Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2457 and Shew_2458 are separated by 239 nucleotides Shew_2458 and Shew_2459 are separated by 243 nucleotides Shew_2459 and Shew_2460 are separated by 106 nucleotides
Shew_2457: Shew_2457 - nuclease SbcCD, D subunit (RefSeq), at 2,889,519 to 2,890,661
_2457
Shew_2458: Shew_2458 - glutathione S-transferase domain-containing protein (RefSeq), at 2,890,901 to 2,891,761
_2458
Shew_2459: Shew_2459 - hypothetical protein (RefSeq), at 2,892,005 to 2,892,352
_2459
Shew_2460: Shew_2460 - hypothetical protein (RefSeq), at 2,892,459 to 2,893,502
_2460
Position (kb)
2890
2891
2892 Strain fitness (log2 ratio)
-1
0
1 at 2889.977 kb on + strand, within Shew_2457 at 2890.029 kb on + strand, within Shew_2457 at 2890.222 kb on - strand, within Shew_2457 at 2890.404 kb on + strand, within Shew_2457 at 2890.604 kb on + strand at 2890.612 kb on - strand at 2890.754 kb on - strand at 2891.040 kb on + strand, within Shew_2458 at 2891.696 kb on + strand at 2891.728 kb on - strand at 2891.751 kb on - strand at 2891.837 kb on + strand at 2892.242 kb on + strand, within Shew_2459 at 2892.465 kb on - strand at 2892.581 kb on + strand, within Shew_2460 at 2892.582 kb on - strand, within Shew_2460 at 2892.589 kb on - strand, within Shew_2460
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 2,889,977 + Shew_2457 0.40 +0.1 2,890,029 + Shew_2457 0.45 +0.3 2,890,222 - Shew_2457 0.62 +0.5 2,890,404 + Shew_2457 0.77 +0.1 2,890,604 + +0.4 2,890,612 - +0.7 2,890,754 - +1.1 2,891,040 + Shew_2458 0.16 -1.2 2,891,696 + +0.1 2,891,728 - -0.0 2,891,751 - +0.1 2,891,837 + +0.6 2,892,242 + Shew_2459 0.68 -0.0 2,892,465 - +0.0 2,892,581 + Shew_2460 0.12 -0.4 2,892,582 - Shew_2460 0.12 +1.0 2,892,589 - Shew_2460 0.12 -0.6
Or see this region's nucleotide sequence