothetical protein (RefSeq), at 149,783 to 150,322
_0134
DvMF_0135: DvMF_0135 - Haloacid dehalogenase domain protein hydrolase (RefSeq), at 150,436 to 151,254
_0135
Position (kb) 148
149
150 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 147.882 kb on - strand at 148.010 kb on + strand, within DvMF_0132 at 148.019 kb on + strand, within DvMF_0132 at 148.048 kb on - strand, within DvMF_0132 at 148.073 kb on - strand, within DvMF_0132 at 148.275 kb on + strand, within DvMF_0132 at 148.364 kb on + strand, within DvMF_0132 at 148.372 kb on - strand, within DvMF_0132 at 148.818 kb on + strand at 148.894 kb on - strand at 149.743 kb on + strand at 149.845 kb on - strand, within DvMF_0134 at 149.935 kb on + strand, within DvMF_0134 at 149.942 kb on + strand, within DvMF_0134 at 149.943 kb on - strand, within DvMF_0134 at 150.050 kb on + strand, within DvMF_0134 at 150.215 kb on - strand, within DvMF_0134 at 150.362 kb on - strand at 150.410 kb on - strand at 150.426 kb on + strand at 150.545 kb on - strand, within DvMF_0135
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Doxycycline hyclate 0.0156 mg/ml remove 147,882 - -3.6 148,010 + DvMF_0132 0.19 -0.2 148,019 + DvMF_0132 0.20 -3.4 148,048 - DvMF_0132 0.24 -0.9 148,073 - DvMF_0132 0.27 -2.9 148,275 + DvMF_0132 0.50 -0.6 148,364 + DvMF_0132 0.60 -0.9 148,372 - DvMF_0132 0.61 +1.9 148,818 + +0.0 148,894 - +1.6 149,743 + +0.0 149,845 - DvMF_0134 0.11 -1.0 149,935 + DvMF_0134 0.28 -1.0 149,942 + DvMF_0134 0.29 -0.5 149,943 - DvMF_0134 0.30 -0.5 150,050 + DvMF_0134 0.49 +0.4 150,215 - DvMF_0134 0.80 +0.3 150,362 - -0.4 150,410 - -2.7 150,426 + -3.8 150,545 - DvMF_0135 0.13 -1.5
Or see this region's nucleotide sequence